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重楼皂苷Ⅰ对人肝癌细胞MHCC97-H增殖、周期、凋亡的影响(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2017年03期
页码:
7-10
栏目:
实验研究
出版日期:
2017-07-17

文章信息/Info

Title:
Effects of Polyphyllin I and Sorafenib on the Proliferation and Cell Cycle and Apoptosis of MHCC97-H
作者:
曾普华1叶书林2王佳佳2李为2刘丹2贺佐梅2邓天好1周芳1郜文辉2
1. 湖南省中医药研究院,湖南 长沙 410026;2. 湖南中医药大学,湖南 长沙 410208
Author(s):
ZENG Puhua1YE Shulin2WANG Jiajia2LI Wei2LIU Dan2HE Zuomei2DENG Tianhao1ZHOU Fang1GAO Wenhui2
1. Hunan Traditional Chinese Medicine Research Institute, Changsha 410026, China;2. Hunan University of Traditional Chinese Medicine, Changsha 410208, China
关键词:
重楼皂苷 索拉菲尼 肝癌 MHCC97-H
Keywords:
polyphyllin I sorafenib cancer of the liver MHCC97-H
分类号:
R285.5
DOI:
10.19288/j.cnki.issn.1000-2723.2017.03.002
文献标识码:
A
摘要:
目的 观察重楼皂苷Ⅰ对人肝癌细胞MHCC97-H增殖、周期及凋亡的影响。方法 1. 将对数生长期的人肝癌细胞MHCC97-H,加入不同浓度的重楼皂苷Ⅰ和索拉菲尼,24h后CCK8法检测药物对细胞增殖的影响。2. 根据CCK8实验结果,选取相应浓度的重楼皂苷Ⅰ和索拉菲尼,作用于细胞24h,观察细胞周期及凋亡情况。结果 重楼皂苷Ⅰ及索拉非尼作用于人肝癌细胞MHCC97-H的IC50的浓度分别是5.7μg/mL、10μmol/mL。重楼皂苷Ⅰ及索拉菲尼均能抑制S期,将细胞阻滞于G0/G1期,能明显促进细胞凋亡,与空白对照组比较具有显著差异(P<0.01)。结论 重楼皂苷Ⅰ对人肝癌细胞MHCC97-H具有明显的抑制增殖及促进凋亡的作用。
Abstract:
Objective To observe effects of Polyphyllin I and Sorafenib on the proliferation and cell cycle and apoptosis of MHCC97-H. Methods MHCC97H cells of logarithmic growth phase was detected for cell proliferation by adding different concentrations of Polyphyllin I and sorafenib for 24 hours. 2. According to the results of CCK8, the concentration of IC50 of Polyphyllin I and Sorafenib on MHCC97-H cells was selected and interfering cells for 24 hours to observe the cell cycle and apoptosis. Results IC50 ofPolyphyllin I and sorafenib on MHCC97H cells was 5.7μg/ml、 10μmol/ml. compared with blank control group, Polyphyllin I and Sorafenib can inhibit S period, blocked the cells in G0/G1 phase, can obviously promote the cell apoptosis. There was a significant difference compared with the blank control group(P<0.01). Conclusion Polyphyllin I can significantly inhibit the proliferation of MHCC97H and promote its apoptosis.

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备注/Memo

备注/Memo:
* 基金项目: 国家自然科学基金(81603603);湖南省教育厅优秀青年项目(14B135);湖南省研究生创新基金(CX2014B364);
湖南省科技计划项目(2016SK2051);湖南省研究生创新项目(2016CX31)
收稿日期: 2017 - 05- 18
作者简介: 曾普华(1976-),男,湖南娄底人,副主任医师,研究方向:恶性肿瘤的中西结合诊治。
△通信作者:郜文辉,E-mail:476541034@qq.com
更新日期/Last Update: 2017-06-20