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|本期目录/Table of Contents|

三丫苦茎总提取物及分段样品抗菌活性研究(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2017年03期
页码:
28-32
栏目:
实验研究
出版日期:
2017-07-17

文章信息/Info

Title:
Antibacterial Properties of Total Extract and Ethanol Segments from Stem of Evodia lepta (Spreng. ) Merr. against Common Pathogenic Bacteria in Vivo and in Vitro
作者:
高英师冰颜宏詹易查雨锋张雪梅李续宏△
云南省药物研究所/云南白药集团创新研发中心/云南省中药和民族药新药创制企业重点实验室,云南 昆明 650111
Author(s):
GAO YingSHI BingYAN HongZHAN YiZHA YufengZHANG XuemeiLI Xuhong
Yunnan Institute of Materia Medica, Kunming/Innovation and R&D Center, Yunnan Bai Yao Group, Kunming/Yunnan Province Company Key Laboratory for TCM and Ethnic Drug of New Drug Creation, Kunming 650111, China
关键词:
三丫苦 细菌 小鼠 脓毒症
Keywords:
Evodia lepta (Spreng. ) Merr. bacteria mice sepsis
分类号:
R285.5
DOI:
10.19288/j.cnki.issn.1000-2723.2017.03.007
文献标识码:
A
摘要:
云南省药物研究所/云南白药集团创新研发中心/云南省中药和民族药新药创制企业重点实验室,云南 昆明 650111
Abstract:
Objective Antibacterial properties of total extract and ethanol segments from stem of Evodia lepta(Spreng. ) Merr. in vivo and in vitro. Methods In vitro, minimum inhibitory concentration(MIC) was determined by using the serial 2-fold broth dilution methods. In vivo, a sepsis mouse model challenged with sublethal Staphylococcus aureus was established to evaluate the anti-infective effect of there samples. Results Except the MICs of the total exact for two Staphylococcus aureus strains ATCC29213, ATCC43300 were both 12.5 mg/ml(206.3 mg crude drug/ml), the total exact had no significant inhibition on other pathogenic bacteria such as Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Enterococcus faecalis. In vivo, compared with the model group, the total extract of Evodia lepta(Spreng. ) Merr. significantly increased survival rate of the septic mice within 168 hours(P<0.01). The survival rates were markedly higher in the segments(SYK20, 90)-treated groups than that in the model group(P<0.05; P<0.01). The survival rate was highest in the SYK90, but it was slightly lower than the total exact. Conclusion Taken together, in vitro, the total exact from Evodia lepta(Spreng. ) Merr. significantly inhibits the growth of Staphylococcus aureus. In vivo, the total exact total extract and ethanol segments (SYK20, 90) significantly protects the sepsis mice challenged with sublethal Staphylococcus aureus.

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备注/Memo

备注/Memo:
* 基金项目: 云南省中药和民族药创新重点实验室项目(20151G043)
收稿日期: 2017 - 04- 26
作者简介: 高英(1982-),女,陕西合阳人,助理工程师,研究方向:抗感染药物筛选研究。
△通信作者:李续宏,E-mail:290404711@qq.com
更新日期/Last Update: 2017-06-20