我们的网站为什么显示成这样?

可能因为您的浏览器不支持样式,您可以更新您的浏览器到最新版本,以获取对此功能的支持,访问下面的网站,获取关于浏览器的信息:

|本期目录/Table of Contents|

逍遥散及其功效拆方对慢性铁过载诱导的肝纤维化的影响(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2021年01期
页码:
17-23
栏目:
实验研究
出版日期:
2021-06-28

文章信息/Info

Title:
Effects of Xiaoyao Powder and Its Efficacy Decomposed Recipes on Hepatic Fibrosis Induced by Chronic Iron Overload
文章编号:
1000-2723(2021)01-0017-07
作者:
周 薏葛冰景肖铁刚曹红燕戴彦成阙任烨
(上海中医药大学附属上海市中西医结合医院脾胃病科,上海 200082)
Author(s):
ZHOU Yi GE Bingjing XIAO Tiegang CAO Hongyan DAIYancheng QUE Renye
(Department of Gastroenterology, Shanghai TCM-integrated Hospital Affiliated Shanghai University of Traditional Chinese Medicine, Shanghai 200082,China)
关键词:
逍遥散肝纤维化铁过载功效拆方右旋糖酐铁
Keywords:
Xiaoyao Powder liver fibrosis iron overload efficacy decomposed recipes iron dextran
分类号:
R285.5
DOI:
10.19288/j.cnki.issn.1000-2723.2021.01.002
文献标识码:
A
摘要:
目的通过制作慢性铁过载诱导的大鼠肝纤维化模型,观察逍遥散对肝纤维化的改善作用及对体内铁代谢过程的影响,并通过功效拆方研究,探索逍遥散中疏肝药、健脾药、养血药在整方疗效中的作用及地位。方法 通过腹腔注射右旋糖酐铁50 mg/kg/d,连续7周制作慢性铁过载诱导的大鼠肝纤维化模型,药物干预在造模同时每日通过灌胃的方式给予逍遥散全方、逍遥散去疏肝药、逍遥散去健脾药、逍遥散去养血药、去铁胺(DFO)。使用全自动生化分析仪检测血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、血清铁(SI)、铁蛋白(SF)水平;肝组织进行病理组织学检测,包括HE染色、Masson染色、苦味酸-天狼星红染色等;免疫组化检测肝组织α-平滑肌肌动蛋白(α-SMA)、I型胶原蛋白(COL-1)的表达水平;采用火焰原子吸收光谱法检测大鼠肝组织铁含量。结果 与对照组比较,模型组大鼠肝功能ALT、AST明显升高,病理切片HE染色符合肝纤维化镜下表现,Ishak纤维化评分显著升高,Masson染色、苦味酸-天狼星红染色可见胶原纤维沉积明显,免疫组化结果提示肝组织内α-SMA、COL-1表达显著上升,血清铁、铁蛋白、肝脏总铁含量均显著升高。与模型组比较,逍遥散全方及各功效拆方组可明显降低大鼠肝功能ALT、AST,改善病理过程如Ishak评分及胶原染色半定量等均显著下降,肝组织α-SMA、COL-1表达显著降低,血清铁、铁蛋白、肝脏总铁含量亦明显下降,阳性对照药物去铁胺结果与逍遥散全方相仿。与逍遥散全方相比,逍遥散各功效拆方组均较整方疗效有显著降低。结论 逍遥散可显著改善慢性铁过载诱导的大鼠肝纤维化,其方中疏肝药、健脾药、养血药均对改善铁过载及纤维化有一定作用。推测逍遥散抑制铁过载维持铁稳态的作用可能是其疏肝、健脾、养血功效的核心机制。
Abstract:
Objective To observe the effect of Xiaoyao Powder on the improvement of liver fibrosis and iron metabolism in rats induced by chronic iron overload, and to explore the role and status of liver soothing, spleen strengthening and blood nourishing drugs in Xiaoyao Powder. Methods Rat liver fibrosis model induced by chronic iron overload was established by intraperitoneal injection of iron dextran(50 mg/kg/d) for 7 weeks. At the same time, the whole prescription of Xiaoyao Powder, or removing Liver Soothing drugs, spleen strengthening drugs, blood nourishing drugs and deferoxamine(DFO) were given daily by gavage. The levels of serum alanine aminotransferase(ALT), aspartate aminotransferase (AST), serum iron (SI) and ferritin (SF) were detected by automatic biochemical analyzer liver tissues were detected by histopathology, including HE staining, Masson staining, picric acid Sirius red staining, etc. immunohistochemistry was used to detect α-smooth muscle actin (α-SMA) and type I collagen protein (COL-1) expression. The content of liver iron in rat was detected by flame atomic absorption spectrometry. Results Compared with the control group, ALT and AST of liver function in the model group were significantly higher than those in the control group. The HE staining of pathological sections was consistent with the microscopic manifestations of liver fibrosis. The Ishak fibrosis score was significantly increased. Masson staining and picric acid Sirius red staining showed obvious collagen fiber deposition. Immunohistochemical results showed that the expression of α-SMA and COL-1 in liver tissue was significantly increased. Serum iron, ferritin and total liver iron were also significantly increased. Compared with the model group, the whole Xiaoyao powder recipe and its efficacy decomposed recipes could significantly reduce the ALT and AST of liver function, improve the pathological process, such as Ishak score and semi quantitative staining of collagen. The expressions of α-SMA and COL-1 in liver tissue were significantly decreased, and the contents of serum iron, ferritin and total liver iron were also significantly decreased. The results of positive control drug deferoxamine were similar to those of Xiaoyao Powder. Compared with the whole Xiaoyao Powder prescription, efficacy decomposed recipes in each group was significantly lower than that in the whole formula. Conclusion Xiaoyao Powder can significantly improve liver fibrosis induced by chronic iron overload in rats, and the drugs of soothing liver, strengthening spleen and nourishing blood have certain effects on improving iron overload and fibrosis. It is speculated that the effect of Xiaoyao Powder on inhibiting iron overload and maintaining iron homeostasis may be the core mechanism of its functions of soothing liver, strengthening spleen and nourishing blood.

参考文献/References

[1] AREZZINI B,LUNGHI B,LUNGARELLA G,et al. Iron overload enhances the development of experimental liver cirrhosis in mice[J]. Int J Biochem Cell Biol,2003,35(4):486-495.
[2] MUELLER S,AFDHAL N H,SCHUPPAN D. Iron,HCV,and liver cancer:hard metal setting the pace?[J]. Gastroenterology,2006,130(7):2229-2234.
[3] ALEXANDER J,TUNG B Y,CROGHAN A,et al. Effect of iron depletion on serum markers of fibrogenesis,oxidative stress and serum liver enzymes in chronic hepatitis C:results of a pilot study[J]. Liver Int,2007,27(2):268-273.
[4] 王庆生,李绍民,冯澜. 恩替卡韦联合逍遥散对肝纤维化肿瘤坏死因子TNF-α的影响[J]. 黑龙江医药科学,2016,39(1):84-86.
[5] 陈曦,牟璐璐,陈丹丹,等. 逍遥散对肝纤维化大鼠模型抗纤维化作用及其机制研究[J]. 中药新药与临床药理,2014,25(3):241-244.
[6] 李绍民,代立娟,冯澜,等. 逍遥散含药血清对人肝星形细胞分泌MMP-1和TIMP-1的影响[J]. 时珍国医国药,2016,27(9):2134-2136.
[7] ZHANG Y,ZHANG Y Y,XIE Y,et al. Multitargeted inhibition of hepatic fibrosis in chronic iron-overloaded mice by Salvia miltiorrhiza[J]. J Ethnopharmacol,2013,148(2):671-681.
[8] 刘明家,周志强,祖元刚,等. 湿法消解-火焰原子吸收法测定动物样品中六种金属元素[J]. 光谱学与光谱分析,2012,32(7):1961-1964.
[9] TAN T C,CRAWFORD D H,JASKOWSKI L A,et al. A corn oil-based diet protects against combined ethanol and iron-induced liver injury in a mouse model of hemochromatosis[J]. Alcohol Clin Exp Res,2013,37(10):1619-1631.
[10] 陆美婷,张立威,习文韬,等. 大鼠非酒精性脂肪性肝炎模型的铁状态[J]. 江苏大学学报(医学版),2016,26(4):277-282.
[11] 赵卫华,王燕红,丛敏,等. 铁和铁调素在四氯化碳肝纤维化小鼠模型中的表达[J]. 临床和实验医学杂志,2017,16(4):313-316.
[12] 江远,张玲,何金洋,等. 在二甲基亚硝胺诱导大鼠肝纤维化时铁超载和脂肪堆积的作用[J]. 中西医结合肝病杂志,2009,19(2):100-102.
[13] BRIDLE K R,CRAWFORD D H,FLETCHER L M,et al. Evidence for a sub-morphological inflammatory process in the liver in haemochromatosis[J]. J Hepatol,2003,38(4):426-433.
[14] BOMFIM E A,PEREIRADE DE OLIVEIRA A C,PARAN?魣R, et al. A study on hepatic iron overload in chronic hepatitis C patients[J]. Acta Gastroenterol Latinoam,2013,43(3):212-217.
[15] 李强,陈明,汪莉萍,等. 乙型肝炎肝硬化(下转第41页)(上接第23页)患者血清铁和铁蛋白水平与脂质过氧化损伤的相关性研究[J]. 实用肝脏病杂志,2009,12(1):28-30.
[16] 江宇泳,韩梅丽. 慢性乙型肝炎与乙肝肝硬化血清铁代谢指标的比较[C]//第二十三次全国中西医结合肝病学术会议论文汇编. 贵阳:中国中西医结合学会肝病专业委员会,2014:1.
[17] 罗梅宏. 从“脾主运化”和“运脾生血”理论探讨慢性病贫血的中医病机和治疗[J]. 中医杂志,2013,54(18):1556-1557.
[18] 郑秦,管宇,王志成,等. 异功散对脂多糖介导的小鼠铁代谢紊乱的影响[J]. 中医杂志,2015,56(20):1767-1770.
[19] 葛冰景,肖铁刚,周薏,等. 逍遥散联合恩替卡韦治疗肝郁脾虚型乙肝后肝硬化代偿期30例临床研究[J]. 江苏中医药,2020,52(11):28-31.
[20] 张辉凯,罗宏伟,孟祥林,等. 逍遥散对肝纤维化模型大鼠肝功能、肝纤维化指标的影响[J]. 中医学报,2019,34(2):324-327.
[21] 贾璐,柯旺,李襄,等. 普洱茶醇提物对非酒精性脂肪性肝病小鼠的肝保护作用研究[J]. 云南中医学院学报,2020,43(4):8-13.

备注/Memo

备注/Memo:
收稿日期: 2020 - 12- 06基金项目: 上海市青年科技英才扬帆计划(19YF1445200),上海市卫健委中发办青年基金(2018LQ016),上海中医药大学预算内项目(18LK074);上海虹口区卫健委临床重点扶持专科建设项目(HKZK2020A01)第一作者简介: 周薏(1984-),女,主治医师,硕士,研究方向:消化系统疾病的临床与基础研究。△通信作者: 阙任烨,E-mail: 824492@qq.com
更新日期/Last Update: 1900-01-01