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|本期目录/Table of Contents|

灯盏乙素对乳腺癌细胞中环状RNA OXNAD1与SMARCA5的影响(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2017年02期
页码:
24-26
栏目:
实验研究
出版日期:
2017-04-30

文章信息/Info

Title:
Effects of Scutellarin on CircRNA OXNAD1 and SMARCA5 of Breast Cancer Cells
作者:
倪广惠12刘加美1赵正波1何越峰3
1. 云南中医学院中药学院,云南 昆明 650500;2. 云南中医学院云南省民族特色养生理论与健康产品工程实验室,云南 昆明 650500;3. 昆明医科大学公共卫生学院,云南 昆明 650500
Author(s):
NI Guanghui12 LIU Jiamei1 ZHAO Zhengbo1 HE Yuefeng3
1. College of Pharmaceutical Science, Yunnan University of Traditional Chinese Medicine, Kunming, 650500, China;2. Engineering Laboratory for National Healthcare Theories and Products of Yunnan Province, Yunnan University of Traditional Chinese Medicine, Kunming, 650500, China; 3. School of Public Health, Kunming Medical University, Kunming, 650500, China
关键词:
灯盏乙素 乳腺癌 circRNA OXNAD1 SMARCA5
Keywords:
scutellarin breast cancer circRNA OXNAD1 SMARCA5
分类号:
R285.5
DOI:
10.19288/j.cnki.issn.1000-2723.2017.02.005
文献标识码:
A
摘要:
目的〓检测灯盏乙素对乳腺癌MDA-MB-231细胞中的环状RNA(Circular RNA,circRNA)的相对表达量的影响,进一步探索灯盏乙素对肿瘤的作用机制。方法〓采用实时荧光定量PCR的方法,检测对照组与不同剂量组的灯盏乙素处理后的乳腺癌细胞中circRNA OXNAD1与SMARCA5的相对表达量。结果〓灯盏乙素在低剂量(1、10、25μm)的情况下,使得乳腺癌MDA-MB-231细胞中circRNA OXNAD1的表达量显著降低(P<0.05),在高剂量(100μm)时显著升高(P<0.05)。灯盏乙素在低剂量(1、10、25、50μm)的情况下,使得乳腺癌MDA-MB-231细胞中环状RNA SMARCA5的表达量显著降低(P<0.05),在高剂量(100μm)时显著升高(P<0.05)。结论〓灯盏乙素影响circRNA OXNAD1与SMARCA5的表达,并且存在剂量反应关系,低剂量与高剂量呈现出相反的表达量变化。
Abstract:
Objective To investigate the effect of scutellarin on the relative expression of circRNA in breast cancer MDA-MB-231 cells, and to further explore the mechanism of scutellarin on tumor. Methods The relative expression of circRNAs OXNAD1 and SMARCA5 in breast cancer cells treated with scutellarin was detected by real-time quantitative PCR in the control group and the different dose groups. Results The relative expression of circRNA OXNAD1 in breast cancer MDA-MB-231 cells was significantly decreased(P<0.05) at low dose(1, 10 and 25μm), and significantly increased at high dose(100μm)(P<0.05). The expression of circRNA SMARCA5 in breast cancer MDA-MB-231 cells was significantly decreased(P<0.05) at the low dose(1, 10, 25, 50μm), and significant at high dose(100μm)(P<0.05). Conclusion Scutellarin has an effect on the expression of circRNA OXNAD 1 and SMARCA5 with a dose-response relationship, the low doses and high dose showed opposite expression changes.

参考文献/References

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备注/Memo

备注/Memo:
* 基金项目: 云南省教育厅科学研究基金项目(2015Y314)
收稿日期: 2017 - 03- 20
作者简介: 倪广惠(1983-),女,江西萍乡人,博士,讲师,主要从事天然产物的有机合成及活性研究。
△通信作者:何越峰,E-mail:heyuefeng@kmmu.edu.cn
更新日期/Last Update: 2017-04-30