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正交试验法优选黄芪中总黄酮提取工艺(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2012年01期
页码:
27-29
栏目:
实验研究
出版日期:
2012-02-29

文章信息/Info

Title:
Studing on Extraction Condition of Total Flavonoids from Radix Astragali by Orthogonal Test Method
作者:
赵琦1张军武2
1.咸阳市卫生学校,陕西咸阳712000;2.陕西中医学院,陕西咸阳712046
Author(s):
ZHAO-Qi1 ZHANG Jun-Wu2
1.Health School of Xianyang, Xianyang shanxi 712000,China; 2.Shanxi University of Chinese Medicine, Xianyang shanxi 712046,China
关键词:
正交试验法黄芪总黄酮提取工艺
Keywords:
orthogonal test methodradix Astragalitotal flavonoidsextraction process
分类号:
R284·2
DOI:
-
文献标识码:
A
摘要:
目的:优选黄芪总黄酮的提取工艺条件。方法:采用正交试验法对黄芪总黄酮提取工艺条件进行优选,以总提物和总黄酮含量为指标,考察影响提取指标的4个因素,即溶媒用量(A),乙醇浓度(B),提取时间(C),提取次数(D);每个因素取3个水平。结果:用乙醇提取黄芪总黄酮的最佳工艺为A2B2C1D2,即用10倍量70%乙醇回流提取2次,每次60min。结论:此法稳定可行,可为黄芪总黄酮提取工艺的确定提供依据。
Abstract:
Objective: Select the best condition for extraction process of total flavonoids from Radix Astragali. Method: Regard the total extract yield and the yield of total flavonoids as indicators, total flavonoids extraction conditions were optimized by using orthogonal test method. Four factors were examined by three levels: the amount of solvent(A), ethanol concentration(B), extraction time(C),extraction frequency(D).Results: The best extraction process of total flavonoids from Radix Astragali by ethanol is A2B2C1D2,that is,it was extracted with 10 times the amount of 70% ethanol two times, 60min every time. Conclusion: The method can provide a stable basis for extraction process of total flavonoids from Radix Astragali.

参考文献/References

[1]国家药典委员会.中国药典:一部[M].北京:中国医药科技出版社,2010.
[2]齐宗韶.黄芪化学成分的研究概况[J].中草药,1987,18(5):41.
[3]汪德清,丁保国,Tomas G Neil,等.黄芪总黄酮对动脉粥样硬化早期形成的影响[J].中国药理学通报,2003,19(6):637-640.
[4]纪松岗,李翔,娄子洋,等.正交设计法确定黄芪中黄酮类成分的超声提取条件[J].第二军医大学学报,2005,26(10):1168-1169.

备注/Memo

备注/Memo:
基金项目:陕西省中药制药学重点学科资助项目 收稿日期:2011—08—29修回日期:2011—09—30 作者简介:赵琦(1978~),女,河南偃师人,讲师,研究方向为中药抗衰老机理及新药研究。△通讯作者:张军武,E-mail:zjw19982005@163com.
更新日期/Last Update: 2012-02-29