我们的网站为什么显示成这样?

可能因为您的浏览器不支持样式,您可以更新您的浏览器到最新版本,以获取对此功能的支持,访问下面的网站,获取关于浏览器的信息:

|本期目录/Table of Contents|

温肾化痰方对ApoE基因敲除小鼠动脉粥样硬化易损斑块模型Orai1、 Caspase、Bax、Bcl-2等基因及其相关蛋白表达的影响*(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2019年01期
页码:
8-12
栏目:
实验研究
出版日期:
2019-10-29

文章信息/Info

Title:
Effect of Wenshen Huatan Decoction on the Expression of Orai1, Caspase, Bax and Bcl-2 Genes and Related Proteins in Aulnerable Plaque Model of ApoE Knockout Mice
文章编号:
1000-2723(2019)01-0008-05
作者:
林海丹1陈铭泰2庄震坤1张忠2△刘彩如2
(1. 广州中医药大学第四临床医学院,广东 广州 510006;2. 广州中医药大学深圳市中医院心血管科,广东 深圳 518100;)
Author(s):
LIN Haidan1 CHEN Mingtai1 ZHUANG Zhenkun2 ZHANG Zhong1 LIU Cairu1
(1. Shenzhen Traditional Chinese Medicine Hospital of Guangzhou University of TCM, Shenzhen 518000, China;2. The Fourth Clinical Medical College of Guangzhou University of TCM, Guangzhou 510006, China)
关键词:
动脉粥样硬化 温肾化痰方 Orai1 Caspase Bax Bcl-2
Keywords:
atherosclerosis Wenshen Huatan decoction Orai1 Caspase Bax Bcl-2
分类号:
R285.5
DOI:
10.19288/j.cnki.issn.1000-2723.2019.01.002
文献标识码:
A
摘要:
目的观察温肾化痰方对ApoE基因敲除小鼠动脉粥样硬化易损斑块模型Orai1和Caspase3、Caspase9、Bax及Bcl-2等基因及其相关蛋白表达的影响。方法建立ApoE基因敲除小鼠动脉粥样硬化易损斑块模型,随机分成空白对照组、易损斑块组、阳性对照组、温肾化痰方低、中、高剂量组。13周后麻醉处死小鼠,采用RT-PCR技术检测主动脉平滑肌细胞Orai1、Caspase3、Caspase9、Bax及Bcl-2等基因的表达情况,并采用Western blot法检测主动脉窦Orai1、Caspase3、Caspase9、Bax及Bcl-2等蛋白表达变化。结果与空白对照组相比,易损斑块组Orai1、Caspase3、Caspase9、Bax mRNA及蛋白表达水平明显上升(P<0.01),而Bcl-2 mRNA及蛋白明显下降(P<0.01)。与易损斑块组相比,阳性对照组与温肾化痰方各剂量组均能下调上述基因(不包括Bcl-2)mRNA及蛋白表达水平,以及同时能上调Bcl-2的mRNA及蛋白表达,且中药剂量越高,调节效果越明显。与阳性对照组相比,温肾化痰方高剂量组下调Orail、Caspase3、Caspase9、Bax mRNA及蛋白水平以及上调Bcl-2 mRNA及蛋白水平的作用基本一致。这两组小鼠的上述基因及蛋白表达与空白对照组相比,统计无显著性差异。结论温肾化痰方能对应调节相关基因及蛋白表达从而干预易损斑块的形成及进展,进而起到保护心血管的作用。
Abstract:
Objective To observe the effects of Wenshen Huatan decoction on the expression of Orai1, Caspase3, Caspase9, Bax and Bcl-2 genes and their related proteins in atherosclerotic vulnerable plaque models of ApoE knockout mice. Methods The model of atherosclerotic vulnerable plaque in ApoE knockout mice was established and randomly divided into six groups, like blank control group, vulnerable plaque group, positive control group, Wenshen Huatan decoction low, medium and high dose groups. After 13 weeks, the mice were sacrificed by anesthesia. The expressions of Oria1, Caspase3, Caspase9, Bax and Bcl-2 genes in aortic smooth muscle cells were detected by RT-PCR technology. The expressions of protein associated with the above genes in the aortic sinus were also detected by Western blot technology. Results Compared with blank control group, the expression levels of Orai1, Casepase3, Casepase9 and Bax mRNA and protein in the vulnerable plaque group increased significantly(P<0.01), while the ones of Bcl-2 decreased significantly(P<0.01). Compared with the vulnerable plaque group, the mRNA and protein expression of the above genes(excluding Bcl-2) in the positive control group and Wenshen Huatan decoction group were down-regulated, and simultaneously the expression of Bcl-2’s mRNA and protein were up regulated. It’s also could be observed that the higher the dosage of the decoction, the more obvious the adjustment effect. Compared with the positive control group, the expression of those genes and protein in the high-dose group of Wenshen Huatan decoction were almost in the same. Among these two groups and blank control group, there was no statistically significant difference in the expression of those genes and proteins. Conclusion Wenshen Huatan decoction can prevent the formation and progression of vulnerable plaques by regulating the expression of relational genes and proteins, and thus protect the cardiovascular system.

参考文献/References

[1] HARTMAN J, FRISHMAN W H. Inflammation and atherosclerosis: a review of the role of interleukin-6 in the development of atherosclerosis and the potential for targeted drug therapy[J]. Cardiol Rev,2014,22 (3):147-151.
[2] FIN A V, NAKANO M, NARULA J, et al. Concept of vulnerable/unstable plaque[J]. Arterioscler Thromb Vasc Biol,2010,30(7):1282-1292.
[3] BEMA E A, WOODARD G E, ROSADO J A. Orais and STIMs:physiological mechanisms and disease[J]. J Cell Mol Med,2012,16(3):407-424.
[4] TREBAK M. STIM/Orai signaling complexes in vascular smooth muscle[J]. J Physiol,2012,590(17):4201-4208.
[5] WEI L L, CARRELL E M, BONCOMPAGNI S, et al. Orail-dependent calcium entry promotes skeletal muscle growth and limits fatigue[J]. Nat Commun,2013(4):2805.
[6] PENNA A, DEMURO A, YEROMIN A V, et al. The CRAC channel consists of a tetramer formed by stim-induced dimerization of orai dimmers[J]. Nature,2008,456(7218):116-120.
[7] 陈梅华. Orail-BK_(Ca)信号复合物调节血管平滑肌细胞收缩和血管张力的作用及其机制[D]. 合肥:安徽医科大学,2016.
[8] 田晋帆,葛长江,吕树铮,等. 基质交感分子1在载脂蛋白E基因敲除小鼠动脉粥样硬化斑块形成中的作用[J]. 中华老年心脑血管病杂志,2015,17(2):183-187.
[9] KONSTADOULAKIS M M, KYMIONIS G D, KARAGIANI M, et al. Evidence of apoptosis in human carotid atheroma[J]. J Vasc Surg,1998,27(4):733-739.
[10] 门保忠,周定标,石怀银,等,Bax 基因在人颈动脉粥样硬化斑块中的表达[J]. 军医进修学院学报,2010,31(6):594-596.
[11] 门保忠,周定标,石怀银. 颈动脉粥样硬化分子病理学研究进展[J]. 中国动脉硬化杂志,2004,12(3):370-372.
[12] VELA L, GONZALO O, NAVAL J, et al. Direct interaction of Bax and Bak proteins with Bcl-2 homology domain 3(BH3)-only proteins in living cells revealed by fluorescence complementation[J]. J Biol Chem,2013,288(7):4935-4946.
[13] 李录,贾绍斌,陈大鹏,等,bcl-2在同型半胱氨酸致血管内皮细胞凋亡中的作用[J]. 临床心血管病杂志,2014,30(1):75-78.
[14] LOOR G, KONDAPALLI J, IWASE H, et al. Mitochondrial oxidant stress trig-gers cell death in simulated ischemia-reperfusion[J]. Biochim Bio-phys Acta,2010, 1813(7):1382-1394.
[15] 程修平,宫丽鸿. 稳斑汤对ApoE基因敲除小鼠主动脉粥样硬化不稳定斑块形成Bax和Bcl-2 mRNA的影响[J]. 世界中西医结合杂志,2015,10(12):1728-1731.
[16] 管耘园,叶炳华,卢辉和,等. 卡维地洛干预对转染p53基因家兔颈动脉粥样硬化斑块稳定性影响的研究[J]. 中华心血管病杂志,2007,35(1):63-68.
[17] TSUJIMOTO Y, FINGER LR, YUNIS J, et al. Cloning of the chromosome breakpoint of neoplastic B cells with the t(14:18) chromosome translocation[J]. Science,1984, 226(4678):1097-1099.
[18] 德乐黑巴特尔,达林泰,赵诚. YAP和Caspase在恶性肿瘤中的作用机制及其关联的研究进展[J]. 内蒙古医学杂志,2012,44(5):575-579.
[19] SIEST G, PILLOT T, R?魪GIS B A, et al. Apolipoprotein E: an important gene and protein to follow in laboratory medicine[J]. Clin Chem,1995,41(8 Pt1):1068-1086.
[20] SKOOG I. Vascular aspects in Alzheimer′s disease[J]. J Neural Transm Suppl,2000,59:37-43.
[21] 石佳鹭,张莉. 载脂蛋白E基因多态性与血脂及动脉粥样硬化相关性研究进展[J]. 现代医药卫生,2018,34(7):1032-1035.

备注/Memo

备注/Memo:
收稿日期: 2019 - 01- 15
* 基金项目: 国家自然科学基金(81573922);深圳市科技创新委员会项目(JCYJ20160428181826351);深圳市三名工 程——中国医学科学院阜外医院张健教授心血管病团队(SZSM201612033)
第一作者简介: 林海丹(1991-),女,在读硕士研究生,医师,研究方向:中西医结合治疗心血管疾病。
△通信作者: 张忠,E-mail:zzicu@126.com
更新日期/Last Update: 2019-10-22