我们的网站为什么显示成这样?

可能因为您的浏览器不支持样式,您可以更新您的浏览器到最新版本,以获取对此功能的支持,访问下面的网站,获取关于浏览器的信息:

|本期目录/Table of Contents|

三七止血和活血活性部位的筛选(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2018年06期
页码:
12-18
栏目:
实验研究
出版日期:
2019-06-30

文章信息/Info

Title:
Screening of Active Fractions of Hemostatic Effect and Activating Blood Circulation Effectof Panax notoginseng(Burk.)F. H. Chen.
文章编号:
1000 - 2723(2018)06- 0012 - 07
作者:
黄加文123李 倩123杨和金123袁名睿123肖茹月123李晋玉123苏 钛123王自梁123△
(1. 云南省药物研究所,云南 昆明 650111;2. 云南白药集团创新研发中心,云南 昆明 650111;3. 云南省中药和民族药新药创制企业重点实验室,云南 昆明 650111)
Author(s):
HUANG Jiawen123 LI Qian123 YANG Hejin123 YUAN Minrui123XIAO Ruyue123 LI Jinyu123 SU Tai123 WANG Ziliang123
(1. Yunnan Institute of Materia Medica, Kunming 650111, China;2. Yunnan Baiyao Group Innovation and R&D Center, Kunming, 650111, China;3. Yunnan Province Company Key Laboratory for TCM and Ethnic Drug of New Drug Creation, Kunming, 650111, China)
关键词:
三七 大孔树脂 分析检测 活性部位
Keywords:
Panax notoginseng(Burk.) F. H. Chen macroporous resin determination active fraction
分类号:
R284.1;R285.5
DOI:
10.19288/j.cnki.issn.1000-2723.2018.06.003
文献标识码:
A
摘要:
目的对三七不同洗脱部位成分进行分析,并筛选止血与活血活性部位。方法采用70%乙醇回流提取,大孔树脂柱划段制备样品;紫外分光光度法测定总皂苷含量,HPLC法测定三七素和5种主要皂苷的含量;小鼠断尾止血和玻片凝血实验观察样品的止血作用;肾上腺素—胶原蛋白诱导小鼠体内血栓形成实验观察样品的抗血栓作用;高分子右旋糖酐致大鼠急性血瘀实验观察样品的活血散瘀作用。结果制备得到总提物、水洗脱部位、30%乙醇洗脱部位、70%乙醇洗脱部位的三七总皂苷的含量分别为:32.51%、0%、52.39%、83.61%;三七素集中在水洗脱部位(2.78%);总提物、三个洗脱部位均能明显缩短出血时间与凝血时间,但水洗脱部位缩短凝血时间的作用最显著;总提物和30%乙醇洗脱部位能明显提高小鼠偏瘫恢复率;总提物、30%和70%乙醇洗脱部位能明显降低大鼠血液在不同切变率下的黏度。结论在本实验设计与条件下,三七素仅存在于水洗脱部位中,三七皂苷在30%和70%乙醇洗脱部位中均有分布,但种类与含量存在较大差异;水洗脱部位为三七止血主要活性部位;30%乙醇洗脱部位为三七抗血栓主要活性部位;30%和70%乙醇洗脱部位为三七活血散瘀主要活性部位。
Abstract:
Objective To analyse on the components of eluted fractions of Panax notoginseng(Burk.) F. H. Chen. Screening the active fractions by observing the hemostatic effect, antithrombotic effect, activating blood circulation and resolving stasis effect. Methods The medicinal materials were extracted by 70% ethanol, and fractions prepared by macroporous resin. The content of total saponins was determined by UV spectrophotometry, the content of dencichine and five major saponins were determined by HPLC. Bleeding time was measured by tail-cutting method, coagulation time was measured by the slide method. Collagen-adrenalin was used to induce thrombus in mice. Rats with acute blood stasis model were established by intravenous injection of high molecular dextran. Results The samples were prepared, included: the extract, water eluted fraction, 30% and 70% ethanol eluted fraction. The content of notoginseng total saponins were 32.51%, 0%, 52.39%, 83.61%. The dencichine exists in water eluted fraction only(2.78%). The extract and fractions could shorten the bleeding and coagulation time significantly. However, the water eluted could shorten coagulation time more significantly. The extract and 30% ethanol elution fraction could improve the recovery rates of hemiplegic mice significantly. Extract, 30% and 70% water eluted fractions could decrease the blood viscosities at all shear rates, significantly. Conclusion The dencichine only exists in water eluted fraction, saponins exist in 30% and 70% ethanol eluted fractions, but their contents and types were different. Water eluted fraction is main active fraction on hemostasis. The 30% ethanol eluted fraction show the obvious antithrombotic effect, and it’s main active fraction. The 30% and 70% ethanol eluted fractions show the obvious activating blood circulation and resolving stasis effect, and they are the main active fraction.

参考文献/References


[1] 黄天卫. 浅谈文山三七产业发展的现状及对策[J]. 云南科技管理,2003,16(5):60-63.
[2] 国家药典委员会. 中国药典. 一部[S]. 北京:中国医药科技出版社,2015:12.
[3] 冯陆冰,潘西芬,孙泽玲. 三七的药理作用研究进展[J]. 中国药师,2008(10):1185-1187.
[4] 李琳,王承潇,曲媛,等. 反相离子对色谱法测定三七中三七素含量[J]. 中国中药杂志,2015,40(20):4026-4030.
[5] 赵昶灵,杨生超,陈中坚,等. 紫、绿三七的总花色苷和总皂苷含量及皂苷单体组成[J]. 中药材,2014,37(10):1749-1753.
[6] 万晓青,夏伯侯,楼招欢,等. 三七不同炮制品中皂苷类成分的含量比较[J]. 中华中医药杂志,2011(4):841-843.
[7] 周新惠,赵荣华,张荣平,等. 三七不同加热炮制品中5种皂苷类成分的含量测定[J]. 云南中医学院学报,2013,36(6):11-14.
[8] 陈奇. 中药药理研究方法学[M]. 3版. 北京:人民卫生出版社,2011:518.
[9] Myers A, Papadopoulos A, O’Day D, et al. Sexual differentiation of arachidonate toxicity in mice[J]. Journal of Pharmacology and Experimental Therapeutics,1982,222(2):315-318.
[10] HECHLER B, NONNE C, ROH E J, et al. MRS2500
[2-iodo-N6-methyl-(N)-methanocarba-2’-deoxyadenosine-3’,5’-bisphosphate], a potent, selective, and stable antagonist of the platelet P2Y1 receptor with strong antithrombotic activity in mice[J]. J Pharmacol Exp Ther,2006,316(2):556-563.
[11] 杨涛,胡昌江,李文兵,等. 生、熟大黄对高分子右旋糖酐所致血瘀大鼠血液流变性的比较[J]. 中国实验方剂学杂志,2012(21):248-250.
[12] YAN T H, DU B S, YANG W, et al. Effects of salidroside on hemorheology and myocardial ischemic reperfusion injury in isolated heart of rats[J]. Chinese Journal of Natural Medicines,2008,6(3):219-222.
[13] 黄静,蒋伟哲,谭建宁,等. 姜精油对急性血瘀模型大鼠血液流变学的影响[J]. 中国药房,2012,23(11):969-971.
[14] 汪素娟,康安,狄留庆,等. 银杏叶提取物主要活性成分药动学研究进展[J]. 中草药,2013,44(5):626-631.
[15] 林伟鑫,姚曦,李勇,等. 三七总皂苷提取、大孔树脂纯化工艺研究[J]. 食品与药品,2015(3):156-161.
[16] 乔丽菲,孙承韬,王宏运,等. 复方栝楼桂枝汤的大孔树脂纯化工艺研究[J]. 江西中医药,2017,48(7):55-59.
[17] 徐冬英,潘会君. 三七止血作用的研究进展[J]. 现代医药卫生,2008,24(2):224-225.
[18] 周新惠,赵荣华,张荣平,等. 三七不同加热炮制品中5种皂苷类成分的含量测定[J]. 云南中医学院学报,2013,36(6):11-14.
[19] 李仪奎. 中药药理实验方法学[M]. 2版. 上海:上海科学技术出版社,2006:684-687.
[20] 武双,郭从亮,崔秀明,等. 炮制前处理对三七中三七皂苷含量的影响[J]. 中国医院药学杂志,2015,37(24):2208-2212.
[21] 孙思,陈亮,王鑫,等. 三七不同炮制品中抑制血管紧张素转化酶活性差异及人参皂苷Rg1和Rb1的含量测定[J]. 沈阳药科大学学报,2016,31(2):127-130.
[22] 轻曼古丽·阿吉,马虎,美合热阿依·伊萨克,等. 榅桲总黄酮对血栓形成和血小板聚集作用的影响[J]. 中成药,2015,37(7):1589-1592.
[23] WOLDHUIS B, TANGELDER G J, SLAAF D W, et al. Influence of dextrans on platelet distribution in arterioles and venules[J]. Pflügers Archiv,1993,425(3-4):191-198.
[24] 谢茵,邢桂琴,刘秀芬. 三七提取液中三七总皂苷的分离纯化工艺研究[J]. 山西医科大学学报,2006,37(6):613-615.

备注/Memo

备注/Memo:
收稿日期: 2018 - 11- 13
基金项目: 国家中药标准化项目(ZYBZH-Y-YN-44)
第一作者简介: 黄加文(1990-),男,助理工程师,研究方向:中药药理。
△通信作者: 王自梁,E-mail:zlwang3075@163.com
更新日期/Last Update: 2019-07-11