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姜黄素催化生姜提取物的光化学反应及抗菌活性研究(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2021年01期
页码:
24-29
栏目:
实验研究
出版日期:
2021-06-28

文章信息/Info

Title:
Studies on Curcumin-catalyzed Photochemical Reaction of Zingiber officinale Extract and Its Antimicrobial Activity
文章编号:
1000-2723(2021)01-0024-06
作者:
黎晓菊1张晓梅1△庹呈杰1张科涛1秦杰琛2张 冰1黄之镨1赵 庆1△
(1. 云南中医药大学,云南 昆明 650500;2. 昆明医科大学,云南 昆明 650500)
Author(s):
LI Xiaoju1 ZHANG Xiaomei1 TUO Chengjie1 ZHANG Ketao1 QIN Jiechen2ZHANG Bing1 HUANG Zhipu1 ZHAO Qing1
(1. Yunnan University of Medicine, Kunming 650500, China 2. Kunming Medical University, Kunming 650500, China)
关键词:
生姜姜黄素光化学反应抗菌活性
Keywords:
Zingiber officinale curcumin photochemical reaction antimicrobial activity
分类号:
R285
DOI:
10.19288/j.cnki.issn.1000-2723.2021.01.003
文献标识码:
A
摘要:
目的研究生姜提取物在姜黄素催化下的光化学反应,以及化学成分与抗菌活性的变化。方法 采用纸片扩散法,测试光照后的生姜提取液,加入姜黄素、未光照的生姜提取液,加姜黄素并经过光照的生姜提取液,对23种病原菌的抗菌活性。采用薄层色谱法与高效液相色谱法检测加入姜黄素的生姜提取液光照后化学成分的变化。结果 加入姜黄素的生姜提取液经光照处理后,对大多数菌株的抗菌活性明显增强,尤其对耐甲氧西林金黄色葡萄球菌(MRSA)及耻垢分枝杆菌的抗菌活性增强显著。薄层色谱和高效液相色谱显示加入姜黄素的生姜提取液在光照后,化学成分发生了明显变化。结论 在姜黄素的催化下,生姜的化学成分在光照时发生光化学反应,生成了抗菌活性更高的产物。
Abstract:
Objective Photochemical reaction of Zingiber officinale extract catalyzed by curcumin, and antimicrobial activities of the extracts before and after illumination were studied. Methods Disk diffusion test was applied to test the antimicrobial activities of the extract without curcumin after illumination, the extract containing curcumin after illumination, and that containing curcumin without illumination, against 23 kinds of microbial pathogens. TLC and HPLC were applied to detect the changes of the components in the Zingiber officinale extract after illumination, with or without curcumin. Results The antimicrobial activity of the illuminated extract containing curcumin increased significantly, especially the activities against MRSA and Mycobacterium smegmatis increased drastically. TLC and HPLC revealed that the components in Zingiber officinale extract containing curcumin changed significantly after illumination. Conclusion Under the catalysis of curcumin, some compounds in Zingiber officinale underwent photochemical reaction after illumination. As a result, products with much higher antimicrobial activity were generated.

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备注/Memo

备注/Memo:
收稿日期: 2020 - 12- 18基金项目: 国家自然科学基金(81460533,21662048,81060262);云南省科技厅-云南中医学院应用基础研究联合专项基金[2017FF116(-011)]第一作者简介: 黎晓菊(1996-),女,在读硕士研究生,研究方向:天然药物化学。△通信作者: 赵庆,E-mail: qingzhaokm2008@126.com;张晓梅,E-mail:meimeizhang.net@163.com
更新日期/Last Update: 1900-01-01