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|本期目录/Table of Contents|

黄芪对兔肺缺血再灌注损伤保护作用的病理观察(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2012年03期
页码:
37-39
栏目:
实验研究
出版日期:
2012-06-30

文章信息/Info

Title:
Protective Effects of Astragali(Huangqi) on the Pathomorphologic Changes of Ischemia/Reperfusion Lung Injury
作者:
姚华宁邹积俊张家衡
武汉市中西医结合医院,湖北武汉430022
Author(s):
YAO Hua-ning ZOU Ji-jun ZHANG Jia-heng
Wuhan Integrated Traditional and Western Medicine Hospital, Wuhan Hubei ,430022
关键词:
再灌注损伤病理学黄芪:免疫组化
Keywords:
lungischemia/reperfusion injuryhistopathologyastragaliimmunohistichemistry
分类号:
R285.5
DOI:
-
文献标识码:
A
摘要:
目的:探讨黄芪减轻肺缺血再灌注损伤的机制。方法:将24只健康大白兔随机分两组,肺缺血再灌注模型组(12只)、黄芪干预组(12只),气管切开后利用人工呼吸机行单肺通气,左肺门阻断2h后,恢复双肺通气4h后左肺下叶组织取材,应用光镜、电镜和免疫组织化学染色等病理检测方法观察肺缺血再灌注后肺组织病理形态学和免疫表型改变。计算受损肺泡百分率。结果:模型组肺缺血再灌注后左肺出血加重,肺间质及广泛肺泡腔内有水肿液,肺泡上皮细胞肿胀,线粒体广泛空泡化和嵴溶解。同例对照右肺大致正常。干预组因术前使用黄芪汤,左肺组织结构完整,少数肺泡上皮细胞线粒体轻度肿胀和空泡化。黄芪干预组受损肺泡百分率(P<005)和半胱氨酸天冬氨酸特异性蛋白酶-3表达量明显低于缺血再灌注模型组(P<001)。结论:中药黄芪对兔肺缺血再灌注损伤有一定的保护作用。
Abstract:
Objective To investigate the protective effects of Astragali on lung ischemia/reperfusion(I/R) injury in rabbits. Methods 24 rabbits were randomly divided into 2 groups-I/R group and intervention group. Rabbits were killed after the left lung was blocked for 2h and followed by 4h of ventilation. Astragali (2ml/kg) were fed before operation. Lung pathomorphologic and immunotype changes were observed and the percentage of damage alveoli was measure. Result In I/R group after reperfusion, the left lung became diffusely hemorrhagic and produced interstitial or alveolar edema. More serious mitochondria vacuolization and ridge dissolution were appeared in some alveolar epithelial cells. The right lung as control as normal. In intervention group, the left lung showed mild mitochondria vacuolization in a few alveolar epithelial cells .The percontage of elamage alveoli of in the left lung of the intervention group was significanthy lower than that of the I/R group (P<005). The alveolar epithelial of the I/R group cell overexpressed Caspase-3(P<001).Conclusion Astragali has a protective effect for the experimental rabbit lung ischemia reperfusion injury.

参考文献/References

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备注/Memo

备注/Memo:
基金项目:湖北省卫生厅科研项目(NO:2007(56)号) 收稿日期:2011—09—28修回日期:2012—05—29
更新日期/Last Update: 2012-07-10