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钩藤碱对LPS诱导的多巴胺能神经元及胶质细胞炎症及TNF-α和IL-1β影响(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2018年02期
页码:
6-10
栏目:
实验研究
出版日期:
2018-11-10

文章信息/Info

Title:
Rhynchophylline Effects on Inflammation and Related Inflammatory Factor of TNF-α and IL-1β of Dopaminergic Neurons and Glial Cells Induced by Lipopolysaccharide
作者:
张婷12李继君1△孙克兴1刘忠3Xiaohong Li1
1. 上海交通大学医学院附属上海儿童医学中心中医科,上海 200127; 2. 浙江中医药大学药学院,浙江 杭州 310053;3. 上海交通大学药学院,上海 200240
Author(s):
ZHANG Ting12 LI Jijun1 SUN Kexing1 LIU Zhong3 Xiaohong Li1
1. Department of Integrative Medicine on Pediatrics, Shanghai Children’s Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China; 2. School of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 310053, China; 3. School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China
关键词:
钩藤碱 脂多糖 多巴胺能神经元 胶质细胞 炎性因子
Keywords:
rhynchophylline lipopolysaccharide dopaminergic neurons glial cells inflammatory factor
分类号:
R285.5
DOI:
10.19288/j.cnki.issn.1000-2723.2018.02.002
文献标识码:
A
摘要:
目的观察钩藤碱对脂多糖(LPS)诱导的多巴胺能神经元及胶质细胞产生的炎症的影响。方法体外培养胎鼠神经元及神经胶质细胞,通过免疫细胞荧光染色鉴定后,分为4组:空白对照组、LPS模型组、LPS+钩藤碱组、LPS+宁动颗粒组,在干预后0、1、3、6、12 h收集细胞培养液,并ELASA法检测细胞培养液中炎性因子TNF-α,IL-1β的含量。结果LPS介导的炎性组中,在时间上3、6 h作用TNF-α,IL-1β的含量达到高峰;TNF-α,IL-1β浓度,与LPS模型组比较,空白对照组、LPS+钩藤碱组与LPS+宁动颗粒组培养液中IL-1β含量均显著降低(P<0.05),且LPS+钩藤碱组与LPS+宁动颗粒组间无显著性差异。结论钩藤碱可以显著抑制LPS诱导的神经元及神经胶质细胞的炎性因子TNF-α,IL-1β,起到抗炎而保护神经元作用。
Abstract:
Objective To observe the influence of rhynchophylline(Rh) on inflammation and related inflammatory factor of TNF-α and IL-1β in dopaminergic neurons and glial cells induced by lipopolysaccharide(LPS). Methods Dopaminergic neurons and glial cells were extracted, eparated and cultured in 18-day pregnant Wistar rats, and is divided into four groups:control group, LPS model group, the LPS + Rhy group and LPS + NDG group, each group respectively in processing after the completion of 0, 1, 3, 6, 12 h collecting cells and cell culture medium after treated. After immunofluorescence staining, the content of TNF-α and IL-1β in cell culture medium was detected by ELASA. Results The content of the IL-1β and TNF-α went content peak from 3 and 6 hours after treating. Compared with LPS model group, the content of IL-1β and TNF-α were significantly lower in LPS + Rhy group and LPS + NDG(P<0.01), but there was no statistically significant difference between the group of the LPS + Rh and the LPS + NDG. Conclusion Rh can effectively suppress the content of IL-1β and TNF-α, and then to inhibit the inflammatory and protect dopaminergic neurons.

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备注/Memo

备注/Memo:
收稿日期: 2018 - 04- 12 * 基金项目: 国家自然科学基金(81528023,81202724);上海交通大学医工交叉项目(YG2015MS35) 第一作者简介: 张婷(1993-),女,在读硕士研究生,专业方向:中药药理与新产品开发。 通信作者: 李继君,E-mail:lijijun523@hotmail.com
更新日期/Last Update: 2018-04-28