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疏肝理气、化湿活血法对胰岛素抵抗3T3-L1细胞FGF-21水平及其受体表达的影响(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2015年01期
页码:
17-22
栏目:
实验研究
出版日期:
2015-02-10

文章信息/Info

Title:
Effects of “Soothing Liver-Qi, Resolving Dampness and Blood Stasis” on FGF-21 andFGFRs Levels in Insulin-Resistant 3T3-L1 Adipocytes
作者:
龙艳1孙璐2范冠杰2
1.广州市中西医结合医院,广东广州510080;2.广东省中医院,广东广州510120
Author(s):
LONG Yan1 SUN Lu2 FAN Guan-jie2
1. Guangzhou Hospital of integrated Chinese and Western Medicine, Guangzhou 510080, China;2. Guangdong Hospital of Chinese Medicine, Guangzhou 510120, China
关键词:
疏肝理气化湿活血胰岛素抵抗3T3-L1脂肪细胞FGF-21
Keywords:
Soothing Liver-Qi Resolving Dampness and Blood Stasis Insulin-resistant 3T3-L1 adipocytes FGF-21
分类号:
R284
DOI:
-
文献标识码:
A
摘要:
目的观察疏肝理气、化湿活血法对胰岛素抵抗3T3-L1脂肪细胞FGF-21水平及其受体FGFR1及FGFR2表达的影响。方法采用1μmol/L地塞米松诱导脂肪细胞胰岛素抵抗。将细胞分为正常对照组、模型对照组、吡格列酮组及中药高、低剂量组。加入含药血清处理后,分别于24h、48h采用葡萄糖氧化酶法检测各孔细胞上清液葡萄糖浓度,采用酶联免疫吸附法检测上清液中FGF-21浓度,培养后48h采用荧光定量PCR法检测细胞FGFR1及FGFR2基因表达水平。结果中药高、低剂量及吡格列酮均可增加胰岛素抵抗3T3-L1脂肪细胞的葡萄糖消耗量,同时降低其FGF-21水平,与相同时间段模型对照组比较均具有统计学意义(P<0.01);吡格列酮可上调胰岛素抵抗3T3-L1脂肪细胞的FGFR1、FGFR2mRNA表达量(与模型对照组比较P<0.05或P<0.01),中药仅高剂量组可上调其FGFR2mRNA相对表达量(与模型对照组比较P<0.05)。结论疏肝理气、化湿活血法可改善3T3-L1脂肪细胞胰岛素抵抗水平,其机制可能与FGF信号通路有关。
Abstract:
Objective To investigate the effects of “soothing Liver-Qi, resolving dampness and blood stasis” on FGF-21 and FGFR1 and FGFR2 levels in 3T3-L1 adipocytes. Methods Insulin-resistant model of 3T3-L1 adipocytes were induced by 1μmol/L dexamethasone。Then divided into 5 groups: normal control group, model control group Pioglitazone group and Chinese herb with high and low-dose groups. After 24h and 48h cultured with medicated serum, glucose concentration in the culture medium was checked with Glucose Oxidase Method, FGF-21 levels were tested by Enzyme Linked Immunosorbent Assay, and FGFR1/FGFR2 gene expression was measured with Fluorescence Quantitative PCR Method after culturing 48h. Results Glucose consumption was improved, and FGF-21 level was reduced, in insulin-resistant 3T3-L1 adipocytes by Chinese herbs and Pioglitazone (compared with model control group P<0.01); FGFR1 and FGFR2 mRNA expression in insulin-resistant 3T3-L1 adipocytes were both up-regulated by Pioglitazone(compared with model control group P<0.05 or P<0.01), only FGFR2 mRNA expression was up-regulated by high-dose Chinese herbs with a efficiency of “soothing Liver-Qi, resolving dampness and blood stasis”. Conclusion The therapy of “soothing Liver-Qi, resolving dampness and blood stasis” can alleviate insulin-resistant in 3T3-L1 adipocytes, its mechanism may relate to FGF signal pathway.

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备注/Memo

备注/Memo:
收稿日期:2014-10-09作者简介:龙艳(1980-),女,湖南茶陵人,主治中医师,主要从事中医药防治糖尿病的临床及科研工作。E-mail:longyan0823@163.com
更新日期/Last Update: 2015-01-30