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亚麻子水提液对DPP-4及α-葡萄糖苷酶活性抑制实验研究(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2016年03期
页码:
14-16
栏目:
方药研究
出版日期:
2016-05-15

文章信息/Info

Title:
Experimental Study of Inhibit Activity of DPP-4 and α-glucosidasewith Flaxseed Water Extraction Liquid
作者:
张碧溦李继安田春雨周雪梅王晨斌徐雪梅焦子珊喇孝瑾△
华北理工大学中医学院,河北 唐山 063000
Author(s):
ZHANG Biwei LI Jian TIAN Chunyu ZHOU Xuemei WANG Chenbin XU Xuemei JIAO Zishan LA Xiaojin
TCM of North China University of Science and Technology,Tangshan 063000,China
关键词:
亚麻子 DPP-4 α-葡萄糖苷酶 2型糖尿病
Keywords:
flaxseed DPP-4 α-glucosidase enzyme type 2 diabetes
分类号:
R285.5
DOI:
10.19288/j.cnki.issn.1000-2723.2016.03.004
文献标识码:
A
摘要:
目的通过观察亚麻子水提液对二肽基肽酶-4(DPP-4)、α-葡萄糖苷酶抑制作用,为该药防治糖尿病提供实验依据。方法①以DPP-4酶、缓冲液、底物建立DPP-4抑制剂的体外筛选体系,对亚麻子水提液进行抑制实验,采用发色底物法测定吸光度(OD),计算DPP-4抑制率及IC50值;②以蔗糖为底物建立α-葡萄糖苷酶活性抑制模型,采用葡萄糖氧化酶法测定亚麻子水提液对α-葡萄糖苷酶的抑制作用,计算其抑制率和IC50值。结果①亚麻子具有轻度DPP-4抑制作用,其IC50值738.20mg/L;②亚麻子具有α-葡萄糖苷酶抑制作用,其IC50值为365.9mg/mL。结论亚麻子水提液可一定程度地抑制DPP-4及α-葡萄糖苷酶活性。
Abstract:
Objective Provide experimental basis through observing inhibit activity of DPP-4 and α-glucosidase with flaxseed water extraction liquid. Methods 1. Established in vitro screening system of DPP-4 inhibitor by using DPP-4 enzyme,buffer solution and substrate. Then carried on suppression experiment of flaxseed water extraction liquid and determined absorbance through chromogenic peptide substrate method. Finally,calculated the DPP-4 suppression rate and the IC50 value. 2. Took sucrose as substrate to establishα-glucosidase enzyme activity inhibition model. Then determined inhibitory effect of flaxseed water extraction liquid on α-glucosidase enzyme. Finally,calculated the inhibition rate and IC50 value. Results 1. Flaxseed can mildly inhibit DPP-4,its IC50 value was 738.20mg/L. 2. Flaxseed can inhibit α-glucosidase enzyme,its IC50 value was 365.9mg/mL. Conclusion Flaxseed water extraction liquid caninhibit the activity of DPP-4 and alpha glycosidase enzyme to a certain degree.

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备注/Memo

备注/Memo:
* 基金项目: 河北省国际合作项目(14397705D);河北省中医药管理局科研计划项目(2013066) 收稿日期: 2016 - 05 - 02 作者简介: 张碧溦(1991-),女,河北唐山人,在读硕士研究生,研究方向:中医药防治糖脂代谢疾病。△通信作者:喇孝瑾,E-mail:13730505300@163.com
更新日期/Last Update: 2016-05-20