我们的网站为什么显示成这样?

可能因为您的浏览器不支持样式,您可以更新您的浏览器到最新版本,以获取对此功能的支持,访问下面的网站,获取关于浏览器的信息:

|本期目录/Table of Contents|

除湿通痹方对大鼠急性痛风性关节炎抗炎作用的实验研究(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2016年03期
页码:
10-13
栏目:
实验研究
出版日期:
2016-05-15

文章信息/Info

Title:
Experimental Study on the Anti-inflammatory Effects in Rats with Acute Gouty Arthritis of the Gout One Prescription
作者:
王涛1廖江龙1艾元亮1许燕飞1邱发敏2徐平2温辉2黄文泽2李雷1
1. 昆明市中医医院,云南 昆明 650011;2. 云南中医学院,云南 昆明 650500
Author(s):
WANG Tao1 LIAO Jianglong1 AI Yuanliang1 XU Yanfei1 QIU Famin2 XU Ping2 WEN Hui2 HUANG Wenze2 LI Lei1
1. Kunming City Hospital of Tradition Chinese Medicine, Kunming 650011, China;2. Yunnan University of Traditional Chinese Medicine, Kunming 650500, China
关键词:
除湿通痹方 急性痛风性关节炎 尿酸钠 TNF-α IL-1β
Keywords:
gout one prescription acute gouty arthritis monosodiumurate TNF-α IL-1β
分类号:
R285.5
DOI:
10.19288/j.cnki.issn.1000-2723.2016.03.003
文献标识码:
A
摘要:
目的研究除湿通痹方对尿酸钠(MSU)诱导的急性痛风性关节炎模型大鼠足关节肿胀率及足关节滑膜细胞因子表达的影响,探讨该方治疗急性痛风性关节炎的部分作用机制。方法选用Wistar雄性大鼠40只,随机分成生理盐水组、阳性对照组、除湿通痹方I组、Ⅱ组。各组给予相应药物灌胃,2次/d,第5天灌胃1.5h后予尿酸钠晶体注射局部关节腔形成大鼠急性痛风性关节炎模型,并于不同时间测量大鼠足关节肿胀度,观察该方对大鼠足关节肿胀率的影响;注射尿酸钠24h后,处死大鼠并应用RT-PCR,检测对关节滑膜细胞因子TNF-α mRNA、IL-1βmRNA表达的影响。结果除湿通痹方可有效抑制大鼠踝关节肿胀程度;与生理盐水组比较,TNF-α mRNA 和 IL-1β mRNA的表达明显减低。结论除湿通痹方可抑制痛风性关节炎TNF-α和IL-1β的表达而发挥抗炎作用,明显减轻关节的病理性损伤,有效抑制关节炎的临床进程。
Abstract:
Objective To investigate the influence of swelling ratio and the expression of cytokines in synovial cell of foot joint of MSU-induced rats with acute gouty arthritis of the gout one prescription, and to investigate the mechanism of the treatment of acute gouty arthritis. Methods Forty Wistar(40) male rates were randomly divided into 4 groups: the normal saline group, positive control group, group I of the gout one prescription and group Ⅱof the gout one prescription. The corresponding drugs were administered to each group, and 2 times a day, and 1.5 hours after filling the stomach in fifth day, the rats were injected into the Sodium uric acid cavity to form the model of acute gout arthritis in rats, and the swelling degree of the rats was measured at different times to observe the effect of the square on the swelling ratio of the joint; After 24 hours of injection of sodium, rats were executed and the effect of the expression of TNF-α mRNA and IL-1β mRNA in articular synovial cells was detected by RT-PCR. Results The gout one prescription can effectively inhibit the swelling of ankle joint in rats; Compared with the normal saline group, the expression of TNF-α mRNA and IL-1β mRNA was significantly decreased. Conclusion The gout one prescription can inhibit the expression of TNF-α and IL-1β in the gouty arthritis and play an anti-inflammatory effect and it can reduce the pathological damage of the joints and inhibit the clinical course of arthritis effectively.

参考文献/References

[1] Hachica M,Naccaehe PH,Mccoll SR. Inflammatory micrcrystals differentially regulate the secretion of macrophage inflammatory pratein and IL-8 by human nutrophils:a possible mechanism of neutrophic recruit-ment to sites of inflammation in synovitis[J]. J Exp Med,1995,182(6):2019-2025.
[2] 赵巴根那,董华,董清平. 纳米中药对急性痛风性关节炎IL-β、IL-6、IL-8影响的实验研究[J]. 中医药信息,2012,29(1):117-119.
[3] 万春平,李兆福,徐翔峰,等. 急性痛风性关节炎免疫学发病机制研究进展[J]. 风湿病与关节炎,2012,1(4):52-55.
[4] 黄火高,孙运峰,胡明,等. 大鼠急性痛风性关节炎模型的建立及特点[J]. 军事医学科学院院刊,2005,29(6):538-542.
[5] 黄尉霞. 药物对痛风性关节炎模型IL-1、IL-6的影响[J]. 中国中西医结合肾病杂志,2002,3(6):359-360.
[6] Coderre TJ,Wall PD. Ankle joint urate arthritis in rat:an alternatire animal model of arthritis to that produced by Freund’s adjuvant[J]. Pain,1987,28(3):379-393.
[7] 施新酋. 医用实验动物学[M]. 西安:陕西科学技术出版社,1989:418-419.
[8] 姜萍,王占奎. 清泻浊毒法对高尿酸血症大鼠尿量及尿酸水平的影响[J]. 世界中西医结合杂志,2013,8(6):605-607.
[9] 党晓芬. 王不留行抗炎、镇痛活性部位筛选及其作用机制研究[D]. 西安:陕西师范大学,2014.
[10] 陈光亮,吕红霞,王媛媛,等. 萆薢牛膝总皂苷对尿酸钠诱导的大鼠急性痛风性关节炎的防治作用[J]. 中药药理与临床,2010,26(1):34-37.
[11] 刘洪超,杨小龙,王淑英. 猪苓药理作用研究进展[J]. 河南科技大学学报(医学版),2011,29(2):159-160.
[12] 李婷,徐文珊,李西文,等. 中药桔梗的现代药理研究进展[J]. 中药药理与临床,2013,29(2):205-208.
[13] 王长林,王秀君,浦仕飞. 荆芥与防风的药理作用试验研究[J]. 郑州牧业工程高等专科学校学报,2009,29(1):6-8.
[14] 金玉青,洪远林,李建蕊,等. 川芎的化学成分及药理作用研究进展[J]. 中药与临床,2013,4(3):44-48.
[15] 王春玲. 中药地龙的活性成分与药理作用研究[J]. 亚太传统医药,2015,11(7):53-54.
[16] Choi HK,Mount DB,Reqinato AM,et al. Pathogenesis of gout[J]. Ann Intern Med,2005,143(7):499-516.

备注/Memo

备注/Memo:
* 基金项目: 云南省科技计划项目(2013FD099);云南省应用基础研究计划项目(201401SH00018) 收稿日期: 2016 - 04 - 03 作者简介: 王涛(1984-),男,云南昆明人,主治医师,研究方向:中西医结合骨伤科基础与临床。△通信作者:李雷,E-mail:183070775@qq.com
更新日期/Last Update: 2016-05-20