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冬连三七组分调控AGEs/p38MAPK改善糖尿病动脉粥样硬化兔氧化应激的机理研究(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2018年02期
页码:
1-5
栏目:
实验研究
出版日期:
2018-11-10

文章信息/Info

Title:
Effects of Composition of Ophiopogon Polysaccharide, Notoginseng total saponins and Rhizoma Coptidis Alkaloids on the Inflammatory Response of Atherosclerosis Rabbit Modle by Controlling p38MAPK
作者:
靳昭辉1靳冰1△高普1宋光熠2刘征堂1相田园1
1. 中国中医科学院西苑医院,北京 100091;2. 辽宁省基础医学研究所,辽宁 沈阳 110005
Author(s):
JIN Zhaohui1 JIN Bing1 GAO Pu1 SONG Guangyi2 LIU Zhengtang1 XIANG Tianyuan1
1. Geriatric Research Center of Xiyuan Hospital of China Academy of Traditional Chinese Medicine, Beijing 100091, China; 2. Liaoning Basic Medical Research Institute, Shenyang 110005, China
关键词:
冬连三七组分 糖尿病动脉粥样硬化 AGEs/p38MAPK信号通路 氧化应激反应
Keywords:
composition of Ophiopogon polysaccharide Notoginseng total saponins and Rhizoma Coptidis alkaloids traditional Chinese medicine composition diabetic atherosclerosis p38MAPK signaling pathway Oxidative stress reaction
分类号:
R285.5
DOI:
10.19288/j.cnki.issn.1000-2723.2018.02.001
文献标识码:
A
摘要:
目的研究冬连三七组分(Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids CONR)调控AGEs/p38MAPK信号转导通路改善糖尿病动脉粥样硬化(diabetic atherosclerosis,DA)兔氧化应激反应。方法设立空白组、模型组、辛伐他汀组、CONR高剂量组、CONR低剂量组、SB203580组;脉推注四氧嘧啶并且配合腹主动脉内膜球囊损伤术诱导雄性纯种新西兰大白兔的DA模型,实验组分别每日给予CONR450 mg/kg、CONR 150 mg/kg;辛伐他汀3 mg/kg灌胃10周,12 h重复腹腔注射SB203580 2 mg/kg 10周;正常组每日给予生理盐水20mL灌胃作为对照。结果实验各组对AGEs均有降低作用,对p-p38MAPK蛋白高表达均有抑制作用,与模型组比较差异有统计学意义。CONR高、低剂量组、辛伐他汀组对p-p38MAPK蛋白的抑制作用优于SB203580组(P<0.01),CONR高、CONR低剂量组、辛伐他汀组之间差异无统计学意义(P>0.05)。模型组大兔血清MDA高于空白组,有统计学差异(P<0.01)。给药后,CONR高剂量组、SB203580组及辛伐他汀组显著降低MDA(P<0.01)。模型组SOD活性显著降低(P<0.01),实验各组显著增强SOD活性(P<0.01)。结论冬连三七组分可能通过干预AGEs/p38MAPK信号转导通路改善DA兔的氧化应激反应。
Abstract:
Objective The purpose of this study was to explore the effects of composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids(CONR) on Oxidative stress reaction of diabetic atherosclerotic rabbit modle by interventing AGEs/p38 mitogen-acti-vatedproteinkinase(p38MAPK) signal pathway. Methods Setting up blank group, model group, CONR high-dose group, CONR low-dose group, SB203580 group, simvastatin group. DA rabbits were established by alloxan intravenous injection with abdominal aorta intima balloon injury, the experimental groups were daily given CONR 450 mg/kg and CONR 150 mg/kg, Simvastatin 3mg/kg for 10 weeks, SB203580(p38MAPK inhibitor) 2 mg/kg intraperitoneal injection, 12 h repeated for 10 weeks; in the control group, 20 mL of saline was daily administered. Rusults The serum AGEs of the model group were higher than that in the blank group, and the difference was statistically significant(P<0.01). After 10weeks of administration, the experimental groups had reduced effect on AGEs, which was statistically significant compared with the model group. The high expression of p-p38mapk protein in each group were inhibited significantly compared with the model group(P<0.01). The inhibitory effect of p-p38MAPK protein in CONR high and low dose group, simvastatin group were better than that in SB203580 group(P<0.01). There was no statistically significant difference between CONR high and low dose group and simvastatin group(P<0.01), there was statistically significant difference compared with normal control group(P<0.01). The MDA in model group were higher than that in blank group. After the treatment, CONR high dose group, the SB203580 group and simvastatin group has lower effect on MDA in DA big rabbit(P<0.01); The SOD activity was significantly enhanced in the experimental groups(P<0.01). Conclution The traditional Chinese medicine composition may be able to inhibit Oxidative stress reaction of atherosclerotic rabbit modle by interventing p38MAPK signal pathway.

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备注/Memo

备注/Memo:
收稿日期: 2018 - 03- 09 * 基金项目: 国家自然科学基金(81374026);中国中医科学院名医名家传承项目(05-78) 第一作者简介: 靳昭辉(1984-),女,中国中医科学院西苑医院博士后,主治医师,研究方向:老年病的中医药基础与临床 研究。通信作者: 靳冰,E-mail:lordnec@126.com
更新日期/Last Update: 2018-04-28