我们的网站为什么显示成这样?

可能因为您的浏览器不支持样式,您可以更新您的浏览器到最新版本,以获取对此功能的支持,访问下面的网站,获取关于浏览器的信息:

|本期目录/Table of Contents|

75%摄食量对尿酸酶缺失大鼠的降尿酸作用*(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2021年05期
页码:
7-12
栏目:
实验研究
出版日期:
2022-09-20

文章信息/Info

Title:
Serum Uric Acid Lowered by 75% Dietary Intake in Uricase-deficient Rats
文章编号:
1000 - 2723(2021)05- 0007 - 06
作者:
綦雅琳1秦婉1杲银芳2范楠2万旭莲1云宇2段为钢1
(1. 云南中医药大学 基础医学院,云南 昆明 650500;2. 昆明医科大学 基础医学院药理学系,云南 昆明 650500)
Author(s):
QI Yalin1 QIN Wan1 GAO Yinfang2 FAN Nan2 WAN Xulian1 YUN Yu2 DUAN Weigang1
(1. School of Basic Medicine, Yunnan University of Chinese Medicine, Kunming 650500, China; 2. The Faculty of Pharmacology, School of Basic Medicine, Kunming Medical University, Kunming 650500, China)
关键词:
尿酸酶缺失昆明DY 大鼠高尿酸血症摄食量
Keywords:
uricase deficiency Kunming-DY rat hyperuricemia food intake
分类号:
R589.7
DOI:
10.19288/j.cnki.issn.1000-2723.2021.05.002
文献标识码:
A
摘要:
为观察不同摄食量对尿酸酶缺失大鼠(昆明DY大鼠)血尿酸影响,将45 d日龄雄性昆明DY大鼠随机分成禁食组(禁食2d)或节食组(即给予正常摄食量的90%、80%、75%、70%、60%、50%、25%,持续14 d);实验前、中、后断尾取血制备血清,记录体质量、24 h摄食和饮水量,收集大鼠24 h尿液和粪便,并使用磷钨酸法检测样品尿酸含量。结果表明,禁食2 d会使大鼠血尿酸会显著升高,每日给予大鼠正常摄食量的75%,其血尿酸、肌酐、总胆固醇和甘油三酯14 d后呈显著下降趋势,且体质量维持稳定;其余不同摄食量的组分对大鼠血尿酸有不同程度的增加倾向或无明显影响。综上,摄食量控制在75%,对尿酸酶缺失大鼠具有降尿酸的作用,同时对肾功和血脂也有一定的改善作用。
Abstract:
In order to observe the effect of food intake on serum uric acid(SUA), male uricase-deficient rats (Kunming-DY rats) aging 45 days were randomly divided into fasting group (fasting for 2 days) or dieting groups (90%, 80%, 75%, 70%, 60%, 50% and 25% of normal food intake for 14 days). Before, during and after the experiment, blood samples were drawn from the tail to prepare serum. In the same time, body weight, 24-hour food intake and water consumption were recorded, and 24-hour urine and feces of rats were collected. The uric acid in samples was detected by uric acid assay kits of phosphotungstic acid method. The results showed that SUA in fasting group significantly increase. SUA in the rats that received 75% of the normal food supply was lowered. Besides, creatinine, total cholesterol and triglyceride in their serum decreased significantly after 2-weeks' treatment, with their stable body weight. Other food intakes had almost no significant effect on, or increase SUA in Kunming-DY rats. In conclusion, 75% food intake lower SUA in uricase-deficient rats with their improvement of renal function and blood lipid.

参考文献/References

[1] ICHIDA K,MATSUO H,TAKADA T,et al. Decreased extra-renal urate excretion is a common cause of hyperuricemia[J]. Nat Commun,2012(3):764.
[2] 张宏,王旭昀,吕翠岩,等. 血尿酸的来源去路及痛风关节炎的中医辨治[J]. 医学综述,2018,24(8):1617-1620.
[3] MAIUOLO J,OPPEDISANO F,GRATTERI S,et al. Regulation of uric acid metabolism and excretion[J]. Int J Cardiol,2016(213):8-14.
[4] ZENG J,ZHANG J G,LI Z Y,et al. Prediction model of artificial neural network for the risk of hyperuricemia incorporating dietary risk factors in a Chinese adult study[J]. Food Nutr Res,2020(20):64.
[5] GAO Y,CUI L F,SUN Y Y,et al. Adherence to the Dietary Approaches to Stop Hypertension Diet and Hyperuricemia:A Cross-Sectional Study[J]. Arthritis Care Res,2021,73(4):603-611.
[6] 岳俊林,张娇华. 痛风和高尿酸血症患者饮食控制知性行调查研究[J]. 人人健康,2020(6):51.
[7] WANG Y F,ZHAO L,GAO L W,et al. Health policy and public health implications of obesity in China[J]. Lancet Diabetes Endocrinol,2021,9(7):446-461.
[8] DEHLIN M,JACOBSSON L,RODDY E. Global epidemiology of gout: prevalence, incidence, treatment patterns and risk factors[J]. Nat Rev Rheumatol,2020,16(7):380-390.
[9] ZHOU M M,YANG N,XING X,et al. Obesity interacts with hyperuricemia on the severity of non-alcoholic fatty liver disease[J]. BMC Gastroenterol,2021,21(1):43.
[10] 张伯礼,吴勉华. 中医内科学[M]. 4版. 北京:中国中医药出版社,2017:363-368.
[11] 杨保林,丁岗. 高尿酸血症中医论治已见[J]. 中国中医药现代远程教育,2010,8(9):20-21.
[12] 钱玉中,苏于纳,李娜,等. 论高尿酸血症的中医治疗思路[J]. 湖南中医药大学学报,2012,32(12):21-22.
[13] 张秋平,庞新颖,陈丽辉,等. 从中医阴火角度论治高尿酸血症[J]. 河南中医,2018,38(7):1047-1049.
[14] 张阳,郭宏敏. 络病理论在高尿酸血症中的应用[J]. 陕西中医,2011,32(12):1638-1639.
[15] 肖瑶,赵进喜. 从肝论治高尿酸血症的探讨[J]. 环球中医药,2018,11(1):104-106.
[16] 刘美茜,刘长平,金香淑. 中医药治疗高尿酸血症的进展[J]. 中国中医药现代远程教育,2019,17(18):147-149.
[17] 张楠,胡欣瑜,董鲜祥,等. 高尿酸血症动物模型的研究进展[J]. 昆明医科大学学报,2019,40(6):129-134.
[18] LU J,HOU X,YUAN X,et al. Knockout of the urate oxidase gene provides a stable mouse model of hyperuricemia associated with metabolic disorders[J]. Kidney Int,2018,93(1):69-80.
[19] YU Y,ZHANG N,DONG X X,et al. Uricase-deficient rat is generated with CRISPR/Cas9 technique[J]. PeerJ,2020,8:e8971.
[20] 张楠,懂鲜祥,云宇,等. 尿酸酶缺失 Kunming-DY 大鼠的研制[J]. 中国药理学与毒理学杂志,2019,33(10):931.
[21] TINSLEY G M,LA BOUNTY P M. Effects of intermittent fasting on body composition and clinical health markers in humans[J]. Nutr Rev,2015,73(10):661-674.
[22] MOJTO V,GVOZDJAKOVA A,KUCHARSKA J,et al. Effects of complete water fasting and regeneration diet on kidney function,oxidative stress and antioxidants[J]. Bratisl Lek Listy,2018,119(2):107-111.
[23] Z?譈RCHER H U,MEIER H R,HUBER M,et al. Acute kidney failure as a complication of fasting therapy[J]. Schweiz Med Wochenschr,1977,107(29):1025-1028.

备注/Memo

备注/Memo:
收稿日期: 2021 - 09- 23
* 基金项目: 云南省中医药联合专项重点项目(202101AZ070001-010)
第一作者简介: 綦雅琳(1995-),女,在读硕士研究生,研究方向:中医药学研究新技术与新方法。
△通信作者: 段为钢,E-mail:deardwg@126.com
更新日期/Last Update: 1900-01-01