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高脂饮食致非酒精性脂肪性肝病大鼠小肠、肝脏中NOD样受体蛋白3及相关炎症因子的表达*(PDF)

《云南中医学院学报》[ISSN:1000-2723/CN:53-1048/R]

期数:
2021年05期
页码:
13-18
栏目:
实验研究
出版日期:
2022-09-20

文章信息/Info

Title:
Expression of Nod-like Receptor Protein 3 in Intestine and Liver of Rats with Nonalcoholic Fatty Liver Disease Induced by High-fat Diet
文章编号:
1000 - 2723(2021)05- 0013 - 06
作者:
夏恩蕊张素妍田格格李浩杜玉锐张顺贞△
(云南中医药大学中药学院,云南 昆明 650500)
Author(s):
XIA Enrui ZHANG Suyan TIAN Gege ZHAGN Shunzhen
(School of Traditional Chinese Medicine, Yunnan University of Chinese Medicine, Kunming 650500, China)
关键词:
非酒精性脂肪性肝病NOD样受体蛋白3 炎症小体炎症因子
Keywords:
nonalcoholic fatty liver disease NOD-like receptor protein 3 inflammasomes inflammatory cytokines
分类号:
R575.5
DOI:
10.19288/j.cnki.issn.1000-2723.2021.05.003
文献标识码:
A
摘要:
目的 观察高脂饮食致非酒精性脂肪性肝病(NAFLD)大鼠小肠、肝脏中NOD样受体蛋白3(NLRP3)及相关炎症因子表达情况。方法 SD雄性大鼠20只适应性喂养1周后,随机分为正常组、模型组,每组10只,正常组普通饲料喂养,模型组高脂饲料喂养。8周末取材,观察肝HE及油红O染色病理切片,检测各组血清和肝脏中谷丙转氨酶(ALT)、谷草转氨酶(AST)、胆固醇(TC)、甘油三酯(TG)、白介素-18(IL-18)、白介素-1β(IL-1β)。RT-PCR、免疫组化检测肝、肠NLRP3表达。结果 与正常组相比,模型组大鼠肝脏表现出明显的脂肪变性和炎性病变,肝脏、血清中ALT、AST、TG显著升高(P<0.05),TC升高,差异不明显,IL-18、IL-1β显著升高(P<0.05);肝脏、小肠NLRP3表达量亦显著升高(P<0.05)。结论 NAFLD可引起大鼠小肠、肝脏中NLRP3及相关炎症因子上调。
Abstract:
Objective To observe the expression of NOD-like receptor protein 3(NLRP3) and related inflammatory factors in the small intestine and liver of non-alcoholic fatty liver disease (NAFLD) rats caused by high-fat diet. Methods After one week of adaptive feeding, 20 SD male rats were randomly divided into a normal group and a model group with 10 rats in each group. The normal group was fed with ordinary diet and the model group was fed with high-fat diet. Samples were collected at the end of the 8th week to observe liver HE and Oil Red O stained pathological sections, and detect alanine aminotransferase (ALT), aspartate aminotransferase (AST), cholesterol (TC), triglycerides (TG), and interleukin 18 (IL) in serum and liver -18), interleukin 1β (IL-1β). Detect the expression of NLRP3 in liver and intestine. Results Compared with the normal group, the liver of the model group showed obvious steatosis and inflammatory lesions. The ALT, AST and TG in the liver and serum were significantly increased(P<0.05), and the TC was increased, but the difference was not significant. IL-18 and IL-1β were significantly increased(P<0.05); the expression of NLRP3 in the liver and small intestine was also significantly increased(P<0.05). Conclusion NAFLD can cause up-regulation of NLRP3 and related inflammatory factors in the small intestine and liver of rats.

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备注/Memo

备注/Memo:
收稿日期: 2021 - 06- 18
* 基金项目: 国家自然科学基金(81760818);云南省高校中医证候微观辨证重点实验室(20190720);云南省科技厅中医联合专项[2018FF001(-042)];云南省科技厅中医联合专项-重点项目(202101AZ070001-008)
第一作者简介: 夏恩蕊(1997-),女,在读硕士研究生,研究方向:中医药防治肝胆疾病。
△通信作者: 张顺贞,E-mail:694415176@qq.com
更新日期/Last Update: 1900-01-01