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益骨汤对骨质疏松大鼠骨密度及BMP-2信号通路的影响(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2016年06期
页码:
5-9
栏目:
实验研究
出版日期:
2016-12-10

文章信息/Info

Title:
Effects of YiGu Recipe on the Expression of BMP-2 Signaling Pathways in Rats with Osteoporosis
作者:
李威姚新苗△李晓亚俞斌董正超
浙江中医药大学第三临床医学院,浙江 杭州 310053
Author(s):
LI Wei YAO Xinmiao LI Xiaoya YU Bin DONG Zhengchao
College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China
关键词:
益骨汤 骨质疏松 BMP-2信号通路 Noggin
Keywords:
Yigu Recipe Osteoporosis BMP-2 signaling pathway Noggin
分类号:
R285.5
DOI:
10.19288/j.cnki.issn.1000-2723.2016.06.002
文献标识码:
A
摘要:
目的 研究益骨汤对骨质疏松大鼠BMP-2信号通路上BMP-2(Bone morphogenetic protein-2)、Smads、Runx2(Runt-related transcription factor 2)、OSX(Osterix)、Noggin基因转录及蛋白表达的影响,探究益骨汤防治骨质疏松症作用机制。方法 采用去除大鼠卵巢方法,建立骨质疏松症模型。分空白组、模型组、补佳乐组、益骨汤组、Noggin反义寡核苷酸组。以实时定量PCR检测BMP-2通路基因转录,WesternBlot法检测BMP-2通路蛋白表达。结果 ①模型组骨密度值明显低于空白组(P<0.01),益骨汤组、补佳乐组、Noggin反义寡核苷酸组骨密度值明显高于模型组(P<0.01)。②益骨汤组、补佳乐组、Noggin反义寡核苷酸组大鼠BMP-2、Smads、Runx2、OSXmRNA表达明显高于模型组(P<0.05);而益骨汤组Noggin表达明显低于模型组(P<0.05)。③益骨汤组、补佳乐组、Noggin反义寡核苷酸组大鼠BMP-2、Smads、Runx2、OSX蛋白条带明显深于模型组,而益骨汤组Noggin蛋白条带明显浅于模型组。结论 益骨汤防治骨质疏松症机制与上调BMP-2、Smads、Runx2、OSX表达,同时抑制Noggin过度表达以及增加骨密度有关。推测BMPs抑制剂Noggin可能是新的抗骨质疏松靶点。
Abstract:
Objective To study the effects of YiGu Recipe on the mRNA transcription and protein expression of BMP-2(Bone morphogenetic protein-2), Smads, Runx2(Runt-related transcription factor 2), OSX(Osterix), Noggin on BMP-2 signaling pathways in rats with osteoporosis, analyze the mechanism of Yigu Recipe in the prevention and treatment of osteoporosis. Methods The osteoporosis rats model was built by the ovariectomy. All rats were divided into normal control, blank model, progynova, YiGu Recipe, anti-Noggin group. RT-PCR and WesternBlotting methods was applied to measure the mRNA and protein expression of BMP-2, Smads, Runx2, OSX, Noggin mRNA in bone homogenate. Results First, the BMD(Bone mineral density) of the model group significantly decreased compared with the blank group(P<0.01), the BMD significantly increased compared with the model group(P<0.01). Second, the expression of BMP-2、Smads、Runx2、OSX mRNA significantly increased compared with the model group(P<0.05), the expression of Noggin mRNA significantly decreased compared with the model group(P<0.05). At last, the expression of BMP-2, Smads, Runx2, OSX protein significantly increased compared with the model group, the expression of Noggin protein significantly decreased compared with the model group. Conclusion The mechanism of Yigu Recipe in the prevention and treatment of osteoporosis was increase the BMD, inhibiting the expression of Noggin and up-regulating the expression of the BMP-2, Smads, Runx2, OSX. Noggin as an inhibitor of MMPs may be a novel anti-osteoporosis target.

参考文献/References

[1] Roush K. Prevention and treatment of osteoporosis in postmenopausal women:a review[J]. Am J Nurs,2011,111(8):26-35.
[2] Adachi JD,Adami S,Gehlbach S,et al. GLOW Investigators. Impact of prevalent fractures on quality of life:baseline results from the global longitudinal study of osteoporosis in women[J]. Mayo Clin Proc,2010,85(9):806-813.
[3] 郎名丽,尉双玲. 骨质疏松的药物治疗进展[J]. 中国卫生产业,2014,59(9):93-94.
[4] 张鑫,肖鲁伟,童培建. 补肾活血汤防治绝经后骨质疏松症的实验研究[J]. 中医正骨,2010,22(2):3-6.
[5] 金珉庭,郑洪新,朱辉. 补肾中医药对骨质疏松症大鼠下丘脑BMP-4、Smad6 mRNA及蛋白表达的影响[J]. 中国骨质疏松杂志,2008,14(8):556-560.
[6] 蔡玉霞,张剑宇. 补骨脂水煎剂对去卵巢骨质疏松大鼠骨代谢的影响[J]. 中国组织工程研究与临床康复,2009,13(2):268-271.
[7] 丁小刚,覃勇,鄂建设,等. 骨碎补总黄酮对老年性骨质疏松症患者血清骨钙素水平及骨密度影响[J]. 中国骨质疏松杂志,2013,19(5):519-521.
[8] 李青南,吴铁,谢华,等. 淫羊藿提取液对去睾丸大鼠骨代谢的影响[J]. 中草药,1993,24(12):637.
[9] 李青南,廖进民,吴铁,等. 淫羊藿提取液防治激素所致大鼠骨质疏松的实验研究[J]. 中国药学杂志,1996,31(8):467-470.
[10] 肖柳斌,刘国雄,王晖. 丹参水提液对抗大鼠泼尼松性骨质疏松的作用研究[J]. 中国临床药理学与治疗学,2008,13(1):94-98.
[11] 杨林,姚新苗,黄竞,等. 益骨汤对去卵巢大鼠血清激素及成骨细胞增殖的影响[J]. 中医正骨杂志,2006,18(12):8-9.
[12] 姚新苗,朱胤晟,应建伟. 益骨汤口服液抑制去势大鼠骨质疏松疼痛的实验研究[J]. 浙江中医药大学学报,2013,37(8):1005-1009.
[13] 朱胤晟,姚新苗,吕一. 益骨汤口服液对去势大鼠骨质疏松症血瘀病机微观分子的影响[J]. 江苏中医药,2013,45(5),71-72
[14] 姚新苗,朱胤晟,平佃辉,等. 益骨汤对去势大鼠骨质疏松症外周血清炎性因子的影响[J]. 中医正骨,2012,24(11):7-8.
[15] 姚新苗,陈于东,方芳. 益骨汤含药血清对成骨细胞增殖和ALP影响的实验研究[J]. 浙江中医药大学学报,2007,31(2):158-159.
[16] Cao X. ,Chen D. The BMP signaling and in vivo bone formation[J]. Gene,2005,357(1):1-8.
[17] K. Miyazono,Y. Kamiya,M. Morikawa. Bone morphogenetic protein receptors and signal transduction[J]. Journal of biochemistry,2010,147(1):35-51.
[18] Nohe A,Keating E,Knaus P,et a1. Signal transduction of bone morphogenetie protein receptors[J]. Cell Signal,2004,16(3):291-299.
[19] Jeon EJ,Lee KY,Choi NS,et a1. Bone morphogenetie protein-2 stimulates Runx2 aoetylation[J]. J Biol Chem,2006,281(24):16502-16511.
[20] Canalis E. Update in new anabolic the rapiles for osteoporosis[J]. J Clin Endocrinol metab,2010,95(4):1496-1504.

备注/Memo

备注/Memo:
* 基金项目: 浙江省自然科学基金重点项目(LZ15H270001) 收稿日期: 2016 - 09 - 30 作者简介: 李威(1990-),男,河南安阳人,在读硕士研究生,研究方向:中医骨伤科基础与临床。△通信作者:姚新苗,E-mail:fengxieqing10yang@163.com
更新日期/Last Update: 2016-11-20