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人参皂苷Rg3对兔耳增生性瘢痕TNF-α和IL-β表达的影响(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2016年01期
页码:
6-9
栏目:
实验研究
出版日期:
2016-02-02

文章信息/Info

Title:
Effect of Ginsenoside-Rg3 on L-1β and TNF-α Expression in Hypertrophic ScarFormation in Rabbit Ears Model
文章编号:
1000 - 2723(2016)01 - 0006 - 04
作者:
马莉1李晓静2
1. 安徽中医药大学护理学院医学美容教研室,安徽 合肥 230001;
2. 安徽医科大学第一附属医院整形外科,安徽 合肥 230022
Author(s):
MA Li1 LI Xiaojing2
1. Department of Medical Cosmetology, Anhui University of Traditional Chinese Medicine, Hefei 230001, China;
2. Dept of Plastic Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China
关键词:
病理性瘢痕 白兔 炎症 人参皂苷Rg3 IL-1β TNF-α
Keywords:
hypertrophic scar rabbit inflammation Ginsenoside-Rg3 IL-1β TNF-α
分类号:
R285.5
DOI:
10.19288/j.cnki.issn.1000-2723.2016.01.002
文献标识码:
A
摘要:
目的 观察人参皂苷Rg3对兔耳病理性瘢痕组织中TNF-α和IL-1β表达的影响。方法 建立兔耳病理性瘢痕模型,随机分为治疗组和对照组;各组均用微量注射器行于创面行局部封闭注射,治疗组注射人参皂苷Rg3,对照组注射等量的PBS;通过比较各组瘢痕指数(SEI)及I、III型胶原表达不同评价瘢痕增生程度的差异;采用免疫组化方法检测炎症因子TNF-α和IL-1β的表达。结果 与PBS对照组相比较,人参皂苷Rg3治疗组兔耳病理性瘢痕组织SEI及I、III型胶原沉积显著下降,炎症因子TNF-α和IL-1β表达减少,差异具有统计学意义(P<0.05)。结论 人参皂苷Rg3在创面愈合早期及时干预,可抑制兔耳增生性瘢痕组织中炎症因子TNF-α和IL-1β的表达,减少瘢痕的形成,提示其抗瘢痕作用可能与抑制炎症反应有关。
Abstract:
Objective To observe the effect of Ginsenoside-Rg3 on the expression of interleukin-1β(IL-1β) and tumornecrosis factor-α(TNF-α) in the ear wounds. Methods Collagen I and III expression detected by immunohistochemistry and scar elevation index(SEI) was used to evaluate the extent of scarring. The expression of inflammatory factors interleukin-1β(IL-1β) and tumor necrosis factor-α(TNF-α) was detected by immunohistochemistry. Results Compared with control scars, Ginsenoside-Rg3-treated wounds exhibited decreased inflammation significantly as evidenced by the lower levels of IL-1β and TNF-α. The SEI and the synthesis of collagens I and III were significantly decreased in the Ginsenoside-Rg3-treated scars(P<0.05). Conclusion Immediate topical injection of Ginsenoside-Rg3 during the wound healing process can reduce the severity of hypertrophic scarring in a rabbit model, which may result from controlling inflammation, inducing fibroblast apoptosis and promoting collagen degradation by decreased the inflammatory factors of IL-1β and TNF-α.

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备注/Memo

备注/Memo:
基金项目: 安徽中医药大学校青年基金(2014qn013)
作者简介: 马莉(1982-),女,安徽合肥人,讲师,研究方向:瘢痕的临床和基础研究。E-mail:284305993@qq.com
更新日期/Last Update: 2016-02-28