我们的网站为什么显示成这样?

可能因为您的浏览器不支持样式,您可以更新您的浏览器到最新版本,以获取对此功能的支持,访问下面的网站,获取关于浏览器的信息:

|本期目录/Table of Contents|

化瘀通络灸法对皮质下血管性痴呆大鼠额叶皮质下白质TNF-α、IL-1β表达的影响(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2017年01期
页码:
5-9
栏目:
实验研究
出版日期:
2017-02-20

文章信息/Info

Title:
Effect of Huayu Tongluo Moxibustion on the Expressions of Frontal SubcorticalTNF-α、IL-1β in Subcortical Vascular Dementia (SVD) Rats
作者:
张风云1张庆萍2吴生兵3王明洁1
1. 安徽中医药大学,安徽 合肥 230038;2. 安徽中医药大学针灸骨伤临床学院,安徽 合肥 230038;3. 新安医学教育部重点实验室,安徽 合肥 230038
Author(s):
ZHANG Fengyun1ZHANG Qingping2WU Shengbing3WANG Mingjie1
Anhui University of Traditional Chinese Medicine, Hefei 230038, China
关键词:
血管性痴呆 化瘀通络灸法 肿瘤坏死因子-α 白介素-1β
Keywords:
vascular dementia Huayu Tongluo Moxibustion tumor necrosis factor-α Interleukin-1β
分类号:
R245.81
DOI:
10.19288/j.cnki.issn.1000-2723.2017.01.002
文献标识码:
A
摘要:
目的 观察化瘀通络灸法对皮质下血管性痴呆(subcortical vascular dementia,SVD)模型大鼠治疗作用及其对额叶皮质下白质炎性因子的影响。方法 采用Pulsinelli4血管阻断法稍加改进复制大鼠血管性痴呆模型,电脑随机数字表法随机分为3组:艾灸组、药物组、模型组,并设假手术组、正常组对照。艾灸组取百会、大椎、神庭穴艾灸治疗,药物组多奈哌齐灌胃治疗。每日1次,连续治疗4周。采用跳台仪检测治疗后学习记忆成绩;采用免疫组化检测额叶皮质下白质肿瘤坏死因子-α(tumor necrosis factor-alpha,TNF-α)、白介素-1β(Interleukin-1beta,IL-1β)阳性细胞表达。结果 ①大鼠跳台实验的潜伏期、错误次数,艾灸组及药物组与模型组比较差异具有统计学意义(P<0.01,P<0.05),且艾灸组明显优于药物组(P<0.01,P<0.05)。②艾灸组及药物组与模型组比,大鼠额叶皮质下白质TNF-α、IL-1β表达差异均有统计学意义。艾灸组与药物组比较TNF-α、IL-1β表达差异有统计学意义(P<0.05)。结论 化瘀通络灸法治疗SVD可能是通过抑制炎性细胞因子TNF-α、IL-1β的产生,从而改善大鼠认知功能而实现的。
Abstract:
Objective To observe the effect of Huayu Tongluo Moxibustion on the treatment of subcortical vascular dementia(SVD) rats, and its effect on frontal subcortical inflammatory factors. Methods SVD rat models were established according to pulsinelli’s 4-vessel occlusion technique. The model rats were divided into 3 groups by the random digit table, i.e. , the moxibustion group, the medicine group, and the model group. The sham-operaton group and normal group was also set up. In the moxibustion group rats was acupunctured at Baihui(GV20), Dazhui(GV24), and Shenting(GV14). Donepezil was given to rats in the medicine group by gastrogavage. The above two groups were treated once a day for four weeks. The learning and memory results were observed by step-down avoidance test after treatment. The expressions of frontal subcortical TNF-α, IL-1β positive cells were detected using immunohistochemical assay. Results ① Statistical difference existed in the latent period, the error times of jumping experiment in the moxibustion group and the medicine group when compared with the model group(P<0.01, P<0.05). The moxibustion group was better than the medicine group(P<0.01, P<0.05). ② Expression differences of rats frontal subcortical TNF-α, IL-1β when compared the moxibustion group, medicine group with model group have statistical meaning. Also Expression differences of TNF-α, IL-1β when compared the moxibustion group with the medicine group have statistical meaning(P<0.05). Conclusion Using Huayu Tongluo Moxibustion to treat SVD may be acheived by restraining the appearance of inflammatory cytokines TNF-α, IL-1β to improve rats’ cognitive function.

参考文献/References

[1] Thal DR,Ghebremedhin E,Orantes M,et al. Vascular pathology in Alzheimer’s disease:correlation of cerebral amyloid angiopathy and arteriosclerosis/lipohyalinosis with cognitive decline[J]. J Neuropathol Exp Neurol,2003,62(12):1287-1301.
[2] Chui H. Dementia due to subcortical ischemic vascular disease[J]. Clinical Cornerstone,2001,3(4):40-51.
[3] S Engelborghs,N Le Bastard,B Feyen,et al. Overdiagnosis of vascular dementia using structural brain imaging in the context of standard clinical diagnostic criteria[J]. Alzheimer’s Dementia:the journal of the Alzheimer’s association,2011,7(4):S338-S339.
[4] 陈浩,王频,杨骏,等. 艾灸对血管性痴呆患者症状及脑脊液中神经肽类物质水平的影响[J]. 中国针灸,2011,31(1):19-22.
[5] 王频,杨骏,杨帆,等. 化瘀通络灸法治疗血管性痴呆临床观察[J]. 中国中医急症,2009,18(2):172-174.
[6] 王频,杨骏,柳刚,等. 艾灸头部穴位为主对血管性痴呆患者脑脊液中生长抑素和精氨酸血管加压素水平影响的随机对照试验[J]. 中西医结合学报,2010,8(7):636-640.
[7] 王频,杨骏,杨帆,等. 艾灸头部组穴治疗血管性痴呆的临床研究[J]. 中华中医药杂志,2009,24(10):1348-1350.
[8] 王明洁,张庆萍,吴生兵,等. 化瘀通络灸法对皮质下血管性痴呆额叶超微结构的影响[J]. 针灸临床杂志,2015,31(11):59-61.
[9] Pulsinelli WA,Brierley JB. A new model of bilateral hemispheric ischemia in the unanesthetized rat[J]. Stroke,1979, 10(3):267-272.
[10] Lok J,Gupta P,Guo S,et al. Cell-cell signaling in the neurovascular unit[J]. Neurochem Res,2007,32(12):2032-2045.
[11] Benarroch EE. Oligodendrocytes:Susceptibility to injury and involvement in neurologic disease[J]. Neurology,2009,72(20):1779-1785.
[12] Sairanen TR,Lindsberg PJ,Brenner M,et al. Differential cellular expression of tumor necrosis factor-alpha and Type I tumor necrosis factor receptor after transient global forebrain ischemia[J]. J Neurol Sci,2001,186(1-2):87-99.
[13] 季杰,王麟鹏,刘慧林. TNF-α、IL-1β与缺血性脑血管病的关系[J]. 首都医药,2005,12(10):23-25.
[14] 周艳丽,滕军放,赵莘瑜,等. NBP对脑I/R损伤大鼠脑组织中TNF-α、HMGB-1 mRNA表达的影响及意义[J]. 山东医药,2009,49(30):37-38.
[15] Angelopoulos P,Agouridaki H,Vaiopoulos H,et al. Cytokines in Alzheimer’s disease and vascular dementia[J]. Internetional Journal of Neuroscience,2008,118(12):1659-1672.
[16] Liu T,Clark RK,McDonnell PC,et al. Tumor necrosis factor-alpha expression in ischemic neurons[J]. Stroke,1994,25(7):1481-1488.
[17] Huang Y,Erdmann N,Peng H,et al. The role of TNF related apoptosis-inducing ligand in neurodegenerative diseases[J]. Cell Mol Immunol,2005,2(2):113-122.

备注/Memo

备注/Memo:
* 基金项目: 国家自然科学基金(81373744)
收稿日期: 2016 - 11- 28
作者简介: 张风云(1991-),男,安徽芜湖人,在读硕士研究生,研究方向:针灸临床应用及机理研究。
△通信作者:张庆萍,E-mail:zhangqp66@163.com
更新日期/Last Update: 2017-02-10