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五谷麒麟膏调控Bcl-xl/Bak促进肛瘘术后创面愈合机制研究*(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2019年01期
页码:
13-18
栏目:
实验研究
出版日期:
2019-10-29

文章信息/Info

Title:
Study on the Mechanism of Regulating Bcl-xl/Bak and Promoting Wound Healing after Anal Fistula by Wugu Kirin Ointment
文章编号:
1000-2723(2019)01-0013-06
作者:
苗咪1冯文哲2△阴新元1柴小琴2朱建东1
(1. 陕西中医药大学,陕西 咸阳 712000;2. 陕西中医药大学附属医院,陕西 咸阳 712000)
Author(s):
MIAO Mi1FENG Wenzhe2 YIN Xinyuan1 CHAI Xiaoqin2 ZHU Jiandong1
(1. Fujian Academy of Traditional Chinese Medicine, Fuzhou 350003, China;
关键词:
五谷麒麟膏 肛瘘术后创面 Bcl-xl/Bak 细胞凋亡 创面愈合
Keywords:
Wugu Kirin ointment wound healing after anal fistula Bcl-xl/Bak apoptosis wound healing
分类号:
R285.5
DOI:
10.19288/j.cnki.issn.1000-2723.2019.01.003
文献标识码:
A
摘要:
目的观察五谷麒麟膏对大鼠感染创面组织Bcl-xl/Bak的调控作用,探讨五谷麒麟膏促进肛瘘术后创面愈合的细胞凋亡机制。方法选取SD大鼠,除空白组外其余大鼠制备为全层皮肤缺损模型,创面淋洒大鼠粪便混悬液造成急性感染创面。造模成功后观察各组大鼠基本情况、创面愈合率,并检测创面肉芽组织中新生毛细血管、细胞凋亡率及Bcl-xl/Bak的比值。结果用药后第1周实验组大鼠的基本情况良好,京万红组及模型组欠佳,此后无明显差异;治疗后第3、7、11天实验组大鼠创面愈合率、细胞凋亡率均高于模型,治疗后第15天实验组细胞凋亡率低于模型组;实验组Bcl-xl/Bak的比值低于模型组;京万红组与低浓度组无统计学差异。结论五谷麒麟膏可以通过降低Bcl-xl/Bak的比值,抑制创面肉芽组织细胞的过度凋亡,促进肛瘘术后创面的愈合,其中以高浓度组效果最佳。
Abstract:
Objective To observe the regulatory effect of Wugu Kirin ointment on Bcl-xl/Bak in infected wound tissue of rats, and to explore the apoptosis mechanism of Wugu Kirin ointment in promoting wound healing after anal fistula. Methods SD rats were selected and the other rats except the blank group were made into full-thickness skin defect model. The acute infected wounds were caused by drenching the fecal suspension of the rats. After successful establishment of the model, the basic situation, wound healing rate, new capillaries, apoptosis rate and Bcl-xl/Bak ratio in granulation tissue of each group were observed. Results In the first week after treatment, the basic condition of the rats in the experimental group was good, while that in the Jingwanhong group and the model group was not good, but there was no significant difference since then. On the 3rd, 7th and 11th day after treatment, the wound healing rate and apoptosis rate of the experimental group were higher than those of the model group, and the apoptosis rate of the experimental group was lower than that of the model group on the 15th day after treatment, and the ratio of Bcl-xl/Bak in the experimental group was lower than that of the model group. There was no significant difference between Jingwanhong group and low concentration group. Conclusion Wugu Kirin ointment can inhibit the excessive apoptosis of granulation tissue cells and promote the wound healing after anal fistula by reducing the ratio of Bcl-xl/Bak, and the high concentration group has the best effect.

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备注/Memo

备注/Memo:
收稿日期: 2019 - 01- 12
* 基金项目: 咸阳市科学技术研究计划项目(2018k02-97)
第一作者简介: 苗咪(1994-),女,在读硕士研究生,研究方向:中医肛肠疾病的临床及实验研究。
△通信作者: 冯文哲,E-mail:szfygck@163.com
更新日期/Last Update: 2019-10-22