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基于网络药理学探讨丹栀逍遥散治疗多囊卵巢综合征 的物质基础及作用机制*(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2019年04期
页码:
69-75
栏目:
方药研究
出版日期:
2020-05-18

文章信息/Info

Title:
Exploring the Effective Components and Mechanism of Danzhi Xiaoyao Powder in Treatment of PCOS Based on Network Pharmacology
文章编号:
1000 - 2723(2019)04- 0069 - 07
作者:
汪碧云1伍庭凤2李舒婷1谢琳玲1曾 蕾3△
(1. 广州中医药大学第一临床医学院,广东 广州 510405;2. 广州中医药大学附属中山中医院,广东 中山 528400;3. 广州中医药大学第一附属医院,广东 广州 510405)
Author(s):
WANG Biyun1WU Tingfeng2LI Shuting1XIE Linling1ZENG Lei3
(1. The First Clinical Medicine College of Guangzhou University of Chinese Medicine, Guangzhou 510405, China;2. Zhongshan Hospital of Traditional Chinese Medicine Affiliated to Guangzhou Universityof Chinese Medicine, Zhongshan 528400, China;3. The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China)
关键词:
网络药理学多囊卵巢综合征丹栀逍遥散作用机制
Keywords:
network pharmacologyPCOSDanzhi Xiaoyao Powdermechanism
分类号:
R285.5
DOI:
10.19288/j.cnki.issn.1000-2723.2019.04.012
文献标识码:
A
摘要:
目的通过网络药理学方法,预测丹栀逍遥散各药物活性成分对多囊卵巢综合征的作用靶点,探讨丹栀逍遥散(DZXYP)治疗多囊卵巢综合征(PCOS)的潜在作用机制。方法 通过TCMSP数据库筛选出丹栀逍遥散中各药物的主要活性成分,并使用PubChem数据库和 SwissTarget Prediction预测相关作用靶点。利用GeneCards挖掘已知的PCOS基因靶点。结合药物的预测靶点及疾病靶点导入String数据库构建药物--疾病靶点蛋白互作网络,最后采用 DAVID数据库和Cytoscape对关键节点进行生物功能及代谢通路分析。结果 丹栀逍遥散8味药共筛选出142个活性成分,预测出483个相关靶点;经过数据检索得到PCOS相关靶点3 619个,与药物有140个交集。对核心靶点共富集出286个生物学过程(BP)、81个分子功能(MF)和34个细胞成分(CC),以及83条KEGG通路。结论 丹栀逍遥散可能是通过对多个靶点作用,调控磷脂酰肌醇3激酶 /蛋白激酶B(PI3K/Akt)、卵巢类固醇激素合成、胰岛素抵抗(IR)等多个信号通路,改善PCOS的炎症状态、恢复胰岛素敏感性以及降低雄激素的合成起到治疗PCOS的作用,为丹栀逍遥散的作用机制提供了新的阐释以及为进一步开展其药理研究提供新思路。
Abstract:
Objective To predict the potential target of Danzhi Xiaoyao Powder(DZXYP) treatment of Polycystic Ovary Syndrome(PCOS) by the pharmacological method of network, so as to explore the pharmacokinetic mechanism of the DZXYD. Methods The main active components of DZXYD were screened by TCMSP database, and the relevant targets were predicted using PubChem database and SwissTarget Prediction. Gene Cards are used to mine known PCOS gene targets. Combined with the drug’s predicted target and disease target, the String database was constructed to construct the drug-disease target protein interaction network. Finally, the DAVID database and Cytoscape were used to analyze the biological functions and metabolic pathways of key nodes. Results A total of 154 active components were screened by DZXYP, and 483 related targets were predicted. After data retrieval, 3 619 PCOS-related targets were obtained, and there were 140 intersections with drugs. A total of 286 biological processes(BP) , 81 molecular functions(MF) , 34 cellular components(CC) and 83 KEGG pathways were enriched for key targets. Conclusion DZXYP may act on multiple targets, regulate multiple signaling pathways such as phosphatidylinositol 3-kinase/protein kinase B(PI3K/Akt), ovarian steroid hormone synthesis, insulin resistance(IR), improve the inflammatory state of PCOS, restored insulin Sensitivity and reducted the synthesis of androgen. Thereby playing a role in the treatment of PCOS. It provides a new interpretation of the mechanism of action of DZXYP and provides new ideas for further research on its pharmacology.

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备注/Memo

备注/Memo:
收稿日期: 2019 - 07- 18
* 基金项目: 国家自然科学基金项目(81804135)
第一作者简介: 汪碧云(1994-),女,在读硕士研究生,研究方向:中西医结合防治妇科疾病。
△通信作者: 曾蕾, E-mail:407687448@qq.com
更新日期/Last Update: 2020-06-04