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补阳还五汤对大鼠脊髓损伤修复与运动功能康复的作用研究(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2016年04期
页码:
1-5,15
栏目:
实验研究
出版日期:
2016-08-01

文章信息/Info

Title:
Effects of Buyang Huanwu Decoction on Motor Functional Recovery after Spinal Cord Injury in Rats
作者:
邹礼梁1王奎1满夏楠1刘学红2陈健尔1
1. 浙江中医药大学,浙江 杭州 310053;2. 绍兴文理学院,浙江 绍兴 312000
Author(s):
ZOU Liliang1 WANG Kui1 MAN Xianan1 LIU Xuehong2 CHEN Jianer1
1. Zhejiang Chinese Medical University, Hangzhou, 310053, China; 2. Shaoxing University, Shaoxing 312000, China
关键词:
脊髓损伤 补阳还五汤 剂量 胶质纤维酸性蛋白 巢蛋白
Keywords:
spinal cord injury Buyang Huanwu decoction dose glial fibrillary acidic protein Nestin
分类号:
R285.5
DOI:
10.19288/j.cnki.issn.1000-2723.2016.04.001
文献标识码:
A
摘要:
目的探讨中医补阳还五汤对大鼠脊髓损伤后脊髓组织星形胶质细胞胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)和巢蛋白(Nestin)表达的影响及其促进脊髓损伤修复的作用机制。方法大鼠随机分为假手术组(Sham 组)、模型组(Model组)、补阳还五汤低剂量组(L-BYHWD组)、补阳还五汤中剂量组(M-BYHWD组)和补阳还五汤高剂量组(H-BYHWD组)。Model组、补阳还五汤低、中、高4组大鼠行脊髓左侧半横断损伤;模型组不行药物干预,补阳还五汤低、中、高3组分别使用不同剂量补阳还五汤连续灌胃。用免疫组织化学法检测不同组大鼠经补阳还五汤治疗后不同时间段损伤区脊髓组织内GFAP和Nestin表达情况,BBB评分评估大鼠后肢运动功能。结果补阳还五汤干预后大鼠BBB评分明显高于同时期Model组大鼠(P<0.05)。脊髓损伤后大鼠脊髓组织GFAP表达出现明显上调,补阳还五汤干预后GFAP表达出现下调。M-BYHWD组和H-BYHWD组大鼠脊髓组织GFAP表达明显低于同时期Model组(P<0.05);H-BYHWD组大鼠GFAP表达在术后3~7d明显低于同时期M-BYHWD组(P<0.05),而术后14~28d二者无显著差异(P>0.05)。补阳还五汤干预的脊髓组织Nestin表达水平均高于同时期Model组(P<0.05);中、高剂量组Nestin表达水平明显高于同时期低剂量组和Model组(P<0.05),而中、高剂量组之间Nestin表达水平无显著差异(P>0.05)。结论补阳还五汤能促进大鼠脊髓损伤的修复和运动功能的康复,其作用机制可能与抑制GFAP表达减少瘢痕形成、上调Nestin表达促进神经修复有关。
Abstract:
Objective To study the effects and mechanism of Buyang Huangwu Decoction on rats with spinal cord injury by analyzing the changes of glial fibrillary acidic protein (GFAP) and Nestin expression. Methods SD rats were randomly divided into five groups:sham group, model group, low dose Buyang Huangwu Decoction group(L-BYHWD), medium dose Buyang Huangwu Decoction group(M-BYHWD) and high dose Buyang Huangwu Decoction group(H-BYHWD). Rats of Model group, L-BYHWD group, M-BYHWD group, H-BYHWD group underwent spinal cord hemisection injury;model group without drug intervention, Buyang Huanwu Decoction group were used with different doses of Buyang Huanwu Decoction orally. Immunochemistry was used to evaluate the expression level of GFAP and Nestin. BBB scales was used to evaluate the motor function of hind limbs in rats. Results The BBB scores of the rats after the intervention of BYHWD was higher than the same period of the model group(P<0.05). The expression of GFAP in the rats spinal cord tissue was significantly increased after SCI, while down-regulation in the groups with the intervention of BYHWD. The expression of GFAP in the spinal cord tissue of rats of M-BYHWD and H-BYHWD was significantly lower than the same periods in the model group(P<0.05); The expression of GFAP in the rats of H-BYHWD significantly lower than the same periods of M-BYHWD in 3-7 days after surgery(P<0.05), while it does no significant difference in 28 days after surgery(P>0.05). expression of Nestin in the spinal cord tissue with the intervention of BYHWD was higher than the same period model group(P<0.05);Both the groups of M-BYHED and H-BYHWD, the expression level of Nestin were significantly higher than the model group in the same period(P<0.05), while there was no significant difference of the Nestin expression between the M-BYHWD and the H-BYHWD. Conclusion BYHWD can promote the repair of injured spinal cord in rats and its mechanism may be related to reducing scaring by inhibiting the expression of GFAP and repairing nerve by increasing the expression of Nestin.

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备注/Memo

备注/Memo:
* 基金项目: 浙江省公益性技术应用研究计划项目(2012C33066);浙江省中医药科技计划项目(2012ZA123) 收稿日期: 2016 - 06 - 24 作者简介: 邹礼梁(1988-),男,江西赣州人,在读硕士研究生,研究方向:脊髓损伤的康复治疗。△通信作者:陈健尔,E-mail:cje28@foxmail.com
更新日期/Last Update: 2016-07-20