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电针预处理对脂多糖诱导的急性肺损伤大鼠炎性细胞因子及水通道蛋白5的影响(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2017年05期
页码:
13-17,22
栏目:
实验研究
出版日期:
2017-12-16

文章信息/Info

Title:
Effect of Electroacupuncture Pretreatment on Inflammatory Cytokines and Aquaporin-5in Acute Lung Injury Rats Induced by Lipopolysaccharide
作者:
黄文彪1张新芳1刘自兵2卞海1舒琦2李庚儒1李肖晴1刘小云2陶青青1
1. 安徽中医药大学中西医结合学院,安徽 合肥 230038;2. 安徽中医药大学针灸推拿学院,安徽 合肥 230038
Author(s):
HUANG Wenbiao1 ZHANG Xinfang1 LIU Zibing2 BIAN Hai1 SHU Qi2 LI Gengru1 LI Xiaoqing1 LIU Xiaoyun2 TAO Qingqing1
1. College of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei 230038, China;2. College of Acupuncture and Massage, Anhui University of Chinese Medicine, Hefei 230038, China
关键词:
急性肺损伤水通道蛋白5电针预处理足三里IL-1βIL-6IL-10TNF-α
Keywords:
acute lung injury aquaporin-5(AQP5) electroacupuncture pretreatment Zusanli IL-1β IL-6 IL-10 TNF-α
分类号:
R245.9+7
DOI:
10.19288/j.cnki.issn.1000-2723.2017.05.003
文献标识码:
A
摘要:
目的 探讨电针足三里穴预处理对脂多糖(LPS)诱导的急性肺损伤(ALI)大鼠的干预作用及可能机制。方法 将SD大鼠随机分为正常、ALI4h、ALI6h、针刺+ALI4h和针刺+ALI6h组,ALI4h、ALI6h组大鼠气管内用LPS(2mg/kg)分别滴注4h和6h以诱导ALI,后两组大鼠在针刺预处理双侧足三里穴1周后于气管内分别滴注LPS4h和6h。各组均取血、支气管肺泡灌洗液(BALF)及肺组织,计算BALF中白细胞总数及肺湿/干比(W/D),HE染色观察肺组织形态,ELISA方法检测血浆中AQP5、IL-1β、IL-6、IL-10、TNF-α和BALF中AQP5水平,免疫组化方法观察肺组织AQP5表达。结果 与正常组比较,ALI4h组白细胞计数、W/D增高(P<0.05)、肺泡总面积和肺组织面积之比(A/t)降低(P<0.05)以及IL-1β、IL-10、TNF-α含量显著增高(P<0.001);ALI6h组IL-6和IL-10含量明显增高(P<0.01);ALI4h、ALI6h组AQP5含量显著降低(P<0.001)、积分光密度(IOD)明显降低(P<0.01)。与ALI4h组比较,针刺+ALI4h组白细胞计数与W/D降低(P<0.05)、IL-1β与TNF-α含量降低(P<0.05)、血浆及BALF中AQP5含量均升高(P<0.05),针刺+ALI6h组血浆AQP5含量有升高(P<0.05)。结论 电针足三里穴预处理对LPS诱导的ALI大鼠具有抗炎、降低肺水肿的保护作用,且在诱导4h时的ALI中更佳,其机制可能与电针下调炎性细胞因子和上调AQP5的表达有关。
Abstract:
Objective To investigate the influence of the pretreatment of electroacupuncture(EA) on the inflammatory cytokines and aquaporin(AQP)-5 in acute lung Injury rats induced by lipopolysaccharide. Methods SD-rats were randomly divided into 5 groups:control, ALI4h, ALI6h, EA plusALI4h and EA plus ALI6h. Acute lung injury was induced by LPS(2mg/kg) stilling into the trachea respectively for 4h and 6h except the normal group. The rats in latter two groups were pretreated with EA at bilateral Zusanli(ST36)points for a week. The plasma was separated for detecting the content of AQP5, IL-1β, IL-6, IL-10 and TNF-αby enzyme-linked immuno sorbent assay(ELISA) method. Bronchoalveolar lavage fluid(BALF) of rats was extracted for the content of AQP5 by ELISA and counting the total number of white blood cells(WBC). The left lung tissue was used for the ratio of wet to dry weight(W/D), and right lung was fixed in formaldehyde for observation of morphology and immunohistochemistry. Results Compared with the control group, in ALI4h group W/D and WBC count was increased(P<0.05), the ratio of total area of alveolar to lung tissue area(A/t) was decreased(P<0.05), IL-10, TNF-α and IL-1β were significantly increased(P<0.001); and IL-6 and IL-10 were significantly higher(P<0.01) in ALI6h group; the content of AQP5 decreased significantly(P<0.001), integral optical density(IOD) decreased significantly(P<0.01) in ALI4h and ALI6h groups. Compared with ALI4h group, WBC count and W/D decreased(P<0.05), IL-1β and TNF-α content decreased(P<0.05), AQP5 content in plasma and BALF increased(P<0.05), and plasma AQP5 content in acupuncture group was increased in EA plus ALI4h group. Conclusion EA pretreatment at Zusanli could protect ALI rats induced LPS from inflammatory and reduce pulmonary edema, and the effect was better in ALI4h group than in ALI6h group. The mechanism may be related to the reduction of inflammatory cytokines and the up-regulation of AQP5 expression by EA pretreatment.

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备注/Memo

备注/Memo:
* 基金项目: 国家自然科学基金(81102660,81373743);安徽省大学生创新创业项目(2014067)
收稿日期: 2017 - 10- 22
作者简介: 黄文彪(1995-),男,安徽六安人,在读本科生,研究方向:针刺作用机制。
△通信作者: 张新芳,E-mail:xfzhang2009@126.com
更新日期/Last Update: 2017-10-20