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薏苡仁提取物调节大鼠非酒精性脂肪肝病游离脂肪酸的代谢作用机制(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2018年01期
页码:
16-19
栏目:
实验研究
出版日期:
2018-06-30

文章信息/Info

Title:
Effect of Coix Extract on the Metabolism of NAFLD Free Fatty Acid in Rats
作者:
朱凯陈壮黄金龙王凯华
(广西中医药大学附属瑞康医院,广西 南宁 530011)
Author(s):
ZHU Kai CHEN Zhuang HUANG Jinlong WANG Kaihua
(Ruikang Hospital Affiliated to Guangxi University of Traditional Chinese Medicine, Nanning 530011, China)
关键词:
薏苡仁提取物 大鼠 非酒精性脂肪肝病 游离脂肪酸 代谢
Keywords:
Coix extract rats nonalcoholic fatty liver disease(NAFLD) free fatty acid metabolism
分类号:
R285.5
DOI:
10.19288/j.cnki.issn.1000-2723.2018.01.004
文献标识码:
A
摘要:
目的探讨薏苡仁提取物调节大鼠非酒精性脂肪肝病(NAFLD)游离脂肪酸的代谢作用机制。方法选取成年大鼠30只制作NAFLD模型,随机分为模型组、高剂量组、低剂量组各10只,同时选取健康大鼠10只为对照组,对比4组大鼠代谢指标。结果与对照组比较,模型组的TC、FFA上升,同时ADP下降(P<0.05),治疗组TC、FFA明显低于模型组,同时ADP高于模型组(P<0.05),不同剂量大鼠组间对比差异有统计学意义(P<0.05);同正常组比较,模型组的ALT以及AST明显上升(P<0.05),治疗组ALT、AST水平明显低于模型组(P<0.05),高剂量组低于低剂量组大鼠(P<0.05);模型组AMPK活性降低,同时FAS、ACCa-se等表达明显上升(P<0.05),治疗组AMPK活性上升,同时FAS、ACCa-se等表达明显下降(P<0.05),不同剂量大鼠组间对比差异有统计学意义(P<0.05)。结论薏苡仁提取物能有效提高NAFLD大鼠脂联素水平,诱发下游的脂质代谢反应,从而改善游离脂肪酸的代谢。
Abstract:
Objective To investigate the metabolic mechanism of the extract of Coix seed to regulate free fatty acid(fats) of NAFLD(non-alcoholic fatty liver disease) in rats. Methods 30 adult rats were selected to make NAFLD models. They were randomly divided into model group, high-dose group and low dose group with 10 rats in each group. Meanwhile, 10 healthy rats were selected as control group, and the metabolic indexes of four groups were compared. Results Compared with control group rats, the rats in the model group TC, FFA increased and ADP decreased(P<0.05), the rats in the treatment group TC, FFA and ADP was significantly lower than that of model group was higher than in the model group(P<0.05), different doses of rats to form comparison have significant difference(P<0.05); compared with normal rats, the rats in the model group significantly increased ALT and AST(P<0.05), the treatment of ALT and AST levels in rats was significantly lower than that of model group(P<0.05), the high dose group rats were significantly lower than that of low dose group rats(P<0.05); AMPK activity model rats were lower and FAS, ACCa-se expression increased significantly(P<0.05), the treatment group rats increased AMPK activity and FAS, ACCa-se expression decreased significantly(P<0.05), different doses of rats to form comparison have significant difference(P<0.05). Conclusion The extract of Coix seed can effectively improve the level of adiponectin in NAFLD rats and induce the lipid metabolic reaction in the lower reaches, thus improving the metabolism of free fatty acids.

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备注/Memo

备注/Memo:
作者简介: 朱凯(1977-),男,硕士,主治医师,从事中医内科工作。
更新日期/Last Update: 2018-06-30