Objective To explore the effect of "Dredging hub, divergenting serum and refinding turbid" method, namely the application of Sansheng Tiaozhishu to improve the blood glucose fluctuation and intestinal glucagon-like peptide-1 (GLP-1) secretion level in diabetic GK rats. Methods A rat model of diabetic macroangiopathy was established by high-fat diets. The rats were divided into 4 groups: blank control group, model group, Sansheng Tiaozhishu group and positive control group. After intragastric administration, their blood glucose levels were measured once a week at 8:00, 10:00, 14:00, 16:00 and 18:00, and their blood glucose stability indexes (MBG, SDBG and LAGE) were used to quantify the blood glucose stability of each group. The levels of serum GLP-1, serum insulin and glucagon were measured. Results The condition of Sansheng Tiaozhishu group was better than that of the model group, which could reduce the fluctuation of blood glucose and increase the level of serum GLP-1. Compared with the model group, insulin (INS) had no statistical significance but GLP-1 was significantly higher than that of the model group (P<0.01). Conclusion The application of the method of "Dredging hub, divergenting serum and refinding turbid" in the treatment of diabetic GK rats with the Sansheng Tiaozhishu in our hospital can promote the secretion of GLP-1, reduce blood sugar and the fluctuation of blood sugar, so as to maintain the homeostasis of blood glucose, alleviate the injury of vascular endothelium in model GK rats, and improve the pathological changes of the large blood vessels of the disease, thereby slowing the progression of diabetes.