我们的网站为什么显示成这样?

可能因为您的浏览器不支持样式,您可以更新您的浏览器到最新版本,以获取对此功能的支持,访问下面的网站,获取关于浏览器的信息:

|本期目录/Table of Contents|

三七总皂苷对拟缺血损伤脑微血管内皮细胞转化生长因子-β、血管内皮生长因子表达的影响(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2016年02期
页码:
1-4
栏目:
实验研究
出版日期:
2016-04-10

文章信息/Info

Title:
Effects of Panax Notoginseng Saponins on the Expression of TGF-β and VEGF from Cultured Brain Microvascular Endothelial Cells on the Hypoxia Induced Damage in Vitro
作者:
臧妍妍1李卫红1郭晓谨2万亮琴1李芳赫1张赛1马家宝1姜昭妍1胡艳红1张凡1
1. 北京中医药大学,北京 100029;2. 通州中西医结合医院,北京 101100
Author(s):
ZANG Yanyan1 LI Weihong1 GUO Xiaojin2 WAN Liangqin1 LI Fanghe1 ZHANG Sai1MA Jiabao1 JIANG Zhaoyan1 HU Yanhong1 ZHANG Fan1
1. Beijing University of Chinese Medicine, Beijing 100029, China; 2. Tongzhou Hospital of Integrated Chinese and Western Medicine, Beijing 101100, China
关键词:
三七总皂苷 脑微血管内皮细胞 转化生长因子β1 血管内皮生长因子 缺血损伤 细胞凋亡 血管新生
Keywords:
panax notoginseng saponins(PNS) brain microvascular endothelial cells transforming growth factor-β(TGF-β) vascular endothelial growth factor(VEGF) ischemic injury apoptosis angiogenesis
分类号:
R285.5
DOI:
10.19288/j.cnki.issn.1000-2723.2016.02.001
文献标识码:
A
摘要:
目的观察三七总皂苷对拟缺血损伤脑微血管内皮细胞的凋亡和转化生长因子-β(transforming growth factor-β,TGF-β)、血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响,以期揭示三七总皂苷发挥脑保护作用的分子机制。方法原代培养大鼠脑微血管内皮细胞,采用氧糖剥夺法制备拟缺血损伤模型,分为正常组、模型组、三七总皂苷组,流式细胞术检测细胞凋亡情况、酶联免疫吸附法检测细胞TGF-β1,VEGF-B水平、Western blotting法检测细胞TGF-β蛋白的表达水平。结果模型组细胞凋亡率与正常组相比显著性升高(P<0.01);与模型组相比,三七总皂苷组细胞凋亡率明显下降(P<0.01);与正常组相比,模型组细胞上清液中TGF-β1含量明显增多(P<0.05);与正常组相比,模型组细胞上清液中VEGF-B含量显著性增多(P<0.01);与模型组相比,三七总皂苷组中细胞上清液中VEGF-B含量明显增多(P>0.05);正常组内皮细胞TGF-β表达量较少,造模后,TGF-β表达显著增加(P<0.05),三七总皂苷能够下调TGF-β蛋白的表达。结论三七总皂苷可能通过抑制内皮细胞的凋亡,保持内皮细胞的完整性,上调VEGF表达,促进缺氧条件下血管的新生,这可能是三七总皂苷脑保护的机制之一。
Abstract:
Objective To investigate the effects of the panax notoginseng sapoins(PNS) on brain vascular endothelial cells apoptosis and expression of TGF-β、 VEGF, in order to reveal the molecular mechanism of PNS on brain protection. Methods The model of hypoxia induced damage in cultured brain vascular endothelicocyte cells was set up in vitro and divided into normal group, model group and PNS group. Flow cytometry was adopted to detect cell apoptosis, ELISA was used to determine the protein level of TGF-β1, VEGF-B, the expression of TGF-βwas measured by western blot. Results Compared with the normal group, the rate of apoptosis in model group increased significantly(P<0.01); Compared with the modell group, the rate of apoptosis in PNS group decreased significantly(P<0.01). Compared with the normal group, the level of TGF-β1 increased significantly in model group(P<0.05). In model group the level of VEGF-B increase significantly(P<0.01) compared with the normal group; compared with the model group, the level of VEGF-B in PNS group increased(P>0.05); the expression of TGF-βis less in normal group, expression of TGF-β in model group increased(P<0.05), compared with model group, the expression of TGF-β is decreased. Conclusion PNS can decrease the rate of brain vascular endothelial cells apoptosis, and inpaired the damage of cells, beneficial to angiogenesis, so as to protect the neurons in hypoxia injury.

参考文献/References

[1] Arai K,Jin G,Navaratna D,et al. Brain angiogenesis in developmental and pathological processes:neurovascular injury and angiogenic recovery after stroke[J]. FEBS J,2009,276(17):4644-4652.
[2] 常坤鹏,丁小灵. 缺血性脑卒中新生血管相关因子的研究[J]. 脑与神经疾病杂志,2015,23(4):312-316.
[3] Otrock ZK,Makarem JA,Shamseddine AI. Vascular endothelial growth factor family of ligands and receptors:review[J]. Blood Cells Mol Dis,2007,38(3):258-268.
[4] 李卫红,李澎涛,华茜,等. 不同内皮细胞条件培养液对皮层神经元线粒体功能的影响及通络救脑注射液的保护作用[J]. 中国中西医结合杂志,2007,27(2):131-134.
[5] 朱元,李澎涛,李卫红,等. 不同状态的大鼠星形胶质细胞条件培养液对脑微血管内皮细胞血脑屏障特征性酶的影响[J]. 世界科学技术——中医药现代化,2012,14(2):1393-1398.
[6] Kim KS. Microbial translocation of the blood–brain barrier[J]. Int J Parasitol,2006,36(5):607-614.
[7] 万赛英,顾卫,谭锋. 转化生长因子 β1 与脑缺血[J]. 国外医学:脑血管疾病分册,2005,13(8):591-595.
[8] 万赛英,黎杏群,智屹惠,等. 脑微血管内皮细胞缺氧模型转化生长因子 β1 表达与脑溢安的干预[J]. 中国组织工程研究,2011,15(46):8625-8629.
[9] 雍海溟,宋强,张水华. 血管内皮生长因子 B 生物学功能的研究进展[J]. 医学研究生学报,2012,25(7):774-777.
[10] Sun Y,Jin K,Childs JT,et al. Vascular endothelial growth factor-B(VEGFB)stimulates neurogenesis:evidence from knockout mice and growth factor administration[J]. Dev Biol,2006,289(2):329-335.
[11] 徐双云,徐家丽,沈怀云,等. 缺氧缺血性脑病新生儿血浆中血管内皮生长因子动态变化的临床意义[J]. 蚌埠医学院学报,2009,34(8):669-671.
[12] 徐冉,段淑荣,王慧慧,等. 人脑梗死后转化生长因子-β 及血管内皮生长因子的表达及神经保护作用研究[J]. 中国全科医学,2010,13(23):2560-2563.
[13] Shih SC,Ju M,Liu N,et al. Transforming growth factor β1 induction of vascular endothelial growth factor receptor 1:mechanism of pericyte-induced vascular survival in vivo[J]. Proc Natl Acad Sci U S A,2003,100(26):15859-15864.

备注/Memo

备注/Memo:
* 基金项目: 国家自然科学基金(81273885);北京中医药大学“航海中医药”协同创新项目(522/0100604299) 收稿日期: 2016 - 03 - 01 作者简介: 臧妍妍(1986-),女,河南周口人,在读硕士研究生,研究方向:中药复方的配伍机制。△通信作者:李卫红,E-mail:liweihong.403@163.com
更新日期/Last Update: 2016-03-20