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壮通饮对大鼠心肌缺血血瘀证内皮素1表达的影响(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2017年03期
页码:
11-14
栏目:
实验研究
出版日期:
2017-07-17

文章信息/Info

Title:
Effect of Zhuangtongyin on Expression of ET -1 in Rats with Myocardial Ischemia and Blood Stasis Syndrome
作者:
张世田1黄小珊2庞路路2冯悦2唐汉庆1黄岑汉1
1. 右江民族医学院,广西 百色 533000;2. 广西中医药大学,广西 南宁 530200
Author(s):
ZHANG Shitian1HUANG Xiaoshan2PANG Lulu2FENG Yue2TANG Hanqing1HUANG Cenhan1
1. Youjiang Medical University for Nationalities, Baise 533000, China;2. Guangxi University of Chinese Medicine, Nanning 530200, China
关键词:
壮通饮 心肌缺血 血瘀证 内皮素1
Keywords:
Zhuangtongyin myocardial ischemia blood stasis syndrome endothelin 1
分类号:
R285.5
DOI:
10.19288/j.cnki.issn.1000-2723.2017.03.003
文献标识码:
A
摘要:
目的 观察壮通饮对心肌缺血血瘀证大鼠血清及心肌组织内皮素1(endothelin-1,ET-1)表达的影响,探讨壮通饮对冠心病心肌缺血的保护作用机制。方法 采用左冠状动脉结扎法制作大鼠心肌缺血血瘀证模型,并随机分为空白、假手术组、模型组、壮通饮低(6.8g/kg)、中(13.6g/kg)、高(27.2g/kg)剂量组、阳性对照组7个组,灌胃给药治疗4周后,分别采用酶联免疫吸附法(ELISA)和WB(Western blotting)法检测大鼠血清及心肌组织ET-1的表达。结果 血清ELISA结果显示,与空白组、假手术组相比,模型组ET-1的表达明显升高(P<0.05),药物干预后,各组均可使ET-1降低,但中剂量组降低ET-1效果最明显(P<0.05)。WB检测结果显示,模型组ET-1的平均灰度值明显高于空白组、假手术组,药物干预后,各组均可使ET-1平均灰度值降低,但中剂量组降低效果最明显。结论 壮通饮能减少心肌缺血血瘀证大鼠ET-1的表达,缓解血管收缩,对大鼠心肌缺血有一定的保护作用。
Abstract:
Objective To observe the effect of Zhuangtongyin on the expression of ET-1 in serum and myocardium of rats with myocardial ischemia and blood stasis syndrome, and to explore the protective mechanism of Zhuangtongyin on myocardial ischemia in patients with coronary heart disease. Methods Rat model of myocardial ischemic blood stasis was established by left coronary artery ligation. The rats were randomly divided into blank, sham operation group, model group, Zhuangtongyin low dose(6.8g/kg), medium(13.6g/kg)(27.2g/kg)and positive control group 7 groups. After 4 weeks of intragastric administration, the expression of ET-1 in serum and myocardium was detected by enzyme-linked immunosorbent assay(ELISA) and WB(Western blotting). Results Serum ELISA results showed that, Compared with the blank group and the sham operation group, the expression of ET-1 in the model group was significantly increased(P<0.05), after treatment, ET-1 could decrease ET-1, but the effect of ET-1 was lower in middle dose group(P<0.05). WB results showed that, the mean gray value of ET-1 in model group was significantly higher than that in blank group, sham operation group, after the intervention, the average gray value of ET-1 was decreased, but the effect of the middle dose group was the most obvious. Conclusion Zhuangtong can reduce the expression of ET-1 in rats with myocardial ischemia and blood stasis syndrome , alleviate vasoconstriction, and protect the myocardial ischemia in rats.

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备注/Memo

备注/Memo:
* 基金项目: 国家自然科学基金(81460658);广西高校右江流域特色民族药研究重点实验室开放课题(kfkt2016026)
收稿日期: 2017 - 05- 15
作者简介: 张世田(1980-),男,山东潍坊人,在读硕士研究生,研究方向:冠心病中西医结合治疗的基础与临床研究。
△通信作者:唐汉庆,E-mail:phdtanghanqing@163.com
更新日期/Last Update: 2017-06-20