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白及须根与块茎的多糖成分比较研究(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2015年02期
页码:
29-32,52
栏目:
方药研究
出版日期:
2015-04-25

文章信息/Info

Title:
Comparative Study of Polysaccharide in Fibrous Root and Tuber of Bletilla striata
作者:
俞杭苏史珍珍吕迪潘平钱朝东陈建真丁志山△
浙江中医药大学,浙江 杭州 310053
Author(s):
YU Hang-su SHI Zhen-zhen LYU Di PAN Ping QIAN Chao-dong CHEN Jian-zhen DING Zhi-shan△
Zhejiang Chinese Medical University, Hangzhou 310053, China
关键词:
白及 须根 块茎 多糖
Keywords:
Bletilla striata fibrous roots tubers polysaccharide
分类号:
R284
DOI:
-
文献标识码:
A
摘要:
目的比较白及须根与块茎中多糖的含量及组成。方法采用水提醇沉法提取须根、块茎中的多糖成分;苯酚-浓硫酸法测定须根与块茎的总糖含量;DNS测定须根与块茎中还原糖含量;运用GC-MS分析白及须根与块茎中多糖的单糖组分。结果白及须根总糖含量低于块茎中含量,在总糖中还原性糖含量高于块茎;白及须根与块茎中的多糖中都含有葡萄糖和甘露糖,但须根中还含有半乳糖。结论白及须根和块茎中总糖、还原糖、单糖成分有差异,白及须根中总糖含量没有块茎中高,但具有一定的潜在药用价值,值得进一步开发研究。
Abstract:
Objective Compare the content and composition of polysaccharide in fibrous root and tuber of Bletilla striata(Thunb. ) Reichb. f. . Methods Extract the polysaccharide of the fibrous root and tuber of Bletilla striata with water extraction and ethanol precipitation; Phenol-sulfuric acid method and 3, 5-dinitrosalicylic acid(DNS) method were carried out to evaluate the polysaccharide of the pseudobulb part of Bletilla striata; using GC-MS analysis of fibrous root and tuber of monosaccharide composition of polysaccharides. Results The fibrous root polysaccharide content is lower than the polysaccharide content in the tubers; The reducing sugar content is higher than the tuber; The fibrous root and tuber of polysaccharide containing glucose and mannose, but also contain galactose in fibrous root. Conclusion The fibrous root and tuber of Bletilla striata in the content of total sugar, reducing sugar, monosaccharide composition are plants. This is regrettable because phytoalexins or different, polysaccharide content of tubers is more than fibrous, but has certain potential medicinal value, is worth further development.

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备注/Memo

备注/Memo:
基金项目: 浙江省新苗人才计划(2012R410038);2013年地方高校国家级大学生创新创业训练计划项目(201310344004) 收稿日期: 2014 - 12 - 11 作者简介: 俞杭苏(1990-),女,浙江新昌人,在读硕士研究生,研究方向:中药活性成分研究及新药开发。△通信作者:丁志山,E-mail:zjtcmdzs@sohu.com
更新日期/Last Update: 2015-04-30