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基于“情志致病”理论建立慢性咽炎肝郁脾虚大鼠模型的研究*(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2018年01期
页码:
11-15
栏目:
实验研究
出版日期:
2018-06-30

文章信息/Info

Title:
Study on the Type of Liver-stagnation and Spleen-deficiency Chronic Pharyngitis Rat Model that Based Emotion Pathopoiesis Theory
作者:
王艺1周家璇1黄春江1李在望2
(1. 云南中医学院第一附属医院,云南 昆明 650021;2. 南京医科大学附属无锡人民医院,江苏 无锡,214023)
Author(s):
WANG Yi1 ZHOU Jiaxuan1 HUANG Chunjiang1 LI Zaiwang2
(1. Yunnan Uinversity of TCM, Kunming 650021, China; 2. Wuxi People’s Hospital of Nanjing Medical University, Wuxi 214023 China)
关键词:
慢性咽炎 动物模型 情志致病 咽黏膜
Keywords:
chronic pharyngitis animal model emotion pathopoiesis pharyngeal mucosa
分类号:
R228
DOI:
10.19288/j.cnki.issn.1000-2723.2018.01.003
文献标识码:
A
摘要:
目的通过建立慢性咽炎肝郁脾虚大鼠模型,探讨慢性咽炎的发生发展与影响情绪的关键神经递质含量的相关性,从而研究慢性咽炎与“情志致病”的中医致病理论之间的关系。方法选用70 只Wistar大鼠构建慢性咽炎模型,随机分7 组:空白对照组,标准慢性咽炎模型组,标准慢性咽炎模型低剂量组,标准慢性咽炎模型高剂量组,慢性咽炎肝郁脾虚模型组,慢性咽炎肝郁脾虚低剂量组,慢性咽炎肝郁脾虚高剂量组,造模完成并评估后分别给予“咽炎消合剂”治疗10 d,检测各组大鼠下丘脑组织5HT-mRNA表达,并对各组大鼠行咽黏膜病理切片检测。结果2种模型大鼠下丘脑组织5HT-mRNA表达低于空白组,肝郁脾虚组的5-HT2C含量明显低于标准模型组,5-HT1B高于标准模型组。经予“咽炎消合剂”治疗后,5-HT1B、5-HT2C受体增加。病理切片显示与2种模型对比治疗前,其咽黏膜慢性炎细胞的数量减少,咽部血管通透性及炎细胞渗出减轻。结论慢性咽炎肝郁脾虚大鼠模型建立成功,比标准模型更合乎临床病理特征,证实大鼠体内慢性咽炎的发生发展与影响情绪的关键物质神经递质的异常表达有直接的关联,证明了慢性咽炎中医致病理论“情志致病”的正确性。
Abstract:
Objective To confirm the theory of emotion pathopoiesis that basis of TCM, through establishing a rat model of chronic pharyngitis(CP) liver-stagnation and spleen-deficiency CP rat model(GYPXM) to explore the correlation between the occurrence and development of CP and the expression of the key neurotransmitter. Methods 70 wistar rats were used to establishing the CP standard model and liver-stagnation and spleen-deficiency CP rat model, that divided into 7 groups randomly. After modeling, the hypothalamus tissue 5HT mRNA expression in each group was examined, and pathological sections of rats in each group were observed. Results The hypothalamus 5HT mRNA expression in both models was lower than that in the blank group. After 10 days of Yanyanxiao mixture treatment, the 5-HT1B, 5-HT2C receptor two kind of rat model were increased. Conclusion Compared with the standard model , CP rat model of liver-spleen deficiency established successfully, and is more suitable for clinical and pathological features. The incidence of CP is associated with abnormal expression of the key neurotransmitter which affectded by mood.

参考文献/References

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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金(81760881);云南省科技厅应用基础研究计划项目(2014FZ094) 收稿日期: 2018 - 01- 15 作者简介: 王艺(1980-),男,博士,主治医师,研究方向:咽喉疾病的中西结合临床及理论研究。 通信作者: 李在望,E-mail:lizaiwang123@hotmail. com
更新日期/Last Update: 2018-06-30