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健脾清肠方含药血清对小鼠小肠Cajal间质细胞增殖的影响(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2017年01期
页码:
1-4
栏目:
实验研究
出版日期:
2017-02-20

文章信息/Info

Title:
Effect of Jianpi Qingchang Decoction Containing Serum on Proliferationof Mice Intestinal Interstitial Cells of Cajal in Vitro
作者:
戴彦成1张亚利1郑烈1王立娟2唐志鹏1
1.上海中医药大学附属龙华医院消化内科/上海中医药大学脾胃病研究所 上海 200032;2.上海中医药大学教学实验中心,上海 201203
Author(s):
DAI Yancheng1ZHANG Yali1ZHENG Lie1WANG Lijuan2TANG Zhipeng1
1. Department of Gastroenterology, Institute of Digestive Disease, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China;2. Teaching and Experiment Center Shanghai University of Traditional Chinese Medicine, Shanghai 201203
关键词:
健脾清肠方 Cajal间质细胞 增殖 溃疡性结肠炎
Keywords:
Jianpi Qingchang decoction interstitial cells of Cajal proliferation ulcerative colitis
分类号:
R285.5
DOI:
10.19288/j.cnki.issn.1000-2723.2017.01.001
文献标识码:
A
摘要:
目的 研究健脾清肠方含药血清(Jianpi Qingchang Decoction containing serum,JQD-CS)对小鼠小肠Cajal间质细胞(interstitial cells of Cajal,ICC)增殖的影响。方法 体外培养小鼠小肠ICC并鉴定,制备JQD-CS,MTT检测不同浓度JQD-CS对ICC的增殖作用。结果 适度浓度(≤20%)的JQD-CS,ICC的OD值随着含药血清浓度的增加而升高。结论 适度浓度的JQD-CS可对ICC增殖分化起促进作用。
Abstract:
Objective To evaluate the effect of Jianpi Qingchang Decoction containing serum (JQD-CS) on proliferation of mice intestinal interstitial cells of Cajal(ICC) in vitro. Methods Cultivation and identification of mice intestinal ICC in vitro;preparation of JQD-CS. MTT method detected the proliferation of ICC intervened by different concentrations of JQD-CS. Results Moderate concentration(less than 20%) of the JQD-CS, the OD value of ICC increase with the increasing concentration of serum. Conclusion Moderate concentration of JQD-CS can promote the proliferation and differentiation of ICC.

参考文献/References

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备注/Memo

备注/Memo:
* 基金项目: 国家自然科学基金(81403355,81573892);上海市中医药新三年行动计划项目(ZY3-RCPY-2-2001)
收稿日期: 2016 - 12- 22
作者简介: 戴彦成(1982-),男,江苏扬中人,博士,主治医师,研究方向:中医药治疗炎症性肠病。
△通信作者:唐志鹏,E-mail:zhipengtang@sohu.com
更新日期/Last Update: 2017-02-10