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白芍总苷胶囊对自身免疫性甲状腺炎模型大鼠Treg细胞的影响*(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2017年06期
页码:
5-8
栏目:
实验研究
出版日期:
2018-03-19

文章信息/Info

Title:
Effect of Total Glucosides of Paeony Capsules on Treg Cells in AIT Model Rats
作者:
余欣然1向楠2李家园1李章青1
1. 湖北中医药大学,湖北 武汉 430065;2. 湖北中医药大学第一临床学院,湖北 武汉 430061
Author(s):
YU Xinran1 XIANG Nan2 LI Jiayuan1 LI Zhangqing1
1. Hubei University of Traditional Chinese Medicine, Wuhan 430065, China;2. The First Clinical College, Hubei University of Traditional Chinese Medicine, Wuhan 430061, China
关键词:
白芍总苷 自身免疫性甲状腺炎 大鼠 调节性T细胞
Keywords:
total glucoside of Paeonia lactiflora autoimmune thyroiditis rat regulatory T cells
分类号:
R285.5
DOI:
10.19288/j.cnki.issn.1000-2723.2017.06.002
文献标识码:
A
摘要:
目的 研究白芍总苷胶囊对于自身免疫性甲状腺炎(AIT)模型大鼠调节性T细胞(Treg)的影响。方法 40只4~5周SD雌性大鼠为研究对象,按照随机数表法分为空白对照组、模型组、白芍总苷组、硒酵母组,每组各10只,模型组、白芍总苷组、硒酵母予以皮下注射猪甲状腺球蛋白(pTg),并配合高碘水喂养制造AIT模型,同时白芍总苷组、硒酵母组分别予以白芍总苷、硒酵母生理盐水溶液灌胃,6周后处死大鼠,酶联免疫吸附法(ELISA)检测大鼠血清促甲状腺激素(TSH)、甲状腺球蛋白抗体(TGAb)、甲状腺过氧化物酶抗体(TPOAb)水平,流式细胞仪检测大鼠脾脏CD4+CD25+Foxp3+Treg细胞比例。结果 模型组大鼠TSH、TGAb、TPOAb水平较空白组显著升高,白芍总苷、硒酵母组低于模型组,且白芍总苷组各指标均低于硒酵母组,差异具有统计学意义(P<0.05);模型组大鼠脾脏CD4+CD25+Foxp3+Treg比例低于空白对照组,白芍总苷、硒酵母组高于模型组,差异具有统计学意义(P<0.05),白芍总苷组与硒酵母组对比差异无统计学意义(P>0.05)。结论 白芍总苷胶囊可降低AIT大鼠TSH、从TGAb、TPOAb水平,改善免疫失衡状态,可能与其提高CD4+CD25+Foxp3+Treg比例的功能相关。
Abstract:
Objective To study and explore the effect of Total Glucosides of Paeony Capsules on the regulatory T cells(Treg) of autoimmune thyroiditis(AIT) model rats. Methods Forty SD rats aged 4~5 weeks were divided into blank control group, model group, total glucosides of paeony, selenium yeast group, 10 rats in each group, model group, white group(PTg) was injected subcutaneously into the pituitary gland glycosides group and selenium yeast, and AIT model was induced by high iodine water feeding. At the same time, the total glucosides of paeoniflorin group and the selenium yeast group were respectively treated with total glucosides of paeony and selenium yeast physiological saline solution After 6 weeks, the rats were sacrificed and the levels of serum TSH, TGAb and TPOAb were measured by enzyme-linked immunosorbent assay(ELISA) Proposed according to the acronym requirements specification, flow cytometry rat spleen CD4+CD25+Foxp3+Treg cell ratio. Results The levels of TSH, TGAb and TPOAb in model group were significantly higher than those in blank group. The total glucosinolate and selenium in paeony were lower than those in model group, and the indexes in paeoniflorin were lower than those in selenium group(P<0.05). The proportion of CD4+CD25+Foxp3+Treg in spleen of rats in model group was lower than that of blank control group, the total glucosinolate and selenium of paeony was higher than that of model group(P<0.05) There was no significant difference between the groups of total glucosides of paeony and selenium(P>0.05). Conclusion Total Glucosides of Paeony Capsules can reduce the level of TSH, TGAb and TPOAb in AIT rats, and improve the immune imbalance, which may be related to the function of increasing the proportion of CD4+CD25+Foxp3+Treg.

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备注/Memo

备注/Memo:
* 基金项目: 国家中医药管理局第一批全国中医学术流派传承工作室建设项目(国中医药办人教函[2012]170)
收稿日期: 2017 - 11- 18
作者简介: 余欣然(1989-),女,湖北广水人,在读博士研究生,研究方向:中医药防治内分泌和代谢性疾病。
△通信作者: 向楠,E-mail:hbzyydxbs@163.com
更新日期/Last Update: 2017-12-20