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推拿对膝骨性关节炎大鼠滑膜TRAF6、 NF-κBp65 mRNA及蛋白的影响*(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2019年01期
页码:
24-29
栏目:
实验研究
出版日期:
2019-10-29

文章信息/Info

Title:
Effects of Massage on the Expression of TRAF6 and NF-κBp65 mRNA and Protein in Synovium of Rats with Knee Osteoarthritis
文章编号:
1000-2723(2019)01-0024-06
作者:
曲崇正1刘姣2陶静1江钢辉1
(1. 广州中医药大学第三附属医院,广东 广州 510360;2. 广州中医药大学,广东 广州 510405)
Author(s):
QU Chongzheng1 LIU Jiao2 TAO Jing1 JIANG Ganghui1
(1. The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510360, China;2. Guangzhou University of Chinese Medicine, Guangzhou 510405, China)
关键词:
膝骨性关节炎 推拿 TRAF6 NF-κBp65 大鼠
Keywords:
knee osteoarthritis massage TRAF6 NF-ΚBp65 rats
分类号:
R244.1
DOI:
10.19288/j.cnki.issn.1000-2723.2019.01.005
文献标识码:
A
摘要:
目的对建立的大鼠膝骨性关节炎模型行推拿治疗,观察其滑膜TRAF6、NF-κBp65 mRNA及蛋白表达,探讨推拿治疗膝骨性关节炎的效果及其机制。方法采用随机化的原则,抽取10只大鼠为空白组,余下40只大鼠采用木瓜蛋白酶关节注射建立大鼠膝骨性关节炎模型,待造模成功后,再将造模大鼠随机分为模型组、推拿组、灌胃组、推拿加灌胃组,每组10只。空白组常规饲养,推拿组一指禅手法治疗,灌胃组塞来昔布灌胃,推拿加灌胃组予以上两种治疗。治疗4周后取材,对膝关节滑膜行荧光定量PCR、Western blot检测TRAF6、NF-κBp65 mRNA和蛋白表达水平。结果①荧光定量PCR结果显示,与空白组相比,模型组、推拿组、灌胃组的TRAF6、NF-κBp65 mRNA表达均上调,差异具有统计学意义(P﹤0.05),推拿加灌胃组与空白组相比TRAF6、NF-κBp65 mRNA表达几乎无差异,稍上调(P>0.05);与模型组相比,各治疗组TRAF6、NF-κBp65 mRNA表达下调,差异具有统计学意义(P﹤0.05),且各治疗组TRAF6、NF-κBp65 mRNA表达量:推拿加灌胃组﹤推拿组﹤灌胃组,差异具有统计学意义(P﹤0.05)。②Western blot结果显示,与空白组相比,其余各组TRAF6、NF-κBp65蛋白的表达上调,差异具有统计学意义(P﹤0.05);与模型组相比,其余各组TRAF6、NF-κBp65蛋白的表达下调,差异具有统计学意义(P﹤0.05),且各治疗组TRAF6、NF-κBp65蛋白表达量:推拿加灌胃组﹤推拿组﹤灌胃组,差异具有统计学意义(P﹤0.05)。结论推拿通过下调膝骨性关节炎大鼠滑膜组织TRAF6、NF-κBp65 mRNA和蛋白的表达,减轻滑膜炎症反应,从而起到消炎止痛的作用,最终达到防治膝骨性关节炎的目的。
Abstract:
Objective To observe the expression of TRAF6, NF-p65 mRNA and protein in synovium of knee osteoarthritis rat model by massage, and to explore the effect and mechanism of massage on knee osteoarthritis. Methods Ten rats were randomized into blank group and the remaining 40 rats were injected with papain to establish knee osteoarthritis model. After the model was successfully established, the rats were randomly divided into model group, massage group, gastric lavage group and massage plus gastric lavage group, with 10 rats in each group. The blank group was fed routinely, the massage group was treated with one finger Zen manipulation, the gastric lavage group was treated with celecoxib, and the massage plus gastric lavage group was treated with the above two methods. After 4 weeks of treatment, the synovium of knee joint was detected by fluorescence quantitative PCR and Western blot for TRAF6, NF-p65 mRNA and protein expression. Results ① The results of fluorescence quantitative PCR showed that compared with the blank group, the expression of TRAF6 and NF-p65 mRNA in model group, massage group and gastric lavage group were up-regulated, with significant difference(P<0.05). Compared with the blank group, the expression of TRAF6 and NF-p65 mRNA in massage plus gastric lavage group was almost no difference, but slightly up-regulated(P>0.05). Compared with the model group, the expression of TRAF6 and NF-p65 mRNA in each treatment group was up-regulated. The difference was statistically significant(P<0.05), and the expression of TRAF6 and NF-p65 mRNA in each treatment group was significantly different(P<0.05). ② Western blot results showed that the expression of TRAF6 and NF-p65 protein was up-regulated in other groups compared with the blank group(P<0.05). Compared with the model group, the expression of TRAF6 and NF-p65 protein was down-regulated in the other groups(P<0.05). The expression of TRAF6 and NF-p65 protein in each treatment group was significantly different(P<0.05). The expression of TRAF6 and NF-p65 protein in massage plus gastric lavage group was lower than that in massage. The difference was statistically significant(P<0.05). Conclusion It is suggested that massage can decrease the expression of TRAF6、NF-p65 mRNA and protein in synovial tissue of rats with knee osteoarthritis and alleviate the inflammatory reaction of synovium, thus playing an anti-inflammatory and analgesic role, and ultimately achieving the goal of preventing and treating knee osteoarthritis.

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备注/Memo

备注/Memo:
收稿日期: 2019 - 02- 05
* 基金项目: 高水平大学建设(A1-AF01816Z0207)
第一作者简介: 曲崇正(1974-),男,博士,副主任中医师,研究方向:中医筋伤学疾病、骨关节病、骨质疏松症临床防 治研究。E-mail:873397214@qq.com
更新日期/Last Update: 2019-10-22