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当归补血汤对骨髓干细胞移植急性心肌梗死模型大鼠 NF-κB介导的炎症反应影响*(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2018年04期
页码:
21-24
栏目:
实验研究
出版日期:
2019-03-25

文章信息/Info

Title:
Effect of Danggui Buxue Decoction on NF-κB Mediated Inflammatory Reaction in Acute Myocardial Infarction Model of Bone Marrow Stem Cell Transplantation
文章编号:
1000 - 2723(2018)04 - 0021 - 04
作者:
彭丹江倩张丹覃松柏△
(荆州市中医医院心血管病科,湖北 荆州 434000)
Author(s):
PENG Dan JIANG Qian ZHANG Dan QIN Songbai
(Department of Cardiovascular Diseases, Jingzhou Hospital of Traditional Chinese Medicine, Jingzhou 434000, China)
关键词:
急性心肌梗死 骨髓干细胞移植 当归补血汤 NF-κB 炎症反应
Keywords:
acute myocardial infarction bone marrow stem cell transplantation Danggui Buxue Decoction NF-κB inflammation
分类号:
R285.5
DOI:
10.19288/j.cnki.issn.1000-2723.2018.04.005
文献标识码:
A
摘要:
目的探讨当归补血汤对骨髓干细胞移植急性心肌梗死模型大鼠NF-kB介导的炎症反应影响。方法选择72例健康雄性SD大鼠为研究对象,均经实验成功建立AMI模型,随机选择24例为对照组,另选择24例为观察组,剩余24例为模型组。模型组应用常规生理盐水灌胃,对照组应用骨髓干细胞移植治疗,观察组则在对照组基础上应用当归补血汤灌胃。2 w后观察各组大鼠心功能、心肌NF-kB蛋白表达和心肌凋亡情况,以及NF-kB介导的炎症反应情况。结果观察组和对照组LVIDs、LVIDd水平均分别低于模型组,LVEF水平分别高于模型组,差异有统计学意义(P<0.05);观察组LVIDs、LVIDd水平均低于对照组,LVEF水平高于对照组,差异有统计学意义(P<0.05)。观察组和对照组NF-kB表达水平及心肌凋亡指数均分别低于模型组,差异有统计学意义(P<0.05);观察组NF-kB表达水平及心肌凋亡指数低于对照组,差异有统计学意义(P<0.05)。观察组和对照组IL-6、TNF-α水平均分别低于模型组,差异有统计学意义(P<0.05);观察组IL-6、TNF-α水平低于对照组,差异有统计学意义(P<0.05)。结论在骨髓干细胞移植急性心肌梗死模型大鼠中应用当归补血汤效果明显,可通过减轻NF-kB介导的炎症反应,有效减少心肌细胞凋亡,改善大鼠心肌损伤,促使心肌功能恢复。
Abstract:
Objective To investigate the effect of Danggui Buxue Decoction on NF-kB mediated inflammatory reaction in acute myocardial infarction model in rats with bone marrow stem cell transplantation. Methods Seventy-two healthy male Sprague-Dawley(SD) rats were selected for the study. Successful AMI models were established. All the subjects randomly selected 24 cases as the control group, the other 24 cases as the observation group, and the remaining 24 cases as the model group. The control group was treated with conventional saline gavage. The control group was treated with bone marrow stem cell transplantation. The observation group was treated with Danggui Buxue Decoction by gavage on the basis of the control group. After 2 weeks, the cardiac function, myocardial NF-kB protein expression, myocardial apoptosis, and NF-kB mediated inflammatory reaction were observed in each group. Results The LVIDs and LVIDd levels in the observation group and the control group were lower than those in the model group respectively, and the LVEF levels were higher than those in the model group. The difference was statistically significant(P<0.05). The LVIDs and LVIDd levels were lower in the observation group than in the control group, and the LVEF levels were higher in the observation group than in the control group(P<0.05). The expression of NF-kB and myocardial apoptosis index were lower in the observation group and the control group than in the model group, and the difference was statistically significant(P<0.05). The expression level of NF-kB and myocardial apoptosis index were lower in the observation group than in the control group, and the difference was statistically significant(P<0.05). The levels of IL-6 and TNF-α in the observation and control groups were lower than those in the model group, and the difference was statistically significant(P<0.05). The levels of IL-6 and TNF-α in the observation group were lower than those in the control group, and the difference was statistically significant(P<0.05). Conclusion Danggui Buxue Decoction is effective in the treatment of bone marrow stem cell transplantation in rats with acute myocardial infarction. It can reduce NF-kB mediated inflammatory response, effectively reduce myocardial cell apoptosis, improve myocardial injury in rats, and promote myocardial function recovery.

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备注/Memo

备注/Memo:
收稿日期: 2018 - 07- 08
*基金项目: 湖北卫生人才科研专项计划项目(2015KKE78)
第一作者简介: 彭丹(1981-),女,主治医师,研究方向:心血管病的中西医结合治疗。
△通信作者: 覃松柏,E-mail:565248558@qq.com
更新日期/Last Update: 2018-10-30