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|本期目录/Table of Contents|

外膝眼透刺内膝眼温针灸对膝骨性关节炎兔软骨组织Bax、Bcl-2表达的影响*(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2018年01期
页码:
29-33
栏目:
实验研究
出版日期:
2018-06-30

文章信息/Info

Title:
Effects of Bax and Bcl-2 in Knee Cartilage of Knee Osteoarthritis of Rabbits by Warm-Needle-Moxibustion at Neixiyan(EX-LE4) and Waixiyan(EX-LE5)
作者:
王舰12王巧灵3林淑芳12
(1. 福建中医药大学附属康复医院,福建 福州 350003;2. 福建省康复产业研究院,福建 福州 350003; 3. 福建医科大学附属泉州第一医院,福建 泉州 362000)
Author(s):
WANG Jian12 WANG Qiaoling3 LIN Shufang12
(1. Rehabilitation Hospital affiliated To Fujian University of Traditional Chinese Medicine, Fuzhou 350003, China 2. Fujian provincial rehabilitation industrial institution, Fuzhou 350003, China 3. Quanzhou First Hospital Of Affiliated to Fujian Medical University, Quanzhou 362000, China)
关键词:
膝骨性关节炎 温针灸 Bax Bcl-2
Keywords:
knee osteoarthritis warm-needle-moxibustion Bax Bcl-2
分类号:
R245
DOI:
10.19288/j.cnki.issn.1000-2723.2018.01.007
文献标识码:
A
摘要:
目的观察外膝眼透刺内膝眼温针灸对膝骨性关节炎兔软骨组织Bax mRNA、Bcl-2 mRNA及Bax、Bcl-2蛋白表达的影响。方法按随机数字表法将18只新西兰兔随机分为正常对照组(正常组)、膝骨性关节炎组(模型组)和温针内、外膝眼穴组(温针组)。4%木瓜蛋白酶关节腔注射法制作膝骨性关节炎模型。温针组在造模后行内外膝眼穴温针灸治疗20 min,隔天1次,每2 周为1个疗程,中间休息2 d,共治疗4个疗程。模型组和正常组不予任何治疗。RT-qPCR法和Western Blotting法检测软骨组织Bax mRNA、Bcl-2 mRNA和Bax、Bcl-2蛋白表达。结果①温针灸干预后,新西兰兔的精神、食欲、膝关节活动能力等均明显改善。②模型组与正常组比较,膝关节软骨组织Bax mRNA和蛋白表达均显著升高(P<0.001,P<0.01),Bcl-2 mRNA和蛋白表达均明显降低(P<0.001,P<0.001);温针组与模型组比较,温针组Bax mRNA表达显著降低(P<0.05),Bcl-2 mRNA和蛋白表达均明显升高(P<0.05,P<0.05)。结论温针内、外膝眼穴能下调软骨细胞促凋亡基因Bax 、上调抗凋亡基因Bcl-2 的表达,抑制软骨细胞凋亡通路的信号转导。
Abstract:
Objective To observe the regulatory effects Bax, Bcl-2 mRNA and protein in knee cartilage of rabbits by knee osteoarthritis of warm-needle-moxibustion at neixiyan(EX-LE4) and waixiyan(EX-LE5). Methods 18 New Zealand rabbits were randomly divided into normal control group(normal group), knee osteoarthritis group(model group) and warm-needle-moxibustion at neixiyan(EX-LE4) and waixiyan(EX-LE5) group(WNM group) using the randomized controlled Methods. The knee osteoarthritis model was made by injecting four percent of papain into the joint cavity. The WNM group were received warm-needle-moxibustion at neixiyan(EX-LE4) and waixiyan(EX-LE5) for 20 min, one treatment for two days, every 2 weeks is a course of treatment, there are rest 2 days between 2 course, after 4 courses of treatment. The model group and the normal group were not given any treatment. The expressions of Bax mRNA、Bcl-2 mRNA and protein in knee cartilage tissue was detected by RT-qPCR and Western-Blotting. Results ① Compared with the model group, the spirit, appetite and knee joint activities of rabbits in the WNM Group were obviously improved. ② The expressions of Bax mRNA and protein in the model group were both higher than those in normal group(P<0.001, P<0.01), and the expressions of Bcl-2 mRNA and protein in the model group were both lower than those in normal group(P<0.001, P<0.001). Compared with the model group, the expressions of Bax mRNA in the WNB group were significantly decreased(P<0.05), and the expressions of Bcl-2 mRNA and protein in the model group were both obviously improved(P<0.05, P<0.05). Conclusion Warm-needle-moxibustion at neixiyan(EX-LE4)and waixiyan(EX-LE5)can down-regulate the expression of Bax mRNA in knee cartilage of rabbits with knee osteoarthritis, and can up-regulate the expression of Bcl-2 mRNA and protein to inhibit the signal transduction of chondrocyte apoptosis pathway.

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备注/Memo

备注/Memo:
基金项目: 福建省自然科学基金项目(2016J01379);福建省科技厅科技平台建设项目(2015Y2001) 收稿日期: 2017 - 12- 28 作者简介: 王舰(1971-),男,副主任医师,研究方向:神经及骨科康复。
更新日期/Last Update: 2018-06-30