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肺癌微环境脾气虚证肿瘤组织相关巨噬细胞microRNA的表达及加味黄芪建中汤干预机制研究(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2017年02期
页码:
11-17
栏目:
实验研究
出版日期:
2017-04-30

文章信息/Info

Title:
The Expression of Tumor Associated Macrophages MicroRNA in Lung Cancer Microenvironment Spleen Deficiency Syndrome and the Influence of Modified Huangqi Jianzhong Decoction
作者:
魏自太12包素珍1李恒楠1李小东1
1. 浙江中医药大学,浙江 杭州 310053;2. 衢州职业技术学院,浙江 衢州 324000
Author(s):
WEI Zitai12 BAO Suzhen1 LI Hengnan1 LI Xiaodong1
1. Zhejiang Chinese Medical University, Hangzhou 310053, China; 2. Quzhou College of Technology, Quzhou 324000, China
关键词:
脾气虚 加味黄芪建中汤 微环境 肿瘤相关巨噬细胞microRNA
Keywords:
Spleen Qi deficiency modified Huangqi Jianzhong decoction microenvironment tumor-associated macrophages microRNA
分类号:
R285.5
DOI:
10.19288/j.cnki.issn.1000-2723.2017.02.003
文献标识码:
A
摘要:
目的〓研究肺癌微环境脾气虚证肿瘤组织相关巨噬细胞microRNA的表达及加味黄芪建中汤的影响。方法〓50只小鼠采用苦寒泻下,劳倦,节食的方法造模,在建立小鼠脾气虚证Lewis肺癌模型后,加味黄芪建中汤灌胃治疗10d,脱颈椎处死小鼠,取肿瘤组织。采用microRNA基因芯片检测正常小鼠巨噬细胞与3组肿瘤组织相关巨噬细胞MicroRNA基因表达的变化。结果〓“脾气虚”组肿瘤组织相关巨噬细胞microRNA的表达谱表达异常(与正常组比较),不同于肿瘤组,具有独特的microRNA变化,而加味黄芪建中汤可明显双向调节“脾气虚”组肿瘤组织相关巨噬细胞microRNA的表达谱的异常变化,使其恢复正常。结论〓加味黄芪建中汤可使“脾气虚”组microRNA的表达谱接近恢复正常。
Abstract:
Objective To study the expression of tumor associated macrophages microRNA in lung cancer microenvironment spleen deficiency syndrome and the influence of modified HuangqiJianzhong soup. Methods Using bitter and cold, diarrhea, weary, dieting methods of modeling in 50 mices, after the establishment of mouse spleen deficiency syndrome and Lewis lung cancer model, according to the experimental design of the processing of each group of mice, after modeling Huangqi Jianzhong decoction gavage, the mice were killed off the cervical spine, and tumor tissue was collected. Use MicroRNA gene chip for the detection of variations of normal mice macrophages and three groups of tumor tissue macrophage expression of MicroRNA gene. Results MicroRNA microarray detection of tumor associated macrophages of mice found, Spectrum expression of Spleen Qi deficiency group tumor associated macrophage microRNA(compared with the normal group)aberrant, unlike tumor group, has a unique MicroRNA changes, and modified Huangqi Jianzhong decoction can significantly bidirectional regulate “Spleen Qi Deficiency” abnormal changes in expression profiles of tumor-associated macrophages microRNA tissue to make it back to normal. Conclusion The modified Huangqi Jianzhong decoction can make “Spleen Qi Deficiency” microRNA back to normal.

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备注/Memo

备注/Memo:
* 基金项目: 浙江省自然科学基金(LY13H270010)
收稿日期: 2017 - 02- 27
作者简介: 魏自太(1971-),男,甘肃庄浪人,医学博士,副教授,研究方向:中医药治疗肿瘤疾病。
△通信作者:包素珍,E-mail:weizitai1971@163.com
更新日期/Last Update: 2017-04-30