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调督针刺对阿尔茨海默病大鼠学习记忆能力的改善作用*(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2020年01期
页码:
7-11
栏目:
实验研究
出版日期:
2020-07-10

文章信息/Info

Title:
Effect of Tiaodu Acupuncture on Learning and Memory Ability of Rats with Alzheimer’s Disease
文章编号:
1000 - 2723(2020)01- 0007 - 05
作者:
孙兴华张 淼武文鹏李书霖
(黑龙江中医药大学附属第二医院,黑龙江 哈尔滨 150001)
Author(s):
SUN Xinghua ZHANG Miao WU Wenpeng LI Shulin
(The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, 150001, China)
关键词:
调督针刺阿尔茨海默病炎性因子学习记忆能力
Keywords:
Tiaodu acupuncture alzheimer’s disease inflammatory factors learning and memory ability
分类号:
R245
DOI:
10.19288/j.cnki.issn.1000-2723.2020.01.002
文献标识码:
A
摘要:
目的探讨调督针刺对阿尔茨海默病(Alzheimer’s disease,AD)大鼠学习记忆能力的改善作用。方法 将雄性SD大鼠48只,按随机数字表法随机分为4组,即:空白组、假手术组、模型组、针刺组,每组12只。采用双侧海马注射Aβ1-42 5 μL制备AD大鼠模型。造模成功后第8天开始,针刺组取“百会”“风府”针刺,并接通脉冲针灸治疗仪[疏波(频率为2 Hz)]留针30 min,每日治疗1次,连续治疗28 d。采用Morris水迷宫试验检测大鼠学习记忆能力,监测各组大鼠体质量情况,光镜下观察海马病理组织形态学,采用免疫组织化学法检测海马组织白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、胶质纤维酸性蛋白(GFAP)的表达。结果 与模型组比较,针刺组大鼠体质量增长较快(P<0.05);与模型组比较,针刺组大鼠潜伏期明显缩短(P<0.05),游泳距离明显缩短(P<0.05),神经细胞排列较为规则、神经元萎缩较少,IL-6、TNF-α、GFAP表达明显减少(P<0.05)。结论 调督针刺能够改善AD模型大鼠学习、记忆能力,减轻炎症反应,但相关的分子调控机制仍需要进一步研究。
Abstract:
Objective To observe the effect of Tiaodu acupuncture on improving the learning and memory ability of Alzheimer’s disease(AD) rats. Methods 48 male SD rats were selected and divided into 4 groups by random number table, including blank group, sham operation group, model group and acupuncture group, each group with 12 rats. AD models were prepared by injecting Aβ1-42 5 μL in both hippocampus. Starting from the 8 d after successfully modeling, the acupuncture group was given acupuncture therapy on the Baihui and Fengfu, and connected the pulse acupuncture treatment instrument [Shubo(frequency: 2 Hz)] for 30 min, once a day, treated for 28 d. Morris water maze was used to test the learning and memory ability of rats and the weight were monitored. The pathomorphology of hippocampus were observed under light microscope and the expressions of IL-6, TNF-α and GFAP were detected by immunohistochemistry. Results Compared with the model group, the weight of rats in acupuncture group increased rapidly(P<0.05), the latency and swimming distance of the acupuncture group were significantly shortened(P<0.05), the arrangement of nerve cells were regularly, and the number of neurons atrophy were less, also the expressions of IL-6, TNF-α, GFAP were significantly reduced(P<0.05). Conclusion Tiaodu acupuncture could improve the learning and memory ability of AD model rats, and reduce the inflammatory response, however the related molecular mechanism need further study.

参考文献/References

[1] 雷曦,王健辉,程肖蕊,等. 防治阿尔茨海默病多靶点药物研究进展[J]. 国际药学研究杂志,2016,43(2):205-215. [2] 李虹霖,徐飞,夏昆鹏,等. 头穴丛刺对AD大鼠cAMP-PKAc-CREB及胆碱能的影响[J]. 针灸临床杂志,2016,32(11):81-84. [3] 董贤慧,柴锡庆. 阿尔茨海默病发病机制研究进展[J]. 中国老年学杂志,2014,34(20):5906-5912. [4] 彭英,李萍萍,李琳,等. 抗阿尔茨海默病药物临床研究进展[J]. 药学学报,2016,51(8):1185-1195. [5] 董晓红,姚壮,张艳,等. 通天草治疗阿尔茨海默症的药效物质研究[J]. 中医药信息,2017,34(4):1-3. [6] 曲岩,王佳志,李文鹏,等. 补阳还五汤中有效成分对 AD 细胞模型的影响的实验研究[J]. 湖北中医药大学学报,2017,19(1):11-15. [7] 钟高堂,魏亮,付德安. 复方海蛇胶囊联合多奈哌齐对阿尔茨海默病生化指标的影响[J]. 云南中医学院学报,2018,41(2):22-25. [8] 武峻艳,王杰,张俊龙. 从督脉论治不同脑老化疾病异病同治的理论基础-以阿尔茨海默病和帕金森病为例[J]. 中国针灸,2015,35(5):489-492. [9] 姜卓,宋琳,朴钟源,等. 地黄饮子组成药物的中药有效成分治疗AD研究进展[J]. 中医药学报,2017,45(5):93-98. [10] 李晓艳,刘双岭,单媛莉,等. 高维滨教授夹脊电针方法治疗神经根型颈椎病的临床研究[J]. 中国伤残医学,2015(10):53-54. [11] DOMINGUES C,DA CRUZ E SILVA O A B,HENNQUES A G,et al. Impact of cytokines and chemokines on Alzheimer’s disease neuropathological hallmarks[J]. Curr Alzheimer Res,2017,14(8):870-882. [12] 费洪新,高音,孙丽慧,等. 蝙蝠葛酚性碱对阿尔茨海默病模型小鼠学习记忆和海马的影响[J]. 中国实验方剂学杂志,2015,21(7):91-94. [13] FREIR D B,HOLSEHER C,HERRON C E. Blockade of long-term potentiation by beta-amyloid peptides in the CA1 region of the rat hippocampus in vivo[J]. J Neurophysiol,2001,85(2):708-713. [14] 冯荣芳,冯亚青,吕佩源. 阿尔茨海默病动物模型研究进展[J]. 脑与神经疾病杂志,2008,16(6):724-726. [15] 华兴邦,周浩良. 大鼠穴位图谱的研制[J]. 实验动物与动物实验,1991(1):1-5. [16] JIA J,WEI C,CHEN S,et al. The cost of Alzheimer’s diseasein China and re-estimation of costs worldwide[J]. Alzheimers Dement,2018,14(4):483-491. [17] 邓姣,韦益飞,何堃,等. 宽筋藤有效部位对D-半乳糖+Aβ25-35所致AD大鼠药效学研究[J]. 中药新药与临床药理,2019,30(5):553-559. [18] 董雯,王蓉. 阿尔茨海默病动物模型研究进展[J]. 中国现代神经疾病杂志,2015,15(8):610-614. [19] 孙博,费洪新,姜波,等. 阿尔茨海默病发病机制初探[J]. 中医药信息,2014,31(3):26-29. [20] 王渊,刘智斌,牛文民,等. “嗅三针”对AD小鼠海马iNOS和磷酸化tau蛋白表达的影响[J]. 陕西中医药大学学报,2018,41(5):102-106. [21] 张静爽,王蓉. 阿尔茨海默病发生机制的研究进展[J]. 首都医科大学学报,2014,35(6):721-724. [22] COSTELLO D A,CARNEY D G,LYNCh M A. α-TLR2 antibody attenuates the Aβ-mediated inflammatory response in microglia through enhanced expression of SIGIRR[J]. Brain Behav Immun,2015,46:70-79. [23] JIAO S S,YAO X Q,LIU Y H,et al. Edaravone alleviates Alzheimer’s disease-type pathologies and cognitive deficits[J]. Proc Natl Acad Sci U S A,2015,112(16):5225-5230. [24] 胡海燕,陈志裕,徐冬梅,等. 清心开窍方3种提取部位改善阿尔茨海默病大鼠学习记忆能力的机制研究[J]. 中国中西医结合杂志,2015,35(5):595-602. [25] 亓珅,杜怡峰. 炎性反应在阿尔茨海默病发病机制中作用的研究进展[J]. 中国神经免疫学和神经病学杂志,2013,20(4):278-280.

备注/Memo

备注/Memo:
收稿日期: 2019 - 12- 23
* 基金项目: 黑龙江省自然科学基金项目(LH2019H047);黑龙江省卫生计生委科研项目(2017-576)
第一作者简介: 孙兴华(1982-),男,主治医师,研究方向:中药临床药理学研究及针灸防治神经系统疾病临床、基础研究。
△通信作者: 李书霖,E-mail:1156789999@qq.com
更新日期/Last Update: 2020-07-20