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灯盏益肾颗粒对糖尿病肾病大鼠TGF-β/Smad转导通路的影响(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2017年05期
页码:
6-12
栏目:
实验研究
出版日期:
2017-12-16

文章信息/Info

Title:
Effect of Breviscapine Yishen Granules on TGF-β/Smad Dransduction Pathway in Diabetic Dephropathy Rats
作者:
杜义斌1段艳蕊1王亚东2易欢2谢铭君2
1. 云南中医学院第一附属医院,云南 昆明 650021;2. 云南中医学院,云南 昆明 650500
Author(s):
DU Yibin1 DUAN Yanrui1 WANG Yadong2 YI Huan2 XIE Mingjun2
1. The First Affiliated Hospital of Yunnan University of Traditional Chinese Medicine, Kunming 650021, Chnia;2. Yunnan University of Traditional Chinese Medicine, Kunming 650500, Chnia
关键词:
糖尿病肾病灯盏益肾颗粒实验研究TGF-Smad信号通路
Keywords:
diabetic nephropathy breviscapine Yishen granules experimental study TGF-Smad signaling pathway
分类号:
R285.5
DOI:
10.19288/j.cnki.issn.1000-2723.2017.05.002
文献标识码:
A
摘要:
目的 观察灯盏益肾颗粒对糖尿病肾病(DN)大鼠TGF-Smad转导通路的影响,探讨其对DN防治作用。方法 雄性SD大鼠90只随机分成正常组(A组)、模型组(B组)、盐酸贝那普利组(C组)、灯盏益肾低、中、高剂量组(D、E、F组)。链脲佐菌素(STZ)55mg/kg一次性腹腔注射建立DN模型,给予不同实验因素干预后,生化检测胱抑素C(CysC)、血肌酐(Scr)、血尿素氮(BUN)、尿蛋白/尿肌酐(Up/Ucr);免疫组化法测定大鼠肾组织TGF-β1、Smad 2、Smad 7、Ⅳ型胶原、纤维黏连蛋白(FN)的表达。结果 E、F组Up/Ucr、CysC、BUN低于B组(P<0.05或P<0.01);E、F组肾组织中TGF-β1、Smad 2、Ⅳ型胶原、FN蛋白表达明显低于B组(P<0.01)、低于C、D组(P<0.05),Smad 7蛋白表达多于B、C、D组(P<0.05)。结论 灯盏益肾颗粒可以对糖尿病肾病大鼠肾脏组织中TGF-β/Smad信号传导通路产生影响,调控肾组织中Ⅳ型胶原、FN表达,影响肾纤维化进程,随剂量增加,影响作用增强。
Abstract:
Objective To observe the effect of breviscapine Yishen Granule on TGF-Smad transduction pathway in diabetic nephropathy(DN) rats, and to explore its preventive and therapeutic effects on DN. Methods The 90 male SD rats were randomly divided into normal group(group A), model group(group B), hydrochloric acid Benner Pury group(group C), and the Breviscapine Yishen granules’s group were divided into low, middle and high dose group(group C, D, E). While, with streptozotocin(STZ)intraperitoneal injection of 55mg/kg to set up DN model. After given different experimental factors intervention, observed The levels of cystatin C(CysC), serum creatinine(Scr), blood urea nitrogen(BUN) and urinary protein/urine creatinine(Up/Ucr) by biochemical detection. And observed the expression of TGF-beta 1, Smad 2, Smad 7, collagen type IV and fibronectin(FN) in renal tissue of rat models was detected by immunohistochemistry. Results The levels of of E, F Up/Ucr, Cys C, BUN group was lower than that of B group(P<0.05 or P<0.01); The expression of TGF-beta 1, Smad 2, type IV collagen, FN protein in group E and F renal tissue was significantly lower than that of group B(P<0.01), lower than group C and D(P<0.05), the expression of Smad 7 protein was higher than group B(P<0.05), more than group C and D(P<0.05). Conclusion Breviscapine Yishen granule can inhibit the activation of TGF-Smad signaling pathway in renal tissue of diabetic nephropathy rats, and reduce the expressions of type IV collagen and FN protein in tissues. The effect of medium and high dose group was better than that of low dose group and Benner Pury hydrochloride group.

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备注/Memo

备注/Memo:
* 基金项目: 云南省科技计划面上项目(2014FZ105)
收稿日期: 2017 - 10- 18
作者简介: 杜义斌(1968-),男,云南梁河人,医学硕士,主任医师,研究方向:老年肾脏病中西医结合治疗。
△通信作者: 段艳蕊,E-mail:ddyyrr97@163.com
更新日期/Last Update: 2017-10-20