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蠲痹历节清方对大鼠痛风性关节炎关节肿胀指数和滑膜组织中炎症因子的影响(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2017年03期
页码:
15-18,32
栏目:
实验研究
出版日期:
2017-07-17

文章信息/Info

Title:
Effect of Juanbilijieqing Decoction on Joint Swelling Index and Inflammatory Factorsin Synovial Tissues of Rats with Gouty Arthritis
作者:
周彪13郭玉星2陆小龙2周友良1曾鹏2齐新宇1熊辉1
1. 湖南中医药大学,湖南 长沙 410208;2. 湖南中医药大学第二附属医院,湖南 长沙 410005;3. 湘潭市第一人民医院,湖南 湘潭 411101
Author(s):
ZHOU Biao13GUO Yuxing2LU Xiaolong2ZHOU Youliang1ZENG Peng2QI Xinyu1XIONG Hui1
1. Hunan University of Chinese Medicine, Changsha 410208, China;2. The Second Hospital Affiliated to Hunan University of Chinese Medicine, Changsha 410005, China;3. The First Pelple’s Hospital of Xiangtan,Xiangtan 411101,China
关键词:
蠲痹历节清方 痛风性关节炎 关节肿胀指数 炎症因子
Keywords:
Juanbilijieqing Formula gout arthritis joint swelling index inflammatory cytokines
分类号:
R285.5
DOI:
10.19288/j.cnki.issn.1000-2723.2017.03.004
文献标识码:
A
摘要:
目的 观察蠲痹历节清方对大鼠痛风性关节炎关节肿胀指数和滑膜组织中炎症因子的影响,探讨蠲痹历节清方治疗痛风性关节炎的潜在机制。方法 48只雄性SD大鼠随机分为A(正常对照)、B(模型对照)、C(蠲痹历节清方治疗)和D(阳性对照)4组,每组12只。除A组(空白对照)外,其余3组均采用局部尿酸钠盐关节腔注射的方法建立大鼠痛风性关节炎膝关节模型,A、B组予以生理盐水灌胃,C组予以蠲痹历节清方灌胃,D组予以依托考昔灌胃。观察造模前、后不同时相点测量大鼠膝关节周径,并计算出膝关节肿胀指数;采用ELISA法检测大鼠膝关节滑膜组织中的TNF-α、IL-1β、IL-6、COX-2和TGF-β的含量。结果 与A组比较,B组(模型组)大鼠的膝关节肿胀指数和滑膜组织中炎症因子(TNF-α、IL-1β、IL-6、COX-2)含量明显升高(P<0.05),而TGF-β含量明显减少(P<0.05)。与B组(模型组)比较,C组(蠲痹历节清方治疗组)大鼠膝关节肿胀指数明显较低(P<0.05),且滑膜组织中炎症因子(TNF-α、IL-1β、IL-6、COX-2)含量明显低于C组(P<0.05),而抗炎因子TGF-β含量明显高于C组(P<0.05)。结论 蠲痹历节清方能够有效缓解痛风性关节炎大鼠膝关节炎性肿胀,其作用机制可能与抑制局部组织中炎症因子TNF-α、IL-1β、IL-6、COX-2的合成、释放,同时增加局部组织中抗炎因子TGF-β的含量有关。
Abstract:
Objective To observe the effects of Juanbilijieqinfang on Tumor necrosis factor-α(TNF-α), Interleukin-6(IL-6), Interleukin-1β(IL-1β), cyclooxygenase-2(COX-2) and Transforming growth factor(TGF-β) expression levels in the knee joint synovium tissues, and explore the therapeutic mechanism of Juanbilijieqinfang in rats with monosodiumurate(MSU) crystals-induced gouty arthritis. Methods The rats were dividied into normal group, model group, Juanbilijieqinfang group, Eloricoxib group. Each group of 12. In addition to the control group, the remaining 3 groups were given on Local intra-articular injection of sodium urate gouty arthritis rat model knee, The normal group and model group were ig administered with normal saline;various treatment groups were ig administered with corresponding doses for continuous 2 days. The knee circumference was measured at different time points before and after modeling, and the knee swelling index was calculated. The levels of TNF-α, IL-1β, IL-6, COX-2 and TGF-β in synovial tissue of rat knee were detected by ELISA. Results Data from our study showed that Compared with control group, The rat with gouty arthritis induced by MSU crystal demonstrated an elevation in TNF-α, IL-6, IL-1β, COX-2 in synovium tissues(P<0.05). While the content of TGF-β was significantly decreased(P<0.05). And compared to Juanbilijieqinfang group, the rat knee swelling index in the model group was significantly lower(P<0.05), and synovial tissue of inflammatory cytokines(TNF-α, IL-1β, IL-6 and COX-2) were significantly lower than those in Juanbilijieqinfang group(P<0.05), while the levels of anti-inflammatory factor TGF-β were significantly higher than those of Juanbilijieqinfang group(P<0.05). Conclusion The mechanism of Juanbilijieqinfang can effectively eliminate the inflammatory swelling of knee joint in gouty arthritis rats. The mechanism may be related to the inhibition of TNF-α, IL-1β, IL-6 and COX-2 in local tissue, while increasing the local tissue anti-inflammatory factor TGF-β content.

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备注/Memo

备注/Memo:
* 基金项目: 国家自然科学基金(81574005)
收稿日期: 2017 - 04- 23
作者简介: 周彪(1986-),男,湖南湘潭人,博士,主治医师,研究方向:筋伤与骨病的中医药防治。
△通信作者:熊辉,E-mail:xh-hn@sina.com
更新日期/Last Update: 2017-06-20