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基于NF-κB通路动态观察通督调神针刺对动脉硬化性脑梗死模型大鼠Chemerin水平的影响*(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2018年01期
页码:
20-24
栏目:
实验研究
出版日期:
2018-06-30

文章信息/Info

Title:
Effect of Tongdu Tiaoshen Acupuncture on Chemerin Level in Rats with Arteriosclerotic Cerebral Infarction Based on Dynamic Observation of NF-κB Pathway
作者:
吴海洋王颖韩为王海涛
(安徽中医药大学第二附属医院,安徽 合肥 230000)
Author(s):
WU Haiyang WANG Ying HAN Wei WANG Haitao
(The Second Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei 230000, China)
关键词:
通督调神 针刺 NF-κB Chemerin 动脉硬化性脑梗死
Keywords:
Tongdu Tiaoshen acupuncture NF-κB Chemerin arteriosclerotic cerebral infarction
分类号:
R245
DOI:
10.19288/j.cnki.issn.1000-2723.2018.01.005
文献标识码:
A
摘要:
目的基于NF-κB通路观察通督调神针刺方法对动脉硬化性脑梗死模型大鼠Chemerin水平的动态变化,寻找针刺最佳干预时间点。方法选取96 只Wistar雄性大鼠随机分为对照组(基础饲料喂养)、假手术组(基础饲料喂养+颈动脉分离)、模型组(高脂饲料喂养+线栓法造脑梗死模型)和针刺组(高脂饲料喂养+线栓法造脑梗死模型+针刺)4组。根据缺血再灌注6、24、48 h各分为3 个亚组,每组8 只。其中模型组和针刺组采用Zea Long线栓法造模成功后,根据Zea Longa的神经功能评分,将评分1~3 分大鼠收入相应组别。4 组采用流式细胞仪测定血清中NF-κB的表达活性;运用酶联免疫吸附试验(ELISA)法测定血清中Chemerin水平。结果①Zea Longa的神经功能评分,针刺6 h组神经功能缺损评分较同组其他时间点降低更为显著(P<0.01);②假手术组血清中Chemerin和NF-κB的水平与对照组比较差异无统计学意义(P>0.05);③模型组血清中Chemerin和NF-κB的水平与对照组和假手术组比较显著上调(P<0.01)。④缺血再灌注6 h NF-κB水平开始升高,缺血再灌注24 h和48 h NF-κB水平仍在持续升高,缺血再灌注6 h针刺组血清中NF-κB水平与同组不同时间点水平相比下调最为显著(P<0.01);⑤缺血再灌注6 h针刺组血清中Chemerin水平与同时间点模型组相比差异具有统计学意义(P<0.01)。缺血再灌注24 h和48 h针刺组血清中Chemerin水平与同时间点模型组相比差异无统计学意义(P>0.05)。结论①通督调神针刺对于动脉硬化性脑梗死模型大鼠神经功能缺损具有显著修复作用。②通督调神针刺可以抑制动脉硬化性脑梗死模型大鼠血清中NF-κB活性,降低Chemerin水平。在缺血再灌注6 h为最佳针刺时间点。
Abstract:
Objective To observe the dynamic changes of Chemerin levels in rats with arteriosclerotic cerebral infarction treated by Tongdu Tiaoshen acupuncture method based on the NF-κB pathway and to find the optimal time for acupuncture intervention. Methods Ninety-six adult Wistar male rats were randomly divided into four groups:control group, sham operation group, model group and acupuncture group. According to ischemia-reperfusion 6 h, 24 h, 48 h each is divided into 3 subgroups, 8 in each group. According to the neurological function score of Zea Longa, the standard rat income will be matched to the corresponding group, and the expression activity of NF-KB in peripheral blood mononuclear cells will be determined by flow cytometry. After enzyme-linked immunosorbent assay(ELISA)Determination of Chemerin levels in peripheral blood. Results ①Neurological score of Zea Longa;②Chemerin and NF-κB levels in sham group were not significantly different from those in blank group(P>0.05);③Chemerin and The level of NF-κB was significantly up-regulated compared with the blank group and the sham operation group(P<0.01). ④The level of NF-κB began to increase at 6 h after ischemia-reperfusion, and the levels of NF-κB in 24 h and 48 h after ischemia-reperfusion continued to increase. But the level of NF-κB in acupuncture group at 6 h after ischemia-reperfusion was the most significant(P<0.01). ⑤Chemerin levels in the acupuncture group at 6 h after ischemia-reperfusion were significantly different from those in the model group at the same time point(P<0.01). The levels of Chemerin in the acupuncture group at 24 h and 48 h after ischemia-reperfusion were not significantly different from those in the model group at the same time point(P>0.05). Conclusion ①Tongdu Tiaoshen acupuncture can significantly reduce atherosclerosis cerebral infarction Neurological deficit in model rats. ②Tongdu Tiaoshen acupuncture can inhibit NF-κB activity and reduce Chemerin in peripheral blood of rats with atherosclerotic cerebral infarction. Acupuncture at 6 h after ischemia-reperfusion was the best treatment time point.

参考文献/References

[1] SPIROGLOU S G,KOSTOPOULOS C G,VARAKIS J N,et al. Adipokines in periaortic and epicardial adipose tissue:differential expression and relation to atherosclerosis[J]. J Atheroscler Thromb,2010,17(2):115-130
[2] MCCARTHY T C,ZUNIGA L A,ZABEL B A,et al. The novel adipokine chemerin significantly increases cholesterol uptake in human macrophages[J]. FASEB J,2008,22(4):948-952.
[3] 仲英洁,张子为,徐郁,等. 阿托伐他汀对ApoE-/-小鼠主动脉粥样硬化和钙化的影响[J]. 中国动脉硬化杂志,2013,21(4):305-310.
[4] 宋海彬,张羽,关坤萍,等. 瑞舒伐他汀对人单核-巨噬细胞组织因子表达的影响[J]. 中国医师杂志,2012,14(2):173-176.
[5] 杨俊,岳增辉,谢涛,等. 动脉粥样硬化易损斑块与TLR4/NF-κB信号通路关系的研究进展[J]. 中西医结合心脑血管病杂志,2014,12(6):747-749
[6] 谢霆,陈新忠,董念国,等. NF-κB途径介导Chemerin抑制THP-1源性巨噬泡沫细胞ABCA1表达和降低胆固醇外流[J]. 华中科技大学学报(医学版),2012,41(4):389-394.
[7] LONGA E Z,WEINSTEIN P R,CARLSON S,et al. Reversible middle cerebral artery occlusion without craniectomy in rats[J]. Stroke,1989(1):84-91.
[8] 郭义. 实验针灸学[M]. 北京:中国中医药出版社,2008:414-417.
[9] 李伟君,李应东. 心肌缺血再灌注损伤钙超载及中医药防治策略[J]. 中西医结合心脑血管病杂志,2013,11(2):217-218.
[10] 陈蓓蕾,李晓波,李军,等. 短暂性脑缺血发作与IL-6、IL-8和IL-10的关系[J]. 脑与神经疾病杂志,2013,21(4):247-250.
[11] BROWN G C. Nitric oxide and neuronal death[J]. Nitric Oxide,2010,23(3):153-165.
[12] 吴海洋. 通督调神针刺对脑缺血再灌注模型大鼠TNF-α和CRP水平的动态观察[D]. 合肥:安徽中医药大学,2014.
[13] ZHANG S,QI Y,XU Y,et al. Protective effect of flavonoid-rich extract from Rosa laevigata Michx on cerebral ischemia-reperfusion injury through suppression of apoptosis and inflammation[J]. Neurochem Int,2013,63(5):522-532.
[14] ZHANG X,ZHANG X,WANG C,et al. Neuroprotection of early and short-time applying berberine in the acute phase of cerebral ischemia:up-regulated pAkt,pGSK and pCREB,down-regulated NF-κB expression,ameliorated BBB permeability[J]. Brain Research,2012,1459:61-70.
[15] 于凌志,左艳丽,贾孟辉,等. 核转录因子NF-κB对脑缺血再灌注干预机制的研究进展[J]. 中国民族医药杂志,2015(7):42-44.
[16] 万东,罗勇,谢鹏. 电刺激小脑顶核对缺血/再灌注大鼠脑组织内NF-κB活性及其活化的影响[J]. 中华物理医学与康复杂志,2006,28(10):660-665.
[17] LUANGSAY S,WITTAMER V,BONDUE B,et a1. Mouse ChemR23 Is expressed in dendritic cell subsets and macrophages,and mediates an anti-inflammatory activity of chemerin in a lung disease model[J]. J Immunol,2009,183(10):6489-6499.
[18] ROMAN A A,PARLEE S D,SINAL C J. Chemerin:a potential endocrine link between obesity and type 2 diabetes[J]. Endocrine,2012,42(2):243-251.
[19] RHEE E J. Chemerin:a nove link between inflammation and atherosclerosis[J]. Diabetes Metab J,2011,35(3):216-218.
[20] 张玲,张国庆,朱玲玲,等. 通督调神针刺法治疗风痰瘀阻型急性脑梗死临床观察[J]. 安徽中医药大学学报,2017,36(4):59-62.
[21] 郑仕平,韩为,储浩然,等. 通督调神针灸预处理对脑缺血再灌注大鼠miRNA664 及MMP9 调控机制的研究[J]. 上海针灸杂志,2016,35(1):76-80.
[22] 孙培养,储浩然,李佩芳,等. 通督调神针法对脑卒中后抑郁模型大鼠血浆单胺类神经递质的影响[J]. 针灸临床杂志,2013,29(11):33-36.
[23] 孙培养,储浩然,李佩芳,等. 通督调神针刺干预对药物治疗脑卒中后抑郁的影响[J]. 中国针灸,2015,35(5):753-757.

备注/Memo

备注/Memo:
基金项目: 安徽中医药大学科研项目(2016qn022) 收稿日期: 2017 - 12- 16 作者简介: 吴海洋(1986-),男,主治医师,研究方向:针刺临床应用及机理研究。 通信作者: 王颖,Email:zhenjiu205@126.com
更新日期/Last Update: 2018-06-30