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一测多评法测定百蕊草中百蕊草素Ⅰ、百蕊草素Ⅱ和山奈酚的含量(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2016年02期
页码:
30-33
栏目:
方药研究
出版日期:
2016-04-10

文章信息/Info

Title:
Simultaneous Assay of Kaempferol-3-O-glucorhamnoside, Astragalin and Kaempferol andin Thesium by Quantitative Analysis of Multi-components by Single Marker
作者:
储晓琴1蒋建勤1徐江慧2洪晓琴2
1. 中国药科大学天然药物化学教研室,江苏 南京 211198;2. 安徽中医药大学,安徽 合肥 230012
Author(s):
CHU Xiaoqin1 JIANG Jianqin1 XU Jianghui2 HONG Xiaoqin2
1. Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 211198, China;2. School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China
关键词:
一测多评 百蕊草 相对较正因子 山奈酚 百蕊草素Ⅰ 百蕊草素Ⅱ
Keywords:
quantitative analysis of multi-components by single marker(QAMS) Thesium the calibration factor kaempferol kaempferol-3-O-glucorhamnoside Astragalin
分类号:
R284
DOI:
10.19288/j.cnki.issn.1000-2723.2016.02.008
文献标识码:
A
摘要:
目的建立百蕊草中百蕊草素Ⅰ、百蕊草素Ⅱ与山奈酚的一测多评含量测定方法。方法采用C18色谱柱(phenomenex,250×4.6mm,5μm);以甲醇-0.4%磷酸溶液为流动相;梯度洗脱(0→5→10→20→45min,45%→50%→55%→60%甲醇);波长365nm;流速1.0mL/min;柱温40℃。以山奈酚为参照物,建立其与百蕊草素Ⅰ和百蕊草素Ⅱ的相对校正因子,并计算其含量,实现一测多评。同时采用外标法测定3个成分的含量,并比较二者差异,以验证一测多评法的准确性和可行性。结果采用一测多评法和外标法测定的含量间无显著差异,RSD<2%. 结论本文建立的一测多评法控制百蕊草的质量可行、准确。
Abstract:
Objective To develop a method of quantitative analysis of multi-components by single marker(QAMS) for simultaneously determining kaempferol-3-O-glucorhamnoside, astragalin and kaempferol in Thesium. Methods The chromatographic separation was achieved on C18 chromatographic column(phenomenex, 250×4.6mm, 5μm), the mobile phase was methanol -0.4% phosphoric acid aqueous solution with gradient elution(0→5→10→20→45min, 45%→50%→55%→60% methanol). The wavelength was 365nm. The flow rate was 1. 0 mL/min and the column temperature was 40℃. Kaempferol was used as the reference, the calibration factor of kaempferol-3-O-glucorhamnoside and Astragalin to that of kaempferol were calculated. And the contents of active constituents were calculated by the relative calibration factor to realize QAMS. At the same time, external standard method was used to detect the contents of the three constituents, the two methods were compared to validate the accuracy and feasibility of the new method. Results No significant differences were found in the quantitative results between the measured value and the calculated value. Conclusion The QAMS is feasible and accurate to evaluate the contents of the three active constituents in Thesium.

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备注/Memo

备注/Memo:
* 基金项目: 安徽省自然科学基金(1408085QH183) 收稿日期: 2015 - 11 - 12 作者简介: 储晓琴(1991-),女,在读博士研究生,副教授,研究方向:中药分离、剂型设计及质量控制。△通信作者:蒋建勤,E-mail:cpujq@aliyun.com
更新日期/Last Update: 2016-03-20