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通督调神电针法对脑梗死模型大鼠GAP-43表达的影响(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2016年06期
页码:
21-25
栏目:
实验研究
出版日期:
2016-12-10

文章信息/Info

Title:
Effect of Tongdu Adjustable God Electroacupuncture on Expressions of GAP-43in Cerebral Infarction Model Rats
作者:
丁义侠陈亮亮储浩然
安徽中医药大学第二附属医院,安徽 合肥 230061
Author(s):
DING Yixia CHEN Liangliang CHU Haoran
The Second Affiliated Hospital of Anhui TCM University, Hefei 230061, China
关键词:
通督调神 电针 脑梗死 GAP-43
Keywords:
tongdu adjustable god electroacupuncture cerebral infarction GAP-43
分类号:
R246.6
DOI:
10.19288/j.cnki.issn.1000-2723.2016.06.006
文献标识码:
A
摘要:
目的 观察通督调神电针法对脑梗死模型大鼠GAP-43表达的影响,探索其对神经功能再塑的影响机制,为临床运用通督调神电针法治疗脑梗死提供实验依据。方法 SPF级雄性大鼠64只,体质量250~280g,采用随机数字法随机分为4个大组,每组16只大鼠。每个大组再随机分为2个亚组,选取7d和14d两个时间点,每组8只。用电凝法制备脑梗死模型。电针组针刺主穴为百会、水沟、大椎、神庭,每天针刺1次,留针30min。模型组、假手术组、正常对照组予以同一时间捆绑固定不予针刺处理。最后免疫组化法观察每组大鼠7d、14d两个时间点梗死灶周围GAP-43阳性细胞的表达,采用JD801形态学图像分析系统进行分析,并对结果进行统计学分析。结果 正常对照组和假手术组中无GAP-43阳性细胞,模型组在7d和14d两个时间点与假手术组比较有极显著意义(P<0.01);模型组与电针治疗组比较,在7d和14d两个时间点电针组的GAP-43的阳性细胞均较模型组多,且两组差别有极显著意义(P<0.01)。结论 通督调神电针法可促进各时间点脑梗死大鼠脑组织内GAP-43的表达。
Abstract:
Objective To observe the tongdu adjustable god electroacupuncture on the neural plasticity in rats with cerebral infarction and explore its mechanism, and to provide experimental evidence for the treatment of ischemic cerebrovascular disease. Methods Choose SPF male 64 rates, weight 250~280g rats. The experimental rats were divided into four groups, 16 rats in every group. Each group were randomly divided into 7d, 14d two sub group, 8 rats in each sub group. Cerebral infarction model was prepared by electric coagulation method. electroacupuncture group acupuncture GV20, GV26, GV14 and GV24, 30 minutes for each time. Model group, sham operation group and normal control group were given the same time bound and fixed not acupuncture treatment. The immune group of method to detect GAP-43 expression, by JD801 morphological image analysis system were analyzed and the results were statistically analyzed. Results No GAP-43 positive cells in normal control group and sham operation group, Model group at 7 and 14 days two time points compared with the sham operation group had significant meaning(P<0.01); Compared with electric acupuncture treatment group, model group at 7 d and 14 d two time points GAP-43 positive cells in electric acupuncture group were compared with model group, and the difference between the two groups have significant meaning(P<0.01). Conclusion The expression of GAP-43 in brain tissue of rats with cerebral infarction was promoted by the regulation of the tongdu adjustable god electroacupuncture.

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备注/Memo

备注/Memo:
* 基金项目: 安徽中医药大学青年科研研究基金(2014qn032) 收稿日期: 2016 - 11- 10 作者简介: 丁义侠(1985-),女,安徽旌德人,医学硕士,主治医师,研究方向:神经系统疾病的针灸治疗。通信作者:陈亮亮,E-mail:Chenliangliang0082@126.com
更新日期/Last Update: 2016-11-20