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平喘宁调节PI3K/Akt信号通路干预寒性哮喘大鼠气道重塑的机制*(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2019年02期
页码:
11-15
栏目:
实验研究
出版日期:
2019-11-15

文章信息/Info

Title:
Effect of Pingchuanning on PI3K/Akt Signaling Pathway in Airway Remodeling in Cold Asthmatic Rats
文章编号:
1000-2723(2019)02-00011-05
作者:
李明明1屈 彬1方向明12△
(1. 安徽中医药大学,安徽 合肥 230038;2. 中药复方安徽省重点实验室,安徽 合肥 230038)
Author(s):
LI Mingming1 QU Bin1 FANG Xiangming12
(1. Anhui University of Chinese Medicine, Hefei 230038, China;2. Anhui Provincial Key Laboratory of Traditional Chinese Medicine Compound, Hefei 230038, China)
关键词:
平喘宁 ASK1 TSC1 哮喘 气道重塑 大鼠
Keywords:
Pingchuanning ASK1 TSC1 asthma airway remodeling rats
分类号:
R285.5
DOI:
10.19288/j.cnki.issn.1000-2723.2019.02.002
文献标识码:
A
摘要:
目的观察平喘宁对PI3K-Akt/PKB信号通路中的相关蛋白的干预探讨哮喘气道重塑的机制。方法 将105只雄性SD大鼠按随机数法分成正常组、模型组、平喘宁高剂量组、平喘宁中剂量组、平喘宁低剂量组、桂龙咳喘宁组以及地塞米松组,每组15只。采用HE染色法观察大鼠肺组织的病理改变;采用RT-qPCR检测ASK1、TSC1的表达量。结果 HE染色后,相比于正常组,模型组大鼠支气管结构发生改变,可见多种炎性细胞浸润、粘液栓及气管平滑肌增生,而药物组均有不同程度的改善。RT-qPCR检测:相较于正常组,模型组ASK1上调的幅度最为显著(P<0.01);对比于模型组,平喘宁高剂量组对ASK1的表达量下调最为显著并且优于其余药物组(P<0.01)。相较于正常组,模型组下调TSC1的幅度最为显著(P<0.01);与模型组相比,平喘宁高剂量组对TSC1的表达量的上调最为显著,(P<0.01),而此组对TSC1表达量上调又高于其余药物治疗组(P<0.01)。结论 平喘宁可以通过对寒哮大鼠PI3K-Akt/PKB信号传导通路中的ASK1、TSC1调节进而减缓寒哮大鼠的气道炎症反应,减低气道的高反应性,并能够缓解气道重塑达到哮喘治疗的效果。
Abstract:
Objective To observe the intervention of Pingchuanning on the related proteins in PI3K-Akt/PKB signaling pathway to explore the mechanism of airway remodeling in asthma. Methods A total of 105 male Sprague-Dawley rats were randomly divided into normal group, model group, Pingchuanning high-dose group, Pingchuanning middle-dose group, Pingchuanning low-dose group, Guilong Kechuanning group and ground plug. Rice pine group, 15 in each group. The pathological changes of rat lung tissues were observed by HE staining. The expression levels of ASK1 and TSC1 were detected by RT-qPCR. Results After HE staining, the bronchial structure of the model group was changed compared with the normal group. A variety of inflammatory cell infiltration, mucus plug and tracheal smooth muscle hyperplasia were observed, and the drug group had different degrees of improvement. RT-qPCR detection: Compared with the normal group, the amplitude of ASK1 up-regulation was the most significant in the model group(P<0.01). Compared with the model group, the expression of ASK1 was lower in the high-dose group of Pingchuanning than in the other groups(P<0.01). Compared with the normal group, the model group had the most significant down-regulation of TSC1(P<0.01). Compared with the model group, the high-dose group of Pingchuanning had the most significant up-regulation of TSC1 expression(P<0.01). The up-regulation of TSC1 expression was higher in the group than in the middle and low-dose groups (P<0.01). Conclusion Pingchuanning can alleviate airway inflammation in rats with cold asthma by regulating ASK1 and TSC1 in PI3K-Akt/PKB signaling pathway in rats with cold asthma, reduce airway hyperresponsiveness, and relieve gas. Road remodeling achieves the effect of asthma treatment.

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备注/Memo

备注/Memo:
收稿日期: 2019 - 04- 03
* 基金项目: 安徽省高校自然科学研究重点项目(KJ2018A0281)
第一作者简介: 李明明(1991-),女,在读硕士研究生,研究方向:方剂配伍规律与作用机制研究。
△通信作者: 方向明,E-mail:fxm.bsh@163.com
更新日期/Last Update: 2019-11-19